5 research outputs found
Amniotic Membrane Transplantation for Ocular Surface Reconstruction
The purpose of this study is to analyze the clinical experience and the effect of human
amniotic membrane transplantation on pterygium excision and bullous keratopathy.
From January 1999 to January 2001 at University Hospital »Sestre milosrdnice«
amniotic membrane transplantation was performed consecutively in 21 eyes: 11 eyes
with bullous keratopathy and 10 with recurrent pterygia. In the group with bullous
keratopathy epithelization took place in 19.6 days in 72.7% and the reduction of pain
was satisfactory. Recurrence rate in group with recurrent pterygia was 20%. Based on
the presented results it could be concluded that amniotic membrane transplantation
can be considered as an effective alternative for treating severe ocular surface diseases
and as an alternative for penetrating keratoplasty if there is a lack of graft
Amniotic Membrane Transplantation for Ocular Surface Reconstruction in Neurotrophic Corneal Ulcera
The purpose of this study is to analyze clinical experience about the effects of human
amniotic membrane transplantation in eyes with neurotrophic ulcers. In 11 eyes the application
of amniotic membrane was performed since January 1999 because of neurotrophic
ulcers. The follow up period was longer than 12 months: 19.76.0 months. The
average healing period after the surgery was 1.60.6 weeks. All corneas were fluorescein
negative even 12 months after operation. Visual acuity after the transplantation was
similar to the one before the surgery in 8 eyes. In 3 eyes the visual acuity after the surgery
was better than before. Amniotic membrane transplantation can be considered an
effective alternative for treating persistent epithelial defects such as neurotrophic ulcers.
It has some advantages over corneal transplantation: a relatively simple procedure, no
allograft rejection and it could be particularly beneficial in countries where cornea shortage
is apparent
Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems
We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 13− along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of 1H Nuclear Magnetic Resonance (NMR), 1H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry, Hybrid Replica Exchange Molecular Dynamics (REMD), and Microcrystal Electron Diffraction (MicroED) measurements are in line with 1 forming dimer [12]6−, to be in equilibrium with monomers 1(R)3− (relaxed) and 1(S)3− (squeezed). Through simultaneous line-shape analysis of 1H NMR data, kinetic and thermodynamic parameters characterizing these equilibria were quantified. Basket 1(R)3− includes anticancer drug mitoxantrone (MTO2+) in its pocket to give stable binary complex [MTO⊂1]− (Kd=2.1 μM) that can be precipitated in vitro with UV light or pH as stimuli. Both in vitro and in vivo studies showed that the basket is nontoxic, while at a higher proportion with respect to MTO it reduced its cytotoxicity in vitro. With well-characterized internal dynamics and dimerization, the ability to include mitoxantrone, and biocompatibility, the stage is set to develop sequestering agents from deep-cavity baskets