9 research outputs found
Immunological factor development of external genital endometriosis
External genital endometriosis (EGE) is one of the common gynecological diseases of women of reproductive age with a relapsing, progressive course that worsens the quality of life of patients due to pain, emotional imbalance, fear of relapse and possible surgical intervention. Currently, endometriosis is recognized as one of the most common diseases associated with infertility. Thus, among fertile women with preserved childbearing function, the disease is generally diagnosed in approximately 6-7%, while among patients suffering from infertility, its frequency can reach 20-48%.However, the causes that determine reproductive dysfunction in patients with EGE are not well understood. Much attention is currently paid to the role of immunity in the formation of endometriosis. Patients with EGE show changes in both local immunity factors and immunological components of circulating blood.Purpose of the study: the study of factors of innate and adaptive immunity in patients of reproductive age with external genital endometriosis (EGE).The study included 71 patients with various stages of external genital endometriosis, the control group included 24 patients without endometriosis. Determination of the population composition of peripheral blood lymphocytes, the level of monocytes expressing TLR, activation markers, was carried out by laser flow cytometry β Immunotex (France), Caltag (USA), FITC (fluorescein isothiocynate) β labeled CD3, CD4, CD8, CD16, CD19, HLA-DR, CD282, CD284 and PE (phycoerythrin) - labeled with CD25, CD69, CD95, CD107a, CD14.External genital endometriosis is characterized by: at stages I-II of the disease - a violation of the early stages of the innate immune response (an increase in the number of monocytes expressing TLR-4, a violation of the activation and differentiation processes of immunocompetent cells, which is reflected in a decrease in the expression of CD16, CD8, CD16+HLA-DR+, CD16+CD107a+, CD8+CD107a+, at III-IV stages of the disease, there is a decrease in the level of CD16 and activation markers CD69, HLA-DR, CD107a on their surface, which is combined with a decrease in the expression of CD8, CD16, HLADR and CD107a on their surface. CD95+ and CD8+CD95+ were found at various stages of EGE.The results obtained allow us to understand the features of the functioning of innate and adaptive immunity at various stages of external genital endometriosis, and the studied immunological parameters can be used as diagnostic criteria for the formation of various stages of EGE. These data can serve as a theoretical basis for further identification of markers of EGE progression, as well as the mechanisms underlying immune inflammation
FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) β newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNΞ³, IFNΞ±, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNΞ±, IFNΞ³, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNΞ±, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns
Features of the cytokine profile in adolescents with microvascular complications of type 1 diabetes mellitus
Despite advances of modern medical science, the consequences associated with management of complications in type 1 diabetes mellitus (DM1) in children and adolescents represent a serious problem. Common development of microvascular diabetic complications (retinopathy, neuropathy, kidney damage) still remains a sufficient obstacle for achieving high quality of life and social adaptation in the young patients, thus promoting studies of immune mechanisms involved in genesis of microvasculature damage under the conditions of dysmetabolic abnormalities associated with DM1. Our goal was to assess the role of altered cytokine balance in blood serum in development of microangiopathies in adolescents with DM1.140 adolescent