440 research outputs found
Haematological Alterations Due to Typhoid Fever in Enugu Urban- Nigeria
Some specific haematological changes that accompany chronic and severe typhoid fever were investigated in an endemic area of Enugu Urban-Nigeria. The results established that typhoid fever infections led to a statistically significant leucopenia (p < 0.05). In acute, chronic cases, leucopenia is accompanied with significant oligocythaemia, thrombocytopenia, anaemia and lowered haematocrit (p < 0.05). The importance of the results in the diagnosis and treatment of typhoid fever are discussed
Kinetic Study and Characterization of 1,4-β-Endoglucanase of Aspergillus niger ANL301
Submerged fermentation of Aspergillus niger ANL 301 in basal medium containing cellulose as sole carbon source, yielded crude extracellular proteins with 0.54 ± 0.02 units mg protein-1 of 1,4-β-endoglucanase activity. Partial purification by ammonium sulphate precipitation (80% saturation) and gel filtration on Sephadex 25-300 gave two active fractions of 1,4-β-endoglucanase, which exhibited close activity towards carboxymethyl-cellulose (CMC). The pH profile of the pooled enzyme fractions showed three activity peaks at pH 3.5, 5.5 and 7.0. The enzyme was most active at pH 5.5 and showed optimal activity at 50°C. Vmax of 4.4 ± 0.4 µmol min-1 mg protein-1 and Km of 12.5 ± 0.4 gL-1 was obtained with CMC for the enzyme. Different divalent metal ions and EDTA affected the enzyme activity at 2.0 mM concentrations in different ways. Mn2+ and Fe2+ exhibited 253.4 and 24.0% stimulatory effects, respectively on the enzyme activity. Mg2+, Ca2+, Cu2+, and Zn2+ inhibited the enzyme by between 22.3 and 29.4%, whereas 75.0 and 71.3% inhibition were obtained with Hg2+ and EDTA, respectively. Manganese ion showed an exceptional activation of the 1,4-β-endoglucanase. The organism produced two types of 1,4-β-endoglucanase with different molecular weights
Narrowing the Expectation Gap in Auditing: The Role of the Auditing Profession
The study sought to ascertain the role of the auditing profession in narrowing the audit expectation gap. Selfadministered
questionnaires were used in the study. The data generated from the responses of the subjects were
analyzed using descriptive and statistical analysis through the computer (SPSS 16.0).
The result shows that the public is ignorant of the duties of the auditor and this lack of knowledge is responsible for
unreasonable expectations of the public from auditors.
Based on the findings and conclusion, it was recommended that the public need more education on the duties and
responsibilities of the auditor, the standard auditor’s report should be expanded to include disclaimer clauses clearly
showing that it is not a certificate or guarantee of the financial soundness of the auditee, it should be clearly stated in
the audit report that the auditor is not the Compliance Officer of the audited company and that the auditors report
should add that the opinion expressed by the auditor should not be construed to mean a guarantee of accuracy of the
financial statement
Xylanase production by Aspergillus niger ANL 301 using agro - wastes
Xylanase production by wild-type Aspergillus niger ANL301, newly isolated from wood-waste, was
monitored at 24 h intervals for a period 168 h in media containing different carbon sources. The carbon
sources were oat-spelt xylan (Fluka) and three agro-wastes (sawdust, sugarcane pulp and wheat bran).
Highest xylanase activity of 6.47 units/mL was obtained at 96 h in media containing wheat bran as sole
carbon source. Maximum activity value for the media containing sugarcane pulp was 0.95 units/mL
obtained also at 96 h. Sawdust and oat spelt xylan gave the peak enzyme activities of 0.65 and 0.80
units/mL respectively at 120 h. High protein yield was obtained in media containing the agro-wastes,
with wheat bran giving the highest value of 1.14 mg/mL at 96 h. The maximum specific xylanase
activities were 3.86, 3.37, 5.69, and 9.36 units/ mg protein for sawdust, sugarcane pulp, wheat bran and
oat spelt xylan, respectively. Out of the three agro-wastes used in this study, wheat bran holds greatest
promise for low cost production of the xylanase enzyme
Agro-waste: a potential fermentation substrate for Penicillium chrysogenum
Common agro-wastes found in Lagos, Nigeria (cassava shavings, corncob, sawdust, and sugarcane
pulp) were compared with glucose and lactose as fermentation substrates for Penicillium chrysogenum
PCL501. Cassava shavings significantly (P<0.001) produced the highest amount of mycelia weight (0.43 ±
0.02 mg/ml) than all the other substrates. This was followed by corncob with peak mycelia weight of 0.33 ±
0.02 mg/ml. Peak mycelia weight of 0.27 ± 0.01 mg/ml was equally obtained with glucose and sugarcane pulp
whereas lactose gave a slightly lower peak of 0.25 ± 0.01 mg/ml. Sawdust gave the least mycelia weight of
0.13 ± 0.01 mg/ml. Total sugar content of all the culture media steadily decreased as fungal growth progressed
indicating that the organism utilized carbohydrates for growth and mycelia formation. Cultures containing
cassava shavings and sawdust gave high protein peaks of 0.84 ± 0.05 and 0.65 ± 0.03 mg/ml respectively.
Cultures containing corncob, glucose, lactose and sugarcane pulp yielded lower protein peaks of 0.37 ± 0.02,
0.30 ± 0.02, 0.24 ± 0.02 and 0.18 ± 0.01 mg/ml respectively. The results suggest that cassava shavings,
corncob and sugarcane pulp could serve as cheap fermentation substrates for the growth of the fungus. Of all
the substrates investigated, cassava shavings have the best potential to serve as substrate for fermentation by
Penicillium chrysogenum PCL501.
