REPARATIVE PROCESSES PROMOTION IN SPORT INJURIES WlTH BONE LESIONS

INTRODUCTION- The injuries of bones in maxillofacial region as a result of traumas in sport are not uncommon. Implantation of apatites containing glasscristalmaterials (AGM) in granules has been suggested for promotion of reparative processes and rehabilitation of athletes with bone defects. The aim of the work is comparative study of osteoinductive characteristics of AGM and composite material based on collagen sponge with 80% AGM (CSAGM)."Biositallw (Russia) has been used as AGM, and "Biositacol" (Russia) - as a CSAGM. The experiment was made on 18 rabbits (3 groups). In 1' and 2nd groups the material have been implanted into perforated holes with diameter 2 mni and 5 mm in depth, located in mandible along the alveolar ridge between incisors and posterior teeth. One group was used as a control one (perforated hole has been filled with blood clots).The samples of implanted materials were taken in 3,7 day, 2,4,6,8 weeks after implantation. Osteoinductive characteristics have been investigated by histomorphologic technique according to osteoblast formation rate and site, their maturation into osteocytes which further formed bone trabeculas with bone plates construction. RESULTS-In the control group of animals whose mandible perforations had been allowed to fill with blood clots, histologic examinations showed acute inflammation reactions in the periphery of the bone defects after seven days. After 2-4 weeks the reproduction of fibroblasts and formation of conjuctive tissue in the bone defect were observed. By the sixth week, the jaw bone defect was filled in with well formed mature conjunctive tissue, and by the eighth week isolated bone tissue growths were observed. In the early stage of the experiment, 3-7 days after implantation, acute inflammation with lymphohisteocytic reaction around the AGM granules was recorded. At the same time, by the second week muttinucleated cells of foreign bodies appeared in all test groups. By the forth week, tymphohisteocytic infiltrates disappeared, and the multinucleated cells of foreign bodies had appeared to resolve the AGM granules. Surrounding the AGM granules, a great number of osteocytes had formed young conjunctive tissue which incapsuled almost every granule. By the sixth week, the histological preparations showed the formation of bone tissue in the periphery of the defect. Regenerated bone spicules in the process of mending developed the spongy structure of bone tissue. By the eighth week, the process of osteogenesis around the AGM granules was far advanced everywhere. During the implantation of CSAGM the extensive osteoclastic response at the defect borders was defined by the 3d day. From the 7Ih day to two weeks single osteoblasts appeared and proliferation of immature cells of collagen tissue was noted. By the second week fibroblast net between AGM granules as well as single new-formed collagen fibers and single bone trabeculas at the defect borders appear. During the 4'h week the defect periphery was filled with bone trabeculas with their partial growth towards the centre. By the 6Ih week osteogenesis was marked all over the defect. CONCLUSION-Both AGM and CSAGM implantation promotes reparative processes in bone injuries comparing with the control group. In CSAGM implantation histomorphological pattern of bone tissue elements formation appears in two weeks earlier than in AGM implantation. The difference in direction of reparative processes in bone has been noted: all over the defect in the zone of AGM implantation and at the defect borders in CSAGM implantation

Academic stress and its effect on medical students’ mental health status

This review aimed to analyze the data on features of a medical students’ mental status and its dynamics in the course of study. The analysis was applied to the scientific publications (mainly over the past 5 years) on the issue of academic stress and medical students’ mental status in different countries from computer databases: PubMed, Medical-Science, eLibrary, Web of Science, and Scopu

Development and implementation of special seminar “Innovative pedagogical technologies”

Taking into account modern requirements to teacher’s personality in primary education, his knowledge of innovative pedagogical technologies’ essence as well as knowledge of methodology,  relevance of the search for ways to improve the content and forms of future primary school teachers’ training has been revealed. One of the solutions to this problem can be introduction of a special seminar aimed at improving the level of innovative and technological competence of future teachers in primary education into the system of vocational training. The purpose of this article is to substantiate the conceptual and methodological significance of a special seminar "Innovative pedagogical technologies" as well as to describe process of diagnosing effectiveness of the developed special seminar. The aim of the research is achieved through the application of such theoretical and empirical methods of scientific knowledge as method of pedagogical diagnostics, methodical system modeling, method of pedagogical forecasting, analysis of results, synthesis of the results of experimental research. Based on the use of these methods, the main theoretical aspects of a special seminar development are presented, the components of its content and structure are reflected and the stages of testing effectiveness of the developed special seminar are described. The scientific novelty of the research is justified by the development of the conceptual foundations and content of the special seminar "Innovative pedagogical technologies", intended for use in the training of future primary school teachers, indicating methodological aspects of its implementation, as well as the development of a diagnostic system to test its effectiveness in the educational process of the University

