Perbedaan Gambaran Histopatologi Granuloma Paru Mencit Setelah Diinfeksi Mycobacterium tuberculosis dan atau Intervensi Silika

The characteristics of lung tuberculosis is granuloma, which is consisted of lymphocytes andmacrophages that show the interaction between immune cells and M.tb. Granuloma is the organizationprocess which is depend on lymphocytes invasion, adhesion molecules and chemokine fasilitation. Silicosiswhich is caused by silica, can influence granuloma in the lung. The features of granuloma is variationdepend on the elicited agent and immune reaction. The main purpose of this study was to prove thehistopathology differences of mice lung granuloma caused by M.tb infection, silica intervention and bothin 3th and 7th weeks. It was 45 mice Balb-c strain, divided into 3 groups; P1 got M.tb infection with H37Rvstrain 105 perml,P2 got silica intervention with 60 micro litre and, P3 got both of M.tb infection and silica intervention. Termination of each group were held on 3 and 7 weeks of intervention, continued byhistopathology examination. In the histopathology feature, we done semi-quantitative prosedure to measurelung damage by using Dormans scores; perivasculitis, peribronchiolitis, alveolitis and granuloma. Oneway anova to analysis the differences of histopathologycal result among these groups (P< 0,05). Resultshowed the significant differences among these group. In the 3th weeks, we found mild lung damage werehappened in all groups with granuloma, without necrosic (P1 and P2). In the 7th weeks we found severe lungdamage in P3 with necrotic and fibrotic granuloma sign, with necrosis in P1, with fibrotic in P2. Weconcluded the worst lung damage happened in 7th weeks in group which are got M.tb infection and silicaintervention, with granuloma characterictic of necrosic and fibrotic

Growth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan (GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIAL PROTEIN EXPRESSION IN MALE RATS)

The use of growth hormone (GH) treatment in aging related condition such as atherosclerosis is stillcontroversial. Previous study showed GH reduce atherosclerotic plaque and prevent endothelial cellsenescence. This study was aimed to understand the mechanism of GH effect to endothelial senescencethrough p53/p21 pathway. A randomized posttest only control group design study was conducted. Twentymale Wistar rats were randomized into five groups; negative control (P0), positive control (P1), and GHtreated group (P2, P3, P4). Negative control group was fed with standard diet, and others were fed withatherogenic diet for 20 weeks. After 10 weeks, subjects were injected subcutaneously (0,1 mL) with aquadest(P0 and P1) and increasing dose of GH (0,02 IU, 0,04 IU, and 0,08 IU) for P2, P3, P4 once a day respectivelyfor 10 weeks. In the end of the study all subjects were examined for p53 and p21 endothelial proteinexpressions. Immunohistochemistry of endothelial p53 showed reduce expression in treated groups (P0:7.28 ± 0.36; P1: 39.51 ± 1.18; P2: 32.70 ± 1.10; P3: 16.98 ± 0.78; and P4: 14.29 ± 0.38). The reduction was also observed in p21 expression (P0: 5.38 ± 0.49; P1: 37.81 ± 0.76; P2: 26.02 ± 1.54; P3: 16.37 ± 1.24; andP4: 4.82 ± 0.61. One way analysis of variance and post hoc test (LSD) analysis showed significant differencesbetween all groups (p<0.05). In conclusion, GH reduces endothelial expression of p53 and p21 and thispathway may contribute to GH effect on atherosclerotic plaque and endothelial senescence

Penurunan Kadar Trigliserida pada Tikus Jantan dengan Terapi Growth Hormone (DECREASE OF TRIGLYCERIDE LEVEL IN MALE RAT BY GROWTH HORMONE TREATMENT)

The use of growth hormone (GH) as cardiovascular disease treatment is still controversial. In thispreliminary study the effect of growth hormone therapy on plasma triglyceride level in dyslipidemia wasexamined. Pre and post control group design study was done using 20 dyslipidemic (total cholesterol &gt;200mg/dL) male rats, age 11–12 month-old. The subjects were divided into four groups, aquadest (P0), GH0.02 IU/day (P1), GH 0.04 IU/day (P2), and GH 0.08 IU/day (P3). All subjects were given high cholesteroldiet for three weeks to achieve dyslipidemic in blood level. Aquadest and GH were injected subcutaneouslyonce daily for two weeks. Triglyceride plasma level was measured on day 22nd and 38th by using colorimetricenzymatic test. The mean of pre test plasma triglyceride level of all groups was 136.30 mg/dL and nosignificant difference was found among the groups (p &gt; 0.05). Growth hormone therapy significantly reducedplasma triglyceride level of P1 by 11.78% (118.82 mg/dL, p &lt; 0.01), P2 by 23.46% (103.41 mg/dL, p &lt; 0.01),and P3 by 35.15% (90.22 mg/dL, p &lt; 0.01). Comparison of  post test data amomg the groups showedsignificant difference (p &lt; 0.01). This study show that growth hormone therapy could reduce plasmatriglyceride level in dyslipidemic rat. However, further research is needed to more understand the effect ofthe therapy on cardiovascular diseases

Perbedaan Gambaran Histopatologi Granuloma Paru Mencit Setelah Diinfeksi Mycobacterium tuberculosis dan atau Intervensi Silika (THE INFLUENCES OF TIME IN THE HISTOPATHOLOGY OF LUNG GRANULOMA IN MICE AFTER INFECTION OF MYCOBACTERIUM TUBERCULOSIS AND SILI

The characteristics of lung tuberculosis is granuloma, which is consisted of lymphocytes andmacrophages that show the interaction between immune cells and M.tb. Granuloma is the organizationprocess which is depend on lymphocytes invasion, adhesion molecules and chemokine fasilitation. Silicosiswhich is caused by silica, can influence granuloma in the lung. The features of granuloma is variationdepend on the elicited agent and immune reaction. The main purpose of this study was to prove thehistopathology differences of  mice lung granuloma caused by M.tb infection,  silica intervention and bothin 3th  and 7th weeks. It was 45 mice Balb-c strain, divided into 3 groups;  P1 got  M.tb infection with H37Rvstrain 105  perml,P2 got silica intervention with 60 micro litre and, P3 got both of M.tb infection and  silica intervention. Termination of each group were held on 3 and 7 weeks of intervention, continued byhistopathology examination. In the histopathology feature, we done semi-quantitative prosedure to measurelung damage by using Dormans scores; perivasculitis, peribronchiolitis, alveolitis and granuloma. Oneway anova to analysis the differences of histopathologycal result among these groups (P&lt; 0,05).  Resultshowed the significant differences  among these group.  In the 3th weeks, we found  mild lung damage werehappened in all groups with granuloma, without necrosic (P1 and P2). In the 7th weeks we found  severe lungdamage in P3 with necrotic and fibrotic granuloma sign, with necrosis in P1, with fibrotic in P2.  Weconcluded the worst lung damage happened in 7th weeks in group which are got M.tb infection and silicaintervention, with granuloma characterictic of necrosic and fibrotic