381 research outputs found

    Enhanced electron field emission properties by tuning the microstructure of ultrananocrystalline diamond film

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    [[abstract]]Synthesis of microcrystalline-ultrananocrystalline compositediamond (MCD-UNCD) films, which exhibit marvelous electron field emission (EFE) properties, was reported. The EFE of MCD-UNCD compositediamondfilm can be turned on at a low field as 6.5 V/μm and attain large EFE current density about 1.0 mA/cm2 at 30 V/μm applied field, which is better than the EFE behavior of the nondoped planar diamondfilms ever reported. The MCD-UNCD films were grown by a two-step microwave plasma enhanced chemical vapor deposition (MPECVD) process, including forming an UNCD layer in CH4/Ar plasma that contains no extra H2, followed by growingMCD layer using CH4/H2/Ar plasma that contains large proportion of H2. Microstructure examinations using high resolution transmission electron microscopy shows that the secondary MPECVD process modifies the granular structure of the UNCD layer, instead of forming a large grain diamond layer on top of UNCDfilms. The MCD-UNCD compositediamondfilms consist of numerous ultrasmall grains (∼5 nm in size), surrounding large grains about hundreds of nanometer in size. Moreover, there exist abundant nanographites in the interfacial region between the grains that were presumed to form interconnected channels for electron transport, resulting in superior EFE properties for MCD-UNCD compositefilms.[[incitationindex]]SCI[[booktype]]電子版[[booktype]]紙

    VI-Band Follow-Up Observations of Ultra-Long-Period Cepheid Candidates in M31

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    The ultra-long period Cepheids (ULPCs) are classical Cepheids with pulsation periods exceeding 80\approx 80 days. The intrinsic brightness of ULPCs are ~1 to ~3 mag brighter than their shorter period counterparts. This makes them attractive in future distance scale work to derive distances beyond the limit set by the shorter period Cepheids. We have initiated a program to search for ULPCs in M31, using the single-band data taken from the Palomar Transient Factory, and identified eight possible candidates. In this work, we presented the VI-band follow-up observations of these eight candidates. Based on our VI-band light curves of these candidates and their locations in the color-magnitude diagram and the Period-Wesenheit diagram, we verify two candidates as being truly ULPCs. The six other candidates are most likely other kinds of long-period variables. With the two confirmed M31 ULPCs, we tested the applicability of ULPCs in distance scale work by deriving the distance modulus of M31. It was found to be μM31,ULPC=24.30±0.76\mu_{M31,ULPC}=24.30\pm0.76 mag. The large error in the derived distance modulus, together with the large intrinsic dispersion of the Period-Wesenheit (PW) relation and the small number of ULPCs in a given host galaxy, means that the question of the suitability of ULPCs as standard candles is still open. Further work is needed to enlarge the sample of calibrating ULPCs and reduce the intrinsic dispersion of the PW relation before re-considering ULPCs as suitable distance indicators.Comment: 13 pages, with 14 Figures and 4 Tables (one online table). AJ accepte

    ERP Post-Implementation Learning, ERP Usage And Individual Performance Impact

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    In recent years, an increasing number of companies that have implemented ERP systems have expressed disappointment over a failure to reach anticipated goals. A major reason for this failure is the inefficient use of the ERP system by employees. Therefore, the critical issue is how users can most effectively take advantage of an ERP system. Post-implementation learning plays an important role in facilitating ERP usage and thus promotes individual performance. Particularly, the integrated and sophistic natures of ERP systems force users to learn continuously after ERP implementation. This study employed a survey method to examine the perceptions of a dataset of 659 ERP users. We found that ERP usage facilitates individual performance, including individual productivity, customer satisfaction and management control, and post-implementation learning contributes to all three types of ERP usage, including decision support, work integration and customer service. Our findings can provide academics and practitioners with knowledge of how to improve ERP usage and ensure individual performance impacts

    Maintaining the structural integrity of thebamboo mosaic virus 3′ untranslated region isnecessary for retaining the catalytic constant forminus-strand RNA synthesis

