Evaluation of Crude Oil Contaminated Soils in Parts of Bayelsa State, Nigeria

The study evaluated crude oil contaminated soils in both central and eastern parts of Bayelsa State, Nigeria. Soil samples were collected by adopting a rectangular grid and drilling with the aid of a hand auger at the center (highest point of concentration) of the rectangle for proper representation of samples. Soil samples were collected at varying depths of top/bottom, 1m, 2m, and 3m, and were analyzed at Anal Concept Laboratory, Port Harcourt using standard methods as applicable. Results show acidity trend in all locations including control site with pH ranging from 3.6 to 4.9, except at ETE where a pH of 8.0 was obtained. Even this figure is slightly above the stipulated range of 6.0 to 7.0 pH for healthy plants growth.  Mean values of EC range from 11us/cm to 59us/cm, whilst EC measurement at control location recorded very high value of 759us/cm showing negative influence of spill on soil nutrients. Seasonal variation was also noticed as mean EC levels dropped tremendously from 5.9 to 41.0us/cm during the wet season. Although HCO32- and CO3- are not very important nutrients for soil because they react with soil calcium to form Ca(HCO3)2 or CaCO3., they act as organic matter stabilizers in calcareous soils.  The result showed that both parameters had reduced concentrations for dry and wet seasons in all locations compared with control results of (4.1 and 2.0 mg/l) and (2.7 and 1.5 mg/l) for dry and wet seasons. SO42- (mg/kg) values ranged from 2.0 to 14.0 mg/l in dry period and reduced further to 1.0 – 3.0mg/l in the wet season. Measurement in the control obtained a high level of 200 and 129mg/l for dry and wet season respectively. Chloride values ranged from 4.0 – 24mg/l for dry period and 2.0 – 9.0mg/l for wet period.  Whilst control station recorded 31.0 and 62.0mg/l for wet and dry seasons respectively, depicting a drastic reduction in spill impacted areas. For organics, PAH recorded peak mean figure of 5.87mg/l at IKM top soil and a minimum mean value of 0.01mg/l at OTU top soil. PAH increased in concentration at different depths in all locations depicting a redistribution and infiltration of petroleum in the soil. On the contrary, OTU and IKM had very low values of PAH indicating low PAH concentration. Similarly, TPH peak for dry season was obtained at IkM T/B (539mg/l) followed by Orm 1-3 (318.26mg/l). But low levels were observed at ETE 1m and Oru 1-3m. The wet season had huge increase in concentration levels as maximum value of 2094.4mg/l was obtained at IKM T/B depicting crude redistribution aided by flood impact. High trend continued almost in all locations except for ETE 1 and ORU 1-3m. Cation such as Calcium showed increased concentration in the wet season at all locations, while, Na+, Mg2+ and K+ experienced drastic reduction during the wet season depicting dissolution on contact with water. For heavy metals, Arsenic recorded very low value of <0.01mg/l across the entire study area, Nickel had not much seasonal variations except for ETE 1 and ORM 1-3m where slight concentration levels were observed. Lead and Vanadium also behaved in similar manner as Ni2+ because increased levels were only encountered at ETE 1, ORM 1-3m and OTU 0-0.3m. On the contrary, Cadmium increased in levels at all locations in both seasons except for in ETE 1. Results from the control station also showed high values of 8.43mg/l and 4.57mg/l for Nickel and Lead respectively indicating natural existence of heavy metals in the earth which can be enhanced by anthropogenic activities. Deductions from the soil profile show predominance of sandstone across the study area indicative of high infiltration most likely the reason for the high level of crude oil observed in spill impacted samples. Keywords: soils, contamination, crude oil, impacted area DOI: 10.7176/JEES/13-5-05 Publication date:July 31st 202

A Hybrid Analytic Network Process and Artificial Neural Network (ANP-ANN) model for urban Earthquake vulnerability assessment

