Evaluation of Bioaccumulation and Toxicity of Bisphenol-A and Phthalates on Earthworm and Nitrifying Bacteria from Soil Collected in Waste Management Landfill, Kaduna State, Nigeria

Additives such as bisphenol-A (BPA) and phthalates are added to plastics during production processes in order to improve their properties. Since they are not covalently bound but simply mixed with the plastic polymer. They disperse easily in the environment, especially when plastic products are degraded into microplatics. Hence, the objective of this paper was to evaluate bioaccumulation and toxicity of bisphenol-A and phthalates on  earthworm (Apporrectoda ionga) and nitrifying bacteria from soil collected in Waste management landfill, Kaduna Sate, Nigeria using standard techniques. Data obtained shows that Bisphenol A, Diethytlphalate, Dimethylphthalate and Diethylhexylphalate were detected in the plastic enriched compositing soil while only bisphenol A was detected in the control soil sample. The physico-chemical analysis of the composted soil and the control had electrical conductivity 254.00, 61.00us/cm, chloride 66.15, 16.00mg/kg, potassium 171.5, 4.25mg/kg, nickel 1.00, 0.25mg/kg, Iron 17.40, 3.11mg/kg, Zinc 2.90, 0.67mg/kg, moisture 5.32, 7.21% total organic carbon 5.26, 0.71% and total nitrogen 0.52, 0.07% respectively. The bacteriological analysis for composted soil and control soil growth ranging from 4.0 x 103+0.12 to 3.5x103+104cfu/g and 3.2x103+0.4 to 2.8x103+0.10 respectively. There was significant difference (p<0.05) in the bacteria counts from the control soil sample. The toxicity analysis revealed higher percentage utilization of nitrite with LC50 values of 25.04, 23.93, 15.9 and 13.39 and higher bacteria inhibition with EC50 values of 52.00, 81.72, 111.31 and 123.13. The results suggest that autotrophic transformation by nitrifying bacteria which enhances soil fertility may be hindered in an ecosystem polluted with these plasticizers as nitrification process will reduced. Percentage survival rate of earth worms decreased with increase in plastic concentration (75.47-20.93%)

Does Vitamin D Deficiency Cause Hypertension? Current Evidence from Clinical Studies and Potential Mechanisms

Vitamin D deficiency is widely prevalent across all ages, races, geographical regions, and socioeconomic strata. In addition to its important role in skeletal development and calcium homeostasis, several recent studies suggest its association with diabetes, hypertension, cardiovascular disease, certain types of malignancy, and immunologic dysfunction. Here, we review the current evidence regarding an association between vitamin D deficiency and hypertension in clinical and epidemiological studies. We also look into plausible biological explanations for such an association with the renin-angiotensin-aldosterone system and insulin resistance playing potential roles. Taken together, it appears that more studies in more homogeneous study populations are needed before a firm conclusion can be reached as to whether vitamin D deficiency causes or aggravates hypertension and whether vitamin D supplementation is safe and exerts cardioprotective effects. The potential problems with bias and confounding factors present in previous epidemiological studies may be overcome or minimized by well designed randomized controlled trials in the future

Understanding the impact of antenatal care policies in Georgia (USA) and Scotland (UK): a textual synthesis. [Preprint]

This study aims to: 1) understand the role of policy in maternal health outcomes; and 2) establish any differences or similarities between health systems, providing benchmarks for future maternal and infant care policies in Georgia and Scotland. Guided by JBI methodology, a textual review of policies and public health interventions that have influenced the antenatal care process in both health systems was conducted. Inclusion criteria for this review were classified using the "PCC" mnemonic: Population (pregnant women and mothers), Concept (policies and strategies that support prenatal and maternal health) and Context (relevant to Scotland and Georgia). Published primary and secondary research, and grey literature (guidelines, reports, and legislation from authoritative sources) were included. Overall, 60 sources contributed to the report on maternal health system topics. Findings of the textual synthesis presented a regionalized system of maternity care led by physician-provided care models in Georgia compared to the nationalized health system in Scotland with an extended scope for midwife-led care models. On a secondary, organizational level, Scotland also widely operates on protocolized, standardized care informed by clinical guidelines such as NICE. The Georgia health systems also follow national guidelines for care, but extent of standardization may vary based on a mixed system of private and public insurance coverage. This is the first study to comprehensively examine maternal health policies in the distinct contexts of Georgia and Scotland, shedding light on the diverse approaches within their respective healthcare systems. These observed variations stem from historical, cultural, and policy contexts unique to each region. As the United States continue to prioritize maternal and child health through public health initiatives, our findings feature crucial considerations for maternal antenatal care policies. Specifically, there is a discernible need to increase access to antenatal care and invest in the maternity care provider workforce, revealing opportunities for targeted improvements in support of maternal health