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    Background: Bamboo mosaic virus (BaMV) and the Potato virus X (PVX) are members of the genus Potexvirus andhave a single-stranded positive-sense RNA genome. The 3′-untranslated region (UTR) of the BaMV RNA genomewas mapped structurally into ABC (a cloverleaf-like), D (a stem-loop), and E (pseudoknot) domains. The BaMVreplicase complex that was isolated from the infected plants was able to recognize the 3′ UTR of PVX RNA toinitiate minus-strand RNA synthesis in vitro.Results: To investigate whether the 3′ UTR of PVX RNA is also compatible with BaMV replicase in vivo, weconstructed chimera mutants using a BaMV backbone containing the PVX 3′ UTR, which was inserted in or used toreplace the various domains in the 3′ UTR of BaMV. None of the mutants, except for the mutant with the PVX3′ UTR inserted upstream of the BaMV 3′ UTR, exhibited a detectable accumulation of viral RNA in Nicotianabenthamiana plants. The in vitro BaMV RdRp replication assay demonstrated that the RNA products were generatedby the short RNA transcripts, which were derived from the chimera mutants to various extents. Furthermore, theVmax/KM of the BaMV 3′ UTR (rABCDE) was approximately three fold higher than rABCP, rP, and rDE in minus-strandRNA synthesis. These mutants failed to accumulate viral products in protoplasts and plants, but were adequatelyreplicated in vitro.Conclusions: Among the various studied BaMV/PVX chimera mutants, the BaMV-S/PABCDE that containednon-interrupted BaMV 3′ UTR was the only mutant that exhibited a wild-type level of viral product accumulation inprotoplasts and plants. These results indicate that the continuity of the domains in the 3′ UTR of BaMV RNA wasnot interrupted and the domains were not replaced with the 3′ UTR of PVX RNA in vivo

    Logic-based Temporal Inferences in Natural Language

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    This paper concerns temporal inferences in natural language. The concept of time is crucially important because of its highly frequent use in utterances. However, time can appear in numerous forms, for example, tense or time adverbials. It also may be implicitly specified by other events associating with some temporal conjunctions, such as when, before, after, etc. A logic-based approach is adopted to represent temporal information. Situations and time adverbials can be formally represented. Lexical knowledge about time and implications of situations also expressed in terms of logical clauses. Then we propose a temporal reasoning model. Various basic types of questions such as "When did event X happen?", "How long did event X last for?", "Did event X happen at time expression T?", etc. can be interpreted into temporal queries. Finally, we also use the same framework of logic to evaluate temporal queries, carry out computation and do the further deduction

    Parametric modeling of cellular state transitions as measured with flow cytometry

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    <p>Abstract</p> <p>Background</p> <p>Gradual or sudden transitions among different states as exhibited by cell populations in a biological sample under particular conditions or stimuli can be detected and profiled by flow cytometric time course data. Often such temporal profiles contain features due to transient states that present unique modeling challenges. These could range from asymmetric non-Gaussian distributions to outliers and tail subpopulations, which need to be modeled with precision and rigor.</p> <p>Results</p> <p>To ensure precision and rigor, we propose a parametric modeling framework StateProfiler based on finite mixtures of skew <it>t</it>-Normal distributions that are robust against non-Gaussian features caused by asymmetry and outliers in data. Further, we present in StateProfiler a new greedy EM algorithm for fast and optimal model selection. The parsimonious approach of our greedy algorithm allows us to detect the genuine dynamic variation in the key features as and when they appear in time course data. We also present a procedure to construct a well-fitted profile by merging any redundant model components in a way that minimizes change in entropy of the resulting model. This allows precise profiling of unusually shaped distributions and less well-separated features that may appear due to cellular heterogeneity even within clonal populations.</p> <p>Conclusions</p> <p>By modeling flow cytometric data measured over time course and marker space with StateProfiler, specific parametric characteristics of cellular states can be identified. The parameters are then tested statistically for learning global and local patterns of spatio-temporal change. We applied StateProfiler to identify the temporal features of yeast cell cycle progression based on knockout of S-phase triggering cyclins Clb5 and Clb6, and then compared the S-phase delay phenotypes due to differential regulation of the two cyclins. We also used StateProfiler to construct the temporal profile of clonal divergence underlying lineage selection in mammalian hematopoietic progenitor cells.</p

    Universal scaling of extinction time in stochastic evolutionary dynamics

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    Evolutionary dynamics is well captured by the replicator equations when the population is infinite and well-mixed. However, the extinction dynamics is modified with finite and structured populations. Experiments on the non-transitive ecosystem containing three populations of bacteria found that the ecological stability sensitively depends on the spatial structure of the populations. Based on the Reference–Gamble–Birth algorithm, we use agent-based Monte Carlo simulations to investigate the extinction dynamics in the rock-paper-scissors ecosystem with finite and structured populations. On the fully-connected network, the extinction time in stable and unstable regimes falls into two universal functions when plotted with the rescaled variables. On the two dimensional grid, the spatial structure changes the transition boundary between stable and unstable regimes but doesn’t change its extinction trend. The finding of universal scaling in extinction dynamics is unexpected, and may provide a powerful method to classify different evolutionary dynamics into universal classes
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