22 research outputs found
Dynamics of Air-Fluidized Granular System Measured by the Modulated Gradient Spin-echo
The power spectrum of displacement fluctuation of beads in the air-fluidized
granular system is measured by a novel NMR technique of modulated gradient
spin-echo. The results of measurement together with the related spectrum of the
velocity fluctuation autocorrelation function fit well to an empiric formula
based on to the model of bead caging between nearest neighbours; the cage
breaks up after a few collisions \cite{Menon1}. The fit yields the
characteristic collision time, the size of bead caging and the diffusion-like
constant for different degrees of system fluidization. The resulting mean
squared displacement increases proportionally to the second power of time in
the short-time ballistic regime and increases linearly with time in the
long-time diffusion regime as already confirmed by other experiments and
simulations.Comment: 4 figures. Submited to Physical Review Letters, April 200
Molecular velocity auto-correlation of simple liquids observed by NMR MGSE method
The velocity auto-correlation spectra of simple liquids obtained by the NMR
method of modulated gradient spin echo show features in the low frequency range
up to a few kHz, which can be explained reasonably well by a long
time tail decay only for non-polar liquid toluene, while the spectra of polar
liquids, such as ethanol, water and glycerol, are more congruent with the model
of diffusion of particles temporarily trapped in potential wells created by
their neighbors. As the method provides the spectrum averaged over ensemble of
particle trajectories, the initial non-exponential decay of spin echoes is
attributed to a spatial heterogeneity of molecular motion in a bulk of liquid,
reflected in distribution of the echo decays for short trajectories. While at
longer time intervals, and thus with longer trajectories, heterogeneity is
averaged out, giving rise to a spectrum which is explained as a combination of
molecular self-diffusion and eddy diffusion within the vortexes of hydrodynamic
fluctuations.Comment: 8 pages, 6 figur
Utilização de eletroquimioterapia para carcinoma de células escamosas tegumentar em felino
Introduction of a new model for time-continuous and non-contact investigations of in-vitro thrombolysis under physiological flow conditions
<p>Abstract</p> <p>Background</p> <p>Thrombolysis is a dynamic and time-dependent process influenced by the haemodynamic conditions. Currently there is no model that allows for time-continuous, non-contact measurements under physiological flow conditions. The aim of this work was to introduce such a model.</p> <p>Methods</p> <p>The model is based on a computer-controlled pump providing variable constant or pulsatile flows in a tube system filled with blood substitute. Clots can be fixed in a custom-built clot carrier within the tube system. The pressure decline at the clot carrier is measured as a novel way to measure lysis of the clot. With different experiments the hydrodynamic properties and reliability of the model were analyzed. Finally, the lysis rate of clots generated from human platelet rich plasma (PRP) was measured during a one hour combined application of diagnostic ultrasound (2 MHz, 0.179 W/cm<sup>2</sup>) and a thrombolytic agent (rt-PA) as it is commonly used for clinical sonothrombolysis treatments.</p> <p>Results</p> <p>All hydrodynamic parameters can be adjusted and measured with high accuracy. First experiments with sonothrombolysis demonstrated the feasibility of the model despite low lysis rates.</p> <p>Conclusions</p> <p>The model allows to adjust accurately all hydrodynamic parameters affecting thrombolysis under physiological flow conditions and for non-contact, time-continuous measurements. Low lysis rates of first sonothrombolysis experiments are primarily attributable to the high stability of the used PRP-clots.</p
Electrochemotherapy: technological advancements for efficient electroporation-based treatment of internal tumors
A mathematical model for the dissolution of non-occlusive blood clots in fast tangential blood flow
Abstract. Our aim was to study the effect of an axially directed blood plasma flow on the dissolution rate of cylindrical nonocclusive blood clots in an in vitro flow system and to derive a mathematical model for the process. The model was based on the hypothesis that clot dissolution dynamics is proportional not only to the biochemical proteolysis of fibrin but also to the power of the flowing blood plasma dissipated along the clot. The predicted rate of thrombolysis is then proportional to the square of the average blood plasma velocity for laminar flow and to the third power of the average velocity for turbulent flow. To verify the model, the time dependence of the clot cross-sectional area was measured by dynamic magnetic resonance microscopy during fast (turbulent) and slow (laminar) flow of plasma through an axially directed channel along the clot. The flowing plasma contained a magnetic resonance imaging contrast agent (Gd-DTPA) and a thrombolytic agent (recombinant tissue-type plasminogen activator). The experimental data fitted well to the model, and confirmed the predicted increase in the dissolution rate when blood flow changed from a laminar to a turbulent flow regime
Comparison of single-shot rapid acquisition with relaxation enhancement and echo planar current density MRI sequences for monitoring of electric pulse delivery in irreversible electroporation
Success of electroporation treatment critically depends on coverage of the target tissue with electric field. The electric field during delivery of the electroporation pulses can be reconstructed by the magnetic resonance electric impedance tomography, a method that uses current density distribution data and electric potentials at the electrodes for reconstruction of electric field in the sample. In this study, two complementary MRI methods for current density imaging during delivery of irreversible electroporation pulses are presented. One of the methods is based on the single-shot rapid acquisition with relaxation enhancement while the other is based on the echo planar imaging MRI method. The methods were compared in terms of their sensitivity and susceptibility to image artifacts by experiments on a liver test sample that were performed on a 2.35 T small bore MRI scanner. In the experiments, a standard protocol for irreversible electroporation where 90 electric pulses of 100 μs, 3000 V are delivered at 1 Hz was performed. Results of the study confirmed that both methods have comparable sensitivity. The RARE-based method was found less susceptible to artifacts while the EPI-based method has lower SAR value and may therefore be a better candidate for use in clinics