© 2018 by the authors. Vulnerability assessment is one of the prerequisites for risk analysis in disaster management. Vulnerability to earthquakes, especially in urban areas, has increased over the years due to the presence of complex urban structures and rapid development. Urban vulnerability is a result of human behavior which describes the extent of susceptibility or resilience of social, economic, and physical assets to natural disasters. The main aim of this paper is to develop a new hybrid framework using Analytic Network Process (ANP) and Artificial Neural Network (ANN) models for constructing a composite social, economic, environmental, and physical vulnerability index. This index was then applied to Tabriz City, which is a seismic-prone province in the northwestern part of Iran with recurring devastating earthquakes and consequent heavy casualties and damages. A Geographical Information Systems (GIS) analysis was used to identify and evaluate quantitative vulnerability indicators for generating an earthquake vulnerability map. The classified and standardized indicators were subsequently weighed and ranked using an ANP model to construct the training database. Then, standardized maps coupled with the training site maps were presented as input to aMultilayer Perceptron (MLP) neural network for producing an Earthquake VulnerabilityMap (EVM). Finally, an EVMwas produced for Tabriz City and the level of vulnerability in various zones was obtained. South and southeast regions of Tabriz City indicate low to moderate vulnerability, while some zones of the northeastern tract are under critical vulnerability conditions. Furthermore, the impact of the vulnerability of Tabriz City on population during an earthquake was included in this analysis for risk estimation. A comparison of the result produced by EVM and the Population Vulnerability (PV) of Tabriz City corroborated the validity of the results obtained by ANP-ANN. The findings of this paper are useful for decision-makers and government authorities to obtain a better knowledge of a city's vulnerability dimensions, and to adopt preparedness strategies in the future for Tabriz City. The developed hybrid framework of ANP and ANN Models can easily be replicated and applied to other urban regions around the world for sustainability and environmental management

Tocotrienol-rich fraction supplementation modulates antioxidant enzymes activity and reduces DNA damage in APPswe/PS1dE9 Alzheimer’s Disease Mouse Model

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by deterioration of the brain functions that result in impairment of memory, cognition and behavioural functions. Oxidative stress is well known to be one of the causative factors for AD. Thus this disease is potentially modulated by natural antioxidants such as vitamin E. The aim of this study was to evaluate the effect of tocotrienol-rich fraction (TRF) supplementation on antioxidant enzymes and DNA damage using APPswe/PS1dE9 transgenic mouse model of AD. Animals were supplemented with TRF (200 mg/kg) or alpha-tocopherol (αT) (200 mg/kg) for six months starting from nine months old. We found that superoxide dismutase (SOD) activity in AD mouse was decreased by supplementation of TRF and αT as compared with AD control mouse with no significant differences in glutathione peroxidise (GPx) activity in all groups. TRF supplementation significantly increased catalase (CAT) activity. The level of DNA damage of AD mouse shows significant decrease with supplementation of TRF and αT. In conclusion, TRF was able to modulate antioxidant enzymes activity and decreased the level of DNA damage of AD transgenic mouse model

Intestinal microsporidiosis: a new entity in Malaysia?

Objective: Intestinal microsporidia is an emerging human disease caused by microsporidia. A study was conducted to determine the prevalence of microsporidia in patients with gastro-intestinal symptoms and to examine the clinical manifestations associated with intestinal microsporidiosis. Methods: A descriptive cross-sectional study using a well-structured questionnaire; a review of medical records was also undertaken. Positive stool samples were defined as presence of one or more pinkish-violet ovoid structures with a belt-like stripe under high power field (100x) using modified gram-chromotrope stain (MGC). Results: A total of 353 faecal specimens of patients was examined and 100 patients were found to have positive stool samples for microsporidia. The overall prevalence of microsporidia was 28.3%. Acute and chronic diarrhoea were seen in 49.0% and 36.0% patients, respectively. The commonest clinical presentations were diarrhoea (85.0%) with 83.0 % of patients having loose or watery stools, vomiting (75.0%), foul-smelling stools (60.0%), nausea (59.0%) and cramping abdominal pain (39.0%). The least common symptoms were fever (15.0%), mucous in stool (5.0%) and blood in stool (4.0%). Conclusion: This study concludes that the prevalence of microsporidia is still high (28.3%) and the majority of patients (93.0%) are symptomatic; the most common gastro-intestinal symptom is diarrhoea with loose or watery stools. Hence, it is recommended that a stool screening for microsporidia be done in selected patients presented with gastrointestinal symptoms

Signal of harm in morphine use in adults with acute pulmonary oedema: A rapid systematic review