patients with type 1 diabetes aged 14-18 years were examined being divided in 2 groups: group I included the patients with glycated hemoglobin (HbA1c) level of > 9.0% (n = 65), and group II which included adolescents with HbA1C level of β€ 9.0% (n = 75). Each group was divided into subgroups: Ia (n = 50) and IIa (n = 38) included adolescents with diabetic retinopathy, nephropathy or neuropathy, whereas groups Ib (n = 15) and IIb (n = 37) were without microvascular complications. The control group consisted of 36 adolescents with normal body weight, without carbohydrate metabolic disorders, and family history of diabetes mellitus. Determination of TNFΞ±, IL-1Ξ², VCAM-1, fractalkine levels in blood serum was performed by enzyme immunoassay using test systems βRayBiotechβ (USA), βBIOSCIENCEβ (USA).Development of microangiopathies in adolescents with different glycemic control is associated with increased serum concentration of the factors involved in neoangiogenesis and vascular wall remodeling, i.e., TNFΞ±, IL-1Ξ², VCAM-1, compared with control group (p < 0.05), and a statistically significant decrease in fractalkine level in adolescent patients with either complicated, or uncomplicated DM1. The study allowed us to suggest that occurrence of microvascular complications in adolescents with DM1 is associated with impaired immune response tending for altered cytokine balance towards Th1 type, enhanced intercellular interactions, imbalance of bioregulatory molecules, contributing to development of inflammatory immunoregulatory state. The revealed patterns of laboratory markers, along with assessment of metabolic indices, will enable personalized approaches to early diagnostics of microvascular complications in adolescents with DM1 and prevent their further progression
ΠΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° Ρ Π΄Π΅ΡΠ΅ΠΉ Π³ΡΡΠ΄Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° Ρ Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈΠΈ Π½Π° ΡΠΎΠ½Π΅ Π³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π¦ΠΠ‘
Objective: based on the production of cytokines, to identify the immunological features of the chronic course of cytomegalovirus infection in children of the first year of life against the background of hypoxic-ischemic CNS damage.Research methods:108 newborns with cytomegalovirus infection occurring against the background of perinatal hypoxic-ischemic lesions of the central unequal system were examined. All observed patients immediately after the diagnosis of cytomegalovirus infection underwent an immunological examination, including the determination of the levels of interferon-alpha (IFN-Ξ±) and interferon-gamma (IFN-Ξ³), the level of interleukins β 2 and 4 (IL -2 and IL-4) necrosis factor human alpha tumors (TNF-Ξ± in blood serum was determined by enzyme immunoassay using a set of reagents ProCon IF2 plus, ProCon Ifgamma, ProCon TNFΞ± (Protein contour LLC, Russia, St. Petersburg). At 1 and 6 months of life .The observation groups consisted of 78 children (72.2%) with an acute course of the disease (Group 1) and 30 children (27.3%) with a chronic course (Group 2). The control group consisted of 15 newborns without herpes virus infection.Results. Of the totality of the studied cytokines, statistically significant for the chronic course of cytomegalovirus infection in children of the first year of life against the background of hypoxic-ischemic CNS damage were found: IL-2, IFN-Ξ³. It was found that in children with a persistent low level of IFN-Ξ³ and an increased level of IL-4 in the blood serum at the age of 6 months, there was a chronic course of cytomegalovirus infection against the background of perinatal hypoxic-ischemic CNS damage.A decrease in IFN-Ξ³ production indicates a congenital or acquired deficiency of the interferon system and can be considered as an indication for long-term interferon replacement therapy.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΈΠ·ΡΡΠΈΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° ΠΏΡΠΈ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠΈ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ, Ρ Π΄Π΅ΡΠ΅ΠΉ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π° ΠΆΠΈΠ·Π½ΠΈ Π½Π° ΡΠΎΠ½Π΅ Π³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π¦ΠΠ‘.ΠΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 108 Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