Photometric determination of thiourea with the use of sodium nitroprusside

Conditions for the formation of the colored compound in the reaction of thiourea with sodium nitroprusside and the products of its transformations were studied. A procedure for the photometric determination of thiourea was developed. The procedure is based on the complexation of thiourea with pentacyanoferrate(II) ion, which can be obtained by the basic hydrolysis of sodium nitroprusside at pH ≥ 12, followed by acidification of the solution to pH 6. When the reagent is present in excess, the absorbance of the solution of the complex at 590 nm is linearly proportional to the thiourea concentration in the solution within the range 4 × 10-6-1 × 10-4 M. © 1997 MAEe Cyrillic signK Hayκa/Interperiodica Publishing

Chronic cholecystitis at patients with the various trophological status: mechanisms and feature of clinical course

Aim of investigation. To analyze clinical, biochemical features of various forms of pathology of a gallbladder in view of features of food behavior and factors of its regulation at patients with the various trophological status.Material and methods. Overall 167 patients with gallbladder diseases and various trophological status were included in original study. Food behavior analysis was carried out, clinical features, anthopometry were determined, bile lipid spectrum, serum content of leptin and insulin were studied by enzyme-linked immunoassay.Results. Investigation of gallbladder diseases has objectively demonstrated, that trophological status affects development and course of chronic cholecystitis, but is not the only determining factor.Conclusions. Development and course of gallbladder diseases are defined by trophological status based on disorders of food behavior, change of production of leptin, insulin

Optimization of Nanostructuring Burnishing Technological Parameters by Taguchi Method

On the basis of application of Taguchi optimization method, an approach for researching influence of nanostructuring burnishing technological parameters, considering the surface layer microhardness criterion, is developed. Optimal values of burnishing force, feed and number of tool passes for hardened steel AISI 420 hardening treatment are defined

Identification of duplicate accessions in the pea (<i>Pisum sativum</i> L.) collection at VIR

Background. Identification of duplicates in the collections of genetic resources is the most important problem of seed gene bank management. Duplicate accessions expand the collection size, thus raising the costs of germplasm maintenance without broadening the genetic diversity.Materials and methods. The studied material included 17 pairs of Pisum sativum L. accessions from the VIR collection which presumably had been erroneously registered twice in the VIR catalogue; however, they had identical introductory numbers. The accessions entered the collection in 1922–1996 and to date they have been reproduced 2 to 16 times. After a field assessment, 15 pairs of putative duplicate accessions of various uses were selected for molecular analysis. A RAPD analysis was performed using five primers from the Operon Series. Total seed proteins were analyzed by SDS-polyacrylamide gel electrophoresis.Results. The following criteria were used to ascertain identity of the accessions or their difference: 1) similarity of morphological characters (habitus, and anthocyanin pigmentation of flowers and vegetative organs) and flowering dates; 2) identity or polymorphism of RAPD profiles; and 3) identity or difference in electrophoretic banding patterns of seed storage proteins. Seven pairs of duplicates were identified according to the results of a comparative analysis. Among them, the accessions in the pairs k-81/k-1199, k-8331/k-8645, k-8719/k-8760, and k-8757/k-8825 turned out to be completely identical, while k8464/k-8472, k-8740/k-8873, and k-8689/k-8723 were heterogenic, but had similar RAPD profiles and seed proteins patterns.Conclusions. An integrated assessment involving in-field plant phenotyping and analyzing polymorphism of amplified DNA fragments and components in electrophoretic banding patterns of seed proteins is promising for detecting identical or heterogenic accessions in genebank collections