Incident Diabetes Complications among Women with type 1 diabetes based on Parity

Objectives To assess risk factors and incidence of diabetes complications in women with type 1 diabetes (T1D) based on parity. Research design/methods Data were collected from women (16–40 years old) in the T1D Exchange completing pregnancy/childbirth questionnaires during 2011–2013 and 2016–2018. Incidence of risk factors and diabetes complications were compared between women with a first pregnancy at/within 1-year of enrollment (n = 28) and never pregnant women by year 5 (n = 469). Results There was a trend for lower HbA1c (adjusted p = .14) and higher rates of overweight/obesity, triglyceride/HDL > 2, log (triglyercide/HDL), and hypertension among parous women compared with nulliparous women. There were no significant differences in rates of advanced nephropathy, albuminuria or cardiovascular disease. Conclusions Four-5 years after delivery, parous women with T1D tended to have lower HbA1c levels despite higher body mass indices and more frequent adverse lipid profiles and hypertension compared with nulliparous women. Further studies based on these trends are warranted

Prevalence and cardiometabolic associations of the glucocorticoid receptor gene polymorphisms N363S and Bcl1 in obese and non-obese black and white Mississippians

AbstrAct ObJEctIVE: Polymorphisms (sNP) in the glucocorticoid receptor (Gr) gene can alter sensitivity to glucocorticoids. Previous studies of the N363s and BclI sNP in the Gr gene have shown a metabolic syndrome phenotype in mostly non-African populations. the obesity phenotype of African Americans (AA) seems to be more severe than that of caucasians. DEsIGN: We aimed to assess the prevalence of N363s and BclI in obese and non-obese caucasian (n=26) and African (n=23) Mississippians (age: 23-63 years) to investigate associations with body composition (body mass index/bMI, waist-to-hip ratio), metabolic parameters (salivary cortisol, fasting glucose and insulin, hemoglobin A1c, fructosamine, HOMA-Ir index), and psychological stress perception (blood pressure/bP, perceived stress scale/Pss). rEsULts: All subjects were homozygous for wildtype N363N. BclI polymorphism genotype frequencies among the 23 AA were: homozygous cc (57%), GG (4%), and heterozygous cG (39%), and among the 26 white women: homozygous cc (35%), GG (19%), and heterozygous cG (46%). Linear and logistic regression analyses including a parsimonious model identified bMI as a statistically significant parameter between the two ethnic groups (bMI was 3.13 kg/m 2 higher in AA). Within the AA group, bMI, waist-to-hip ratio, log (HOMA-Ir), Pss scores, bP, and hyperlipidemia showed no statistically significant relationships for the BclI polymorphism. Pss scores were 15.2 for AA vs. 14.7 for white women (normal mean: 14.7 vs. 12.8). cONcLUsION: black Mississippians HORMONES 2012, 11(2):166-17