© 2009 International Formulae Group. All rights reserved
Effect of carbon sources on cellulase (EC 3. 2. 1. 4) production by Penicillium chrysogenum PCL501
The effects of glucose, crystalline cellulose and sawdust of Mitragyna cilata on the growth and cellulase
production, inferred from cellulase (EC 3. 2. 1. 4) activity, of Penicillium chrysogenum PCL501 was
determined. Glucose-containing media gave the highest mycelia weight of 1.78 mg mL-1 in 120 h of
incubation. This is about 3.5 – 4.5 times the maximum weights of 0.51 and 0.40 mg mL-1 respectively
obtained from the cultures containing cellulose and sawdust. The cultures containing crystalline
cellulose and sawdust produced extracellular protein with cellulase (EC 3. 2. 1. 4) activity whereas
glucose-containing cultures yielded very low protein and no significant cellulase activity. Maximum
protein content of 0.02, 0.13 and 0.46 mg mL-1 respectively were obtained from the cultures containing
glucose, cellulose and sawdust. Peak cellulase activity values of 100.0 and 92.2 Units L-1 respectively
were obtained for the cultures containing cellulose and sawdust. There is a correlation between the
protein released and cellulase activity of the culture filtrates. P. chrysogenum PCL501 produces
extracellular proteins with significant cellulase activity in media containing cellulose and sawdust but
not in glucose-containing medium. Sawdust is indicated as a good inducer of cellulase activity in the
organism. The waste cellulosic material can be used as low-cost carbon source for commercial
cellulase production
Communication: An Effective Tool for Employee Performance in Lever Brothers Nigeria PLC.
Descriptive survey research design was adopted for the study and the total population of the study were 142 respondents. The simple random sampling technique was used to select the participants. Data gathered were analysed using Pearson Product Moment Correlation (PPMC). The result of the analysis revealed that there was significant relationship between communication and employee performance. It was therefore concluded that organizations should endeavour to make effective communication an essential strategy in achieving high employee performance. Keywords: Communication Completeness, Communication Courtesy, Employee Performance
Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes
Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was
monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oatspelt
xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1
was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a
peak value of 1.39 Units mL-1 at 144 h. Sawdust and xylan gave a peak xylanase activity of 1.35 and 0.79
Units mL-1 respectively at 120 h. Maximum protein released in xylan-containing media was 0.38 mg mL-1.
Higher protein yield was obtained in media containing the agro-wastes, with wheat bran giving the
highest value of 1.14 mg mL-1. The maximum specific xylanase activities were 2.59, 8.52, 16.06, and 9.36
Units mg Protein -1 for sawdust, sugarcane pulp, wheat bran and xylan respectively. Out of the three
agro-wastes used in this study, wheat bran holds the greatest promise for cost-effective production of
the xylanase enzyme. The carbon source is the highest inducer of the enzyme in the fungus
Evaluation of the Side Effects of Cisplatin Drug in a Nephrotoxicity Model of Wistar Rats
This study critically assessed the observable side effects of cisplatin (anti-tumor) drug in a Portulaca oleracea treated nephrotoxicized wistar rats. Twenty four albino female rats were grouped into four-groups A, B, C, & D. Group A served as the control group; group C and D were all nephrotoxicized using 2ml/kg cisplatin and treated with 400mg/kg and 800mg/kg doses of Portulaca oleracea methanolic extract respectively. Group B were nephrotoxicized but remained untreated. Significant Increase in serum creatinine (P<0.05) in group B rats showed presence of nephrotoxicity and results further showed recovery (significant decrease at P<0.05) in serum creatinine level of groups C &D rats due to the administration of portulaca oleracea. Physically observable features were examined in the rats at the 4th & 7th day of drug administration which showed the presence of side effects (ototoxicity, loss of appetite, black stool, dizziness, fever, etc) which were seen to be increasing with prolonged administration. This research posits seemingly untreatable side effect of cisplatin chemotherapy which increases with increased chemotherapy duration and decreased side effects in the combination of portulaca oleracea in cisplatin chemotherapy. Keywords: chemotherapy, cisplatin and nephrotoxicity
Plant Waste Hydrolysis by Extracellular Enzymes of Aspergillus niger and Penicillium chrysogenum: Effect of Ammonia Pretreatment
Aspergillus niger (ANL301) and Penicillium chrysogenum (PCL 501) cultured in basal media with cellulose as sole
carbon source yielded extracellular enzymes which partially hydrolyzed sawdust and sugarcane pulp into simple
sugars. Pre-treatment of sawdust by ammonium hydroxide steeping increased the yield of simple sugars. The reducing
sugars released from the pretreated sawdust by the crude enzymes of A. niger (ANL301) and P. chrysogenum
(PCL 501) were 3.58% and 7.02% of the total hydrolysable sugars respectively. This is in contrast to the 0.92% and
1.02% of the total hydrolysable sugars released respectively by the enzymes of A. niger (ANL301) and P. chrysogenum
(PCL 501) from the non-pretreated sawdust. Enzymatic hydrolysis of sugarcane pulp by the crude enzymes
was not significantly affected by ammonia pre-treatment. Reducing sugars released from non-pretreated sugarcane
pulp by the crude enzymes of A. niger (ANL301) and P. chrysogenum (PCL 501) were respectively 4.17% and
5.08% of the total hydrolysable sugars
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