4 research outputs found
Determination of oleuropein in herbal preparation and Olea europaea L. extracts by HPLC
Oleuropein is a secoiridoide monoterpene. It is found primarily in all parts of the
Olea europaea L. tree, mostly in its unripe fruit and leaves. It has a number of beneficial
effects on the human body, being readily absorbed from the gastrointestinal tract
due to its hydrophilic properties.
The paper presents a simple, rapid and precise method for the assay of oleuropein
in herbal formulations and the European Olive leaf extract. Chromatographic separation
was performed using a C-18 column with an acetonitrile/water 20/80% vol. mixture as
the mobile phase. The method has a wide range of linearity between 0.05 and 1.5
mg/mL, very good repeatability of retention time, and accuracies between 95.8 and
103.3%. Its limit of detection is 0.04 mg/mL. The developed method was tested on two
herbal formulations which are commercially available in Poland