Ectopic Cushing' syndrome caused by a neuroendocrine carcinoma of the mesentery

BACKGROUND: ACTH overproduction within the pituitary gland or ectopically leads to hypercortisolism. Here, we report the first case of Cushing' syndrome caused by an ectopic ACTH-secreting neuroendocrine carcinoma of the mesentery. Moreover, diagnostic procedures and pitfalls associated with ectopic ACTH-secreting tumors are demonstrated and discussed. CASE PRESENTATION: A 41 year-old man presented with clinical features and biochemical tests suggestive of ectopic Cushing's syndrome. First, subtotal thyroidectomy was performed without remission of hypercortisolism, because an octreotide scan showed increased activity in the left thyroid gland and an ultrasound revealed nodules in both thyroid lobes one of which was autonomous. In addition, the patient had a 3 mm hypoenhancing lesion of the neurohypophysis and a 1 cm large adrenal tumor. Surgical removal of the pituitary lesion within the posterior lobe did not improve hypercortisolism and we continued to treat the patient with metyrapone to block cortisol production. At 18-months follow-up from initial presentation, we detected an ACTH-producing neuroendocrine carcinoma of the mesentery by using a combination of octreotide scan, computed tomography scan, and positron emission tomography. Intraoperatively, use of a gamma probe after administration of radiolabeled (111)In-pentetreotide helped identify the mesenteric neuroendocrine tumor. After removal of this carcinoma, the patient improved clinically. Laboratory testing confirmed remission of hypercortisolism. An octreotide scan 7 months after surgery showed normal results. CONCLUSION: This case underscores the diagnostic challenge in identifying an ectopic ACTH-producing tumor and the pluripotency of cells, in this case of mesenteric cells that can start producing and secreting ACTH. It thereby helps elucidate the pathogenesis of neuroendocrine tumors. This case also suggests that patients with ectopic Cushing's syndrome and an octreotide scan positive in atypical locations may benefit from explorative radioguided surgery using (111)In-pentetreotide and a gamma probe

Somatostatin and dopamine receptors as targets for medical treatment of Cushing's Syndrome

Somatostatin (SS) and dopamine (DA) receptors are widely expressed in neuroendocrine tumours that cause Cushing's Syndrome (CS). Increasing knowledge of specific subtype expression within these tumours and the ability to target these receptor subtypes with high-affinity compounds, has driven the search for new SS- or DA-based medical therapies for the various forms of CS. In Cushing's disease, corticotroph adenomas mainly express dopamine receptor subtype 2 (D2) and somatostatin receptor subtype 5 (sst5), whereas sst2is expressed at lower levels. Activation of these receptors can inhibit ACTH-release in primary cultured corticotroph adenomas and compounds that target either sst5(pasireotide, or SOM230) or D2(cabergoline) have shown significant efficacy in subsets of patients in recent clinical studies. Combination therapy, either by administration of both types of compounds separately or by treatment with novel somatostatin-dopamine chimeric molecules (e.g. BIM-23A760), appears to be a promising approach in this respect. In selected cases of Ectopic ACTH-producing Syndrome (EAS), the sst2-preferring compound octreotide is able to reduce cortisol levels effectively. A recent study showed that D2receptors are also significantly expressed in the majority of EAS and that cabergoline may decrease cortisol levels in subsets of these patients. In both normal adrenal tissue as well as in adrenal adenomas and carcinomas that cause CS, sst and DA receptor expression has been demonstrated. Although selected cases of adrenal CS may benefit from sst or DA-targeted treatment, its total contribution to the treatment of these patients is likely to be low as surgery is effective in most cases

Full Length Research Paper - Stable gene transformation in cowpea (Vigna unguiculata L. walp.) using particle gun method

We investigated the possibility of transforming and obtaining transgenic cowpea (Vigna unguiculata L Walp) plants using the particle bombardment process. Meristematic explants that could give rise to whole fertile plants were used in transformation experiments with reporter and selectable marker genes driven by a 35S CaMV promoter. Conditions for optimal delivery of DNA to explants were established based on transient gus expression assays two days after bombardment. The size of microcarriers, microflight distance and helium pressure significantly affected transient expression of reporter genes. A total of 1692 explants were bombarded with DNA-coated particles and placed on 3 mg/l bialaphos selective medium. Only 12 regenerated shoots produced seeds eventually, and all were Gus negative even though 7 gave positive PCR signals with the bar primer. Eight out of 1400 seeds from To plants were GUS positive. DNA from eight of the GUS positive seedlings were amplified with both the gus and bar primers in PCR analysis but only two gave a positive Southern signal. Only two of the 3557 T2 seedlings obtained were GUS positive. However, 3 seedlings survived Basta spray. The two GUS positive and 3 Basta surviving seedlings gave positive Southern hybridisation signals. Twelve T3 seedlings from these were GUS positive and also gave positive Southern hybridisation signals. The positive reaction of T1, T2 and T3 seedlings under Southern analysis confirms the stable integration of introduced genes and the transfer of such genes to progenies. However, the level of expression of introduced genes in cowpea cells is very low and this accounted for the high mortality rate of progenies under Basta spray