Background. Heart failure affects nearly 65 million people globally, resulting in recurrent hospital admissions and substantial healthcare expenditure. The use of morphine in the management of acute pulmonary oedema remains controversial, with conflicting guidance and significant variation in practice. Synthesised evidence is needed to inform standard treatment guidelines and clinical practice. Objective. To determine whether morphine should be used in the treatment of acute pulmonary oedema (APE) in adults. Methods. A rapid review of systematic reviews of randomised controlled trials or observational studies, and then randomised controlled trials, was conducted searching three electronic databases (PubMed, Embase, Cochrane Library) and one clinical trial registry on 12 February 2022. We used a prespecified protocol following Cochrane rapid review methods and aligned to the National Standard Treatment Guidelines and Essential Medicines List methodology. We first considered relevant high-quality systematic reviews of randomised controlled trials or observational studies, then (if required) randomised controlled trials to inform time-sensitive or urgent evidence requests, clinical practice, policy, or standard treatment guidelines. Results. We identified four systematic reviews of observational studies. The two most relevant, up-to-date, and highest-quality reviews were used to inform evidence for critical outcomes. Morphine may increase in-hospital mortality (odds ratio (OR) 1.78; 95% confidence interval (CI) 1.01 - 3.13; low certainty of evidence; six observational studies, n=151 735 participants), resulting in 15 more per 1 000 hospital deaths, ranging from 0 to 40 more hospital deaths. Morphine may result in a large increase in invasive mechanical ventilation (OR 2.72; 95% CI 1.09 - 6.80; low certainty of evidence; four observational studies, n=167 847 participants), resulting in 45 more per 1 000 ventilations, ranging from 2 more to 136 more. Adverse events and hospital length of stay were not measured across reviews or trials. Conclusion. Based on the most recent, relevant and best-available quality evidence, morphine use in adults with APE may increase in-hospital and all-cause mortality and may result in a large increase in the need for invasive mechanical ventilation compared to not using morphine. Recommending against the use of morphine in pulmonary oedema may improve patient outcomes. Disinvesting in morphine for this indication may result in cost savings, noting the possible accrued benefits of fewer patients requiring invasive ventilation and management of morphine-related side-effects

Latent class analysis: an innovative approach for identification of clinical and laboratory markers of disease severity among COVID-19 patients admitted to the intensive care unit

Objective: The aim of this study was to identify clinical and laboratory phenotype distribution patterns and their usefulness as prognostic markers in COVID-19 patients admitted to the intensive care unit (ICU) at Tygerberg Hospital, Cape Town. Methods and results: A latent class analysis (LCA) model was applied in a prospective, observational cohort study. Data from 343 COVID-19 patients were analysed. Two distinct phenotypes (1 and 2) were identified, comprising 68.46% and 31.54% of patients, respectively. The phenotype 2 patients were characterized by increased coagulopathy markers (D-dimer, median value 1.73 ng/L vs 0.94 ng/L; p < 0.001), end-organ dysfunction (creatinine, median value 79 µmol/L vs 69.5 µmol/L; p < 0.003), under-perfusion markers (lactate, median value 1.60 mmol/L vs 1.20 mmol/L; p < 0.001), abnormal cardiac function markers (median N‐terminal pro‐brain natriuretic peptide (NT-proBNP) 314 pg/ml vs 63.5 pg/ml; p < 0.001 and median high‐sensitivity cardiac troponin (Hs-TropT) 39 ng/L vs 12 ng/L; p < 0.001), and acute inflammatory syndrome (median neutrophil-to-lymphocyte ratio 15.08 vs 8.68; p < 0.001 and median monocyte value 0.68 × 109/L vs 0.45 × 109/L; p < 0.001). Conclusion: The identification of COVID-19 phenotypes and sub-phenotypes in ICU patients could help as a prognostic marker in the day-to-day management of COVID-19 patients admitted to the ICU

Predicting COVID-19 outcomes from clinical and laboratory parameters in an intensive care facility during the second wave of the pandemic in South Africa

