19 research outputs found
Correction: Human Adipose Tissue-Derived Mesenchymal Stem Cells Target Brain Tumor-Initiating Cells
<p>Correction: Human Adipose Tissue-Derived Mesenchymal Stem Cells Target Brain Tumor-Initiating Cells</p
mRNA expression of cyto-chemokine receptors in human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) after co-culture with brain tumor-initiating cells (BTICs).
<p>Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) analysis of cyto-chemokine receptors after co-culture of (A) medulloblastoma-BTICs, (B) atypical teratoid/rhabdoid tumors (AT/RT)-BTICs and (C) glioblastoma-BTICs. The mRNA level of each cytokine receptor was normalized to the level of GAPDH. All data are representative of three independent experiments, and each value represents the mean ± SD. *Significant difference from control (P < 0.05).</p
Induced cytokine levels in the BTICs after co-cultured with HFF1 or hAT-MSCs (pg/ml)<sup>*</sup>.
<p>BTICs: brain tumor initiating cells, HFF1: human foreskin fibroblast, hAT-MSCs: human adipose-derived mesenchymal stem cells, AT/RT: atypical teratoid rhabdoid tumor, MCP-1: monocyte chemoattractant protein 1, SDF-1: stromal cell-derived factor 1, RANTES: regulated on activation, normal T cell expressed and secreted, IL-6: interleukin-6 ligand, IL-8: interleukin-8, IGF-1: insulin-like growth factor 1ligand, PDGF-bb: platelet-derived growth factor, VEGF: vascular endothelial growth factor, Ang-1: angiopoietin1</p><p>*[induced cytokine level in the BTICs with HFF1] = [total cytokine level in the BTICs with HFF1]-[cytokine level in the HFF1 only]</p><p>[induced cytokine level in the BTICs with hAT-MSCs] = [total cytokine level in the BTICs with hAT-MSCs]-[cytokine level in the hAT-MSCs only]</p><p>Induced cytokine levels in the BTICs after co-cultured with HFF1 or hAT-MSCs (pg/ml)<sup><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129292#t001fn002" target="_blank">*</a></sup>.</p
Migratory ability of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) toward brain tumor-initiating cells (BTICs) <i>in vivo</i>.
<p>Fluorescence images of the brains were taken at the indicated times. (A) In the absence of tumor, the fluorescent signals of hAT-MSCs (blue) are weakened at 2 weeks and are not detectable at 3 weeks. (B) <i>In vivo</i> sequential tracking was performed by injecting both NEO-LIVE 675-labeled BTICs (red) and NEO-LIVE 797-labeled hAT-MSCs (blue). hAT-MSCs gradually migrate toward the tumor site, and strong fluorescent signals are observed at 4 weeks post-injection at the tumor site. (C) Mice were injected the NEO-LIVE 797-labeled hAT-MSCs or HFF1. Representative <i>in vivo</i> fluorescence images show that the HFF1 signals are decreased or fade out. (D) On the contrary, the hAT-MSC signals are widened at all BTIC-derived mouse brain tumor sites at 3 weeks post-injection when compared with HFF1 cells. The color bar indicates radiant efficiency.</p
Knock down of cytokine receptors on hAT-MSCs.
<p>After siRNAs treatment to each cytokine receptors on hAT-MSCs, (A) the protein expressions were confirmed in hAT-MSCs by western blot and (B) the migratory ability toward BTICs is assessed using trans-well assay. (C) The quantified results show inhibition of migration by selective knock down of cytokine receptors. ×50 magnification. All data are representative of three independent experiments, and each value represents the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.</p
Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs-1
<p><b>Copyright information:</b></p><p>Taken from "Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs"</p><p>http://www.biomedcentral.com/1471-2121/8/20</p><p>BMC Cell Biology 2007;8():20-20.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1894962.</p><p></p>3 cell culture protocol. . Senescence-associated β-galactosidase activity (SA-β-gal)-positive cell numbers of the different passages MPF and PF cells. Data shown are means ± SEM (n = 3). The a, b, c, and d indicate significant differences (p < 0.05). . The representative photographs (40× magnification) of different passages MPF and PF cells showing SA-β-gal activity
Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs-7
<p><b>Copyright information:</b></p><p>Taken from "Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs"</p><p>http://www.biomedcentral.com/1471-2121/8/20</p><p>BMC Cell Biology 2007;8():20-20.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1894962.</p><p></p>3 cell culture protocol. . Senescence-associated β-galactosidase activity (SA-β-gal)-positive cell numbers of the different passages MPF and PF cells. Data shown are means ± SEM (n = 3). The a, b, c, and d indicate significant differences (p < 0.05). . The representative photographs (40× magnification) of different passages MPF and PF cells showing SA-β-gal activity
Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs-2
<p><b>Copyright information:</b></p><p>Taken from "Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs"</p><p>http://www.biomedcentral.com/1471-2121/8/20</p><p>BMC Cell Biology 2007;8():20-20.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1894962.</p><p></p> loading control. . Expression of p53 and p21proteins in the primary and immortal MPF and PF cells grown in the absence or presence of doxorubicin (3 uM). α-tubulin was used as a loading control. . Cell viability (%) of the primary and immortal MPF and PF cells grown in the absence or presence of doxorubicin (1, 3, and 5 uM) for 24 hr. The a, b, c, and d indicate significant differences (p < 0.05)
Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs-3
<p><b>Copyright information:</b></p><p>Taken from "Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs"</p><p>http://www.biomedcentral.com/1471-2121/8/20</p><p>BMC Cell Biology 2007;8():20-20.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1894962.</p><p></p>onditions for 3 weeks (Left), and the foci numbers of primary and immortal MPF and PF cells grown in the soft-agar (Right). HeLa cells served as the positive control. The number shown in the graph are means ± SEM (n = 3). Asterisk (*) indicates a significant difference (p < 0.001). . Representative photographs showing metaphase chromosomes of primary and immortal MPF and PF cells (400× magnifications; Left), and average number of chromosomes of primary and immortal MPF and PF cells (Right). The number shown in the graph are means ± SEM (n = 100). Asterisk (*) indicates a significant difference (p < 0.05)
Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs-0
<p><b>Copyright information:</b></p><p>Taken from "Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs"</p><p>http://www.biomedcentral.com/1471-2121/8/20</p><p>BMC Cell Biology 2007;8():20-20.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1894962.</p><p></p>0; P60) (40× magnifications). . Relative growth rates of MPF and PF cells at an early passage (P3) grown in 10% and 0.5% FBS-DMEM as determined by staining cells with crystal violet solution every 2 days for 6 days. Data shown are means ± SEM (n = 3). Asterisk (*) indicates a significant difference between MPF and PF cells grown in the 10% FBS-DMEM (p < 0.05). . Relative growth rates of MPF and PF cells at a late passage (P60) grown in 10% and 0.5% FBS-DMEM. Data shown are means ± SEM (n = 3). Asterisk (*) indicates a significant difference between MPF and PF cells grown in the 10% FBS-DMEM (p < 0.05)