107 research outputs found
Hepatotoxic Effects of Sera from Patients with Fulminant Hepatitis B on Isolated Rat Hepatocytes in Culture
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Einfluß verschiedener Methoden zur Blutgewinnung auf Enzym-Aktivitäten im Serum kleiner Laboratoriumstiere
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Biochemistry and functional aspects of human glandular kallikreins
Human urinary kallikrein was purified by gel filtration on Sephacryl S-200 and affinity chromatography on aprotinin-Sepharose, followed by ion exchange chromatography on DEAE-Sepharose. In dodecylsulfate gel electrophoresis two protein bands with molecular weights of 41,000 and 34,000 were separated. The amino acid composition and the carbohydrate content of the kallikrein preparation were determined; isoleucine was identified as the only aminoterminal amino acid. The bimolecular velocity constant for the inhibition by diisopropyl fluorophosphate was determined as 9±2 l mol–1 min–1. The hydrolysis of a number of substrates was investigated and AcPheArgOEt was found to be the most sensitive substrate for human urinary kallikrein. Using this substrate an assay method for kallikrein in human urine was developed.
It was shown by radioimmunoassay that pig pancreatic kallikrein can be absorbed in the rat intestinal tract. Furthermore, in dogs the renal excretion of glandular kallikrein from blood was demonstrated by radioimmunological methods
Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma
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Biological, Analytical and Experimental Components of Variance in a Long-Term Study of Plasma Constituents in Rat
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Untersuchungen zur Vergleichbarkeit der Ergebnisse unterschiedlicher Methoden zur radioimmunologischen Digoxin-Bestimmung. Ein Beitrag zur Qualitätskontrolle
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