Background: The second wave of coronavirus disease 2019 (COVID-19) in South Africa was caused by the Beta variant of severe acute respiratory syndrome coronavirurus-2. This study aimed to explore clinical and biochemical parameters that could predict outcome in patients with COVID-19. Methods: A prospective study was conducted between 5 November 2020 and 30 April 2021 among patients with confirmed COVID-19 admitted to the intensive care unit (ICU) of a tertiary hospital. The Cox proportional hazards model in Stata 16 was used to assess risk factors associated with survival or death. Factors with P<0.05 were considered significant. Results: Patients who died were found to have significantly lower median pH (P<0.001), higher median arterial partial pressure of carbon dioxide (P<0.001), higher D-dimer levels (P=0.001), higher troponin T levels (P=0.001), higher N-terminal-prohormone B-type natriuretic peptide levels (P=0.007) and higher C-reactive protein levels (P=0.010) compared with patients who survived. Increased standard bicarbonate (HCO3std) was associated with lower risk of death (hazard ratio 0.96, 95% confidence interval 0.93–0.99). Conclusions: The mortality of patients with COVID-19 admitted to the ICU was associated with elevated D-dimer and a low HCO3std level. Large studies are warranted to increase the identification of patients at risk of poor prognosis, and to improve the clinical approach

Haematological predictors of poor outcome among COVID-19 patients admitted to an intensive care unit of a tertiary hospital in South Africa

BACKGROUND: Studies from Asia, Europe and the USA indicate that widely available haematological parameters could be used to determine the clinical severity of Coronavirus disease 2019 (COVID-19) and predict management outcome. There is limited data from Africa on their usefulness in patients admitted to Intensive Care Units (ICUs). We performed an evaluation of baseline haematological parameters as prognostic biomarkers in ICU COVID-19 patients. METHODS: Demographic, clinical and laboratory data were collected prospectively on patients with confirmed COVID-19, admitted to the adult ICU in a tertiary hospital in Cape Town, South Africa, between March 2020 and February 2021. Robust Poisson regression methods and receiver operating characteristic (ROC) curves were used to explore the association of haematological parameters with COVID-19 severity and mortality. RESULTS: A total of 490 patients (median age 54.1 years) were included, of whom 237 (48%) were female. The median duration of ICU stay was 6 days and 309/490 (63%) patients died. Raised neutrophil count and neutrophil/lymphocyte ratio (NLR) were associated with worse outcome. Independent risk factors associated with mortality were age (ARR 1.01, 95%CI 1.0–1.02; p = 0.002); female sex (ARR 1.23, 95%CI 1.05–1.42; p = 0.008) and D-dimer levels (ARR 1.01, 95%CI 1.002–1.03; p = 0.016). CONCLUSIONS: Our study showed that raised neutrophil count, NLR and D-dimer at the time of ICU admission were associated with higher mortality. Contrary to what has previously been reported, our study revealed females admitted to the ICU had a higher risk of mortality

Defining a Minimum Set of Standardized Patient-centered Outcome Measures for Macular Degeneration

Purpose To define a minimum set of outcome measures for tracking, comparing, and improving macular degeneration care. Design Recommendations from a working group of international experts in macular degeneration outcomes registry development and patient advocates, facilitated by the International Consortium for Health Outcomes Measurement (ICHOM). Methods Modified Delphi technique, supported by structured teleconferences, followed by online surveys to drive consensus decisions. Potential outcomes were identified through literature review of outcomes collected in existing registries and reported in major clinical trials. Outcomes were refined by the working group and selected based on impact on patients, relationship to good clinical care, and feasibility of measurement in routine clinical practice. Results Standardized measurement of the following outcomes is recommended: visual functioning and quality of life (distance visual acuity, mobility and independence, emotional well-being, reading and accessing information); number of treatments; complications of treatment; and disease control. Proposed data collection sources include administrative data, clinical data during routine clinical visits, and patient-reported sources annually. Recording the following clinical characteristics is recommended to enable risk adjustment: age; sex; ethnicity; smoking status; baseline visual acuity in both eyes; type of macular degeneration; presence of geographic atrophy, subretinal fibrosis, or pigment epithelial detachment; previous macular degeneration treatment; ocular comorbidities. Conclusions The recommended minimum outcomes and pragmatic reporting standards should enable standardized, meaningful assessments and comparisons of macular degeneration treatment outcomes. Adoption could accelerate global improvements in standardized data gathering and reporting of patient-centered outcomes. This can facilitate informed decisions by patients and health care providers, plus allow long-term monitoring of aggregate data, ultimately improving understanding of disease progression and treatment responses