Ρ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ, ΠΏΡΠΎΡΠ΅ΠΊΠ°Π²ΡΠ΅ΠΉ Π½Π° ΡΠΎΠ½Π΅ ΠΏΠ΅ΡΠΈΠ½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π½Π΅ΡΠ°Π²Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. ΠΡΠ΅ΠΌ Π½Π°Π±Π»ΡΠ΄Π°Π²ΡΠΈΠΌΡΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ ΡΡΠ°Π·Ρ ΠΏΠΎΡΠ»Π΅ ΠΏΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΠ·Π° ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½Π°Ρ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ΅Π΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π°-Π°Π»ΡΡΠ° (ΠΠ€Π-Ξ±) ΠΈ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π°-Π³Π°ΠΌΠΌΠ° (ΠΠ€Π-Ξ³) ΡΡΠΎΠ²Π΅Π½Ρ ΠΈΠ½ΡΠ΅ΡΠ»Π΅ΠΉΠΊΠΈΠ½ΠΎΠ² β 2 ΠΈ 4 (ΠΠ-2 ΠΈ ΠΠ-4), ΡΠ°ΠΊΡΠΎΡΠ° Π½Π΅ΠΊΡΠΎΠ·Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ Π°Π»ΡΡΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° (Π€ΠΠ-Ξ±) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π½Π°Π±ΠΎΡΠ° ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ProConIF2 plus, ProConIfgamma, ProConTNFΞ± (ΠΠΠ Β«ΠΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²ΡΠΉ ΠΊΠΎΠ½ΡΡΡΒ», Π ΠΎΡΡΠΈΡ, Π³. Π‘Π°Π½ΠΊΡ-ΠΠ΅ΡΠ΅ΡΠ±ΡΡΠ³) Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ 1 ΠΈ 6 ΠΌΠ΅ΡΡΡΠ΅Π² ΠΆΠΈΠ·Π½ΠΈ. ΠΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 78 Π΄Π΅ΡΠ΅ΠΉ (72,2%) Ρ ΠΎΡΡΡΡΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ (1 Π³ΡΡΠΏΠΏΠ°) ΠΈ 30 Π΄Π΅ΡΠ΅ΠΉ (27,3%) Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ (2 Π³ΡΡΠΏΠΏΠ°). ΠΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ Π³ΡΡΠΏΠΏΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 15 Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΡΡ
Π±Π΅Π· Π³Π΅ΡΠΏΠ΅ΡΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ· Π²ΡΠ΅ΠΉ ΡΠΎΠ²ΠΎΠΊΡΠΏΠ½ΠΎΡΡΠΈ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π±ΡΠ»ΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΠ΅ Π΄Π»Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ (Π¦ΠΠΠ) Ρ Π΄Π΅ΡΠ΅ΠΉ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π° ΠΆΠΈΠ·Π½ΠΈ Π½Π° ΡΠΎΠ½Π΅ Π³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π¦ΠΠ‘: ΠΠ- 2 ΠΈ ΠΠ€Π-Ξ³. ΠΡΡΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠΎΡ
ΡΠ°Π½ΡΡΡΠΈΠΌΡΡ ΠΏΠΎΠ½ΠΈΠΆΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΠΠ€Π-Ξ³ ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΠΠ-4 Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ 6 ΠΌΠ΅ΡΡΡΠ΅Π² ΠΈΠΌΠ΅Π»ΠΎ ΠΌΠ΅ΡΡΠΎ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Π½Π° ΡΠΎΠ½Π΅ ΠΏΠ΅ΡΠΈΠ½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π¦ΠΠ‘.Π‘Π½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΠ€Π-Ξ³, ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎ Π²ΡΠΎΠΆΠ΄ΡΠ½Π½ΠΎΠΌ ΠΈΠ»ΠΈ ΠΏΡΠΈΠΎΠ±ΡΠ΅ΡΡΠ½Π½ΠΎΠΌ Π΄Π΅ΡΠΈΡΠΈΡΠ΅ ΡΠΈΡΡΠ΅ΠΌΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ΠΎΠ² ΠΈ ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΠ΅ Π΄Π»Ρ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π·Π°ΠΌΠ΅ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π°ΠΌΠΈ
ΠΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°ΡΡΡΡ ΠΎΡΡΡΡΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΡΠ΅ΠΌΠΈΠ΅ΠΉ, ΠΏΠ΅ΡΠ΅Π½Π΅ΡΡΠΈΡ ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ
Objective: to develop prognostic criteria for frequent respiratory diseases in the first year of life in children with cerebral ischemia who had a cytomegalovirus infection in the neonatal period. Research methods: 73 children of the first year of life with cerebral ischemia, who underwent cytomegalovirus infection in the neonatal period, were deployed. All observed patients at the age of three months underwent a study of the population composition of peripheral blood T-lymphocytes using flow cytometry for the expression of membrane markers, taking into account the results on a Beckman Coulter Epics XL II laser flow cytometer. Typing of lymphocytes was carried out using monoclonal antibodies to differentiation clusters CD3+, CD3+CD69+, CD3+CD71+, CD3+CD95+ from Immunotech (France). The observation groups consisted of 30 children (41.1%) with frequent acute respiratory infections (4β5 episodes per year) in the first year of life and 43 people (58.9%) β children with no acute respiratory infectionβsepisodes in the first-year life. Results. From the set of studied T-lymphocytes, statistically significant for the prognosis of frequent acute respiratory infections in the first year of life in children with cerebral ischemia who underwent cytomegalovirus infection in the neonatal period were found: CD3+ CD71+, CD3+ CD95+. It was revealed that in children with a reduced level of CD3+ CD71+ and an increased level of CD3+ CD95+ in blood serum at the age of 3 months, frequent acute respiratory infections occurred in the first year of life.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°ΡΡ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΊΡΠΈΡΠ΅ΡΠΈΠΈ ΡΠ°ΡΡΡΡ
ΠΎΡΡΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΡΠ΅ΠΌΠΈΠ΅ΠΉ, ΠΏΠ΅ΡΠ΅Π½Π΅ΡΡΠΈΡ
ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 73 ΡΠ΅Π±Π΅Π½ΠΊΠ° ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π° ΠΆΠΈΠ·Π½ΠΈ Ρ ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΡΠ΅ΠΌΠΈΠ΅ΠΉ, ΠΏΠ΅ΡΠ΅Π½Π΅ΡΡΠΈΡ
ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ. ΠΡΠ΅ΠΌ Π½Π°Π±Π»ΡΠ΄Π°Π²ΡΠΈΠΌΡΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΡΡΠ΅Ρ
ΠΌΠ΅ΡΡΡΠ΅Π² ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΠΈ ΠΏΠΎ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Ρ ΡΡΠ΅ΡΠΎΠΌ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π½Π° ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΌ Π»Π°Π·Π΅ΡΠ½ΠΎΠΌ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠ΅ Beckman Coulter Epics XL II. Π’ΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» ΠΊ ΠΊΠ»Π°ΡΡΠ΅ΡΠ°ΠΌ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ Π‘D3+, CD3+ CD69+, CD3+ CD71+, CD3+ CD95+ ΡΠΈΡΠΌΡ Immunotech (Π€ΡΠ°Π½ΡΠΈΡ). ΠΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 30 Π΄Π΅ΡΠ΅ΠΉ (41,1%) Ρ ΡΠ°ΡΡΡΠΌΠΈ ΠΎΡΡΡΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΠΌΠΈ (ΠΠ Π) (4β5 ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² Π² Π³ΠΎΠ΄) Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ ΠΈ 43 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° (58,9%) β Π΄Π΅ΡΠΈ Ρ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² ΠΠ Π Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ (ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ°). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ· ΡΠΎΠ²ΠΎΠΊΡΠΏΠ½ΠΎΡΡΠΈ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ
Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π±ΡΠ»ΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΠ΅ Π΄Π»Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΡΠ°ΡΡΡΡ
ΠΎΡΡΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΡΠ΅ΠΌΠΈΠ΅ΠΉ, ΠΏΠ΅ΡΠ΅Π½Π΅ΡΡΠΈΡ
ΡΠΈΡΠΎΠΌΠ΅Π³Π°Π»ΠΎΠ²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ Π½ΠΎΠ²ΠΎΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ: CD3+ CD71+, CD3+ CD95+. ΠΡΡΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΠΏΠΎΠ½ΠΈΠΆΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ CD3+ CD71+ ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ CD3+ CD95+ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ 3-Ρ
ΠΌΠ΅ΡΡΡΠ΅Π² ΠΆΠΈΠ·Π½ΠΈ ΠΈΠΌΠ΅Π»ΠΈ ΠΌΠ΅ΡΡΠΎ ΡΠ°ΡΡΡΠ΅ ΠΎΡΡΡΡΠ΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Π½Π° ΠΏΠ΅ΡΠ²ΠΎΠΌ Π³ΠΎΠ΄Ρ ΠΆΠΈΠ·Π½ΠΈ