Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection

At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two modifications versus the mechanism of the results registration (FRT - real time and FEP - end-point). High diagnostic value of the reagent set is determined in medical trials at L.A. Tarasevich Institute. The set is registered as medical device

Neuroendocrinal Aspects of Analysis of Biomodel Lymphoid Organs Response to Administration of Live Tularemia Vaccine

Evaluated were alterations in thymus, regional and remote lymphatic glands, and spleen of biomodels as well as neuroendocrinal cells reaction in these organs in simulating anti-tularemia vaccinal process. Received are quantitative characteristics of neuroendocrinal environment state of biomodels' lymphoid organs on the 5 day after administration of anti-tularemia vaccine in different doses. Elucidated were certain principles in cells' reaction of APUD-system of biomodel's lymphoid organs and lungs, associated with nature of their alterations. Identified were the prospects of application of the APUD-system cells quantitative characteristics in view of improving and optimizing the evaluation methods as regards safety and efficiency of live vaccines

Assessment of “Specific Safety” Index of the Live Tularemia Vaccine

Analyzed were the results of specific safety control of the live tularemia dry vaccine used for tularemia prophylaxis. During the period of 1997-2006 this index was shown to be unstable. Specific safety of the vaccine is one of the main parameters of its quality, and in cases it does not meet the requirements of the normative documentation (ND) the vaccine is rejected during the control. It was proposed to introduce amendments in ND and to use for vaccine control standard animals, the conditions of their keeping being strictly observed