2,524 research outputs found
Comparisons of ELISA and Western blot assays for detection of autophagy flux
We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blotting, and compared their working range, accuracy, and reliability. The ELISAs showed a broader working range than that of the LC3 Western blots (Table 1). Table 2 showed that data value distribution was tighter and the average standard error from the ELISA was much smaller than those of the Western blot, directly relating to the accuracy of the assay. Test-retest reliability analysis showed good reliability for three individual ELISAs (interclass correlation, ≥ 0.7), but poor reliability for three individual Western blots (interclass correlation, ≤ 0.4) (Table 3). Keywords: Autophagy, Mitophagy, ELISA, Western blot, Skeletal muscl
Influence of ripening stages on phytochemical composition and bioavailability of ginseng berry (Panax ginseng C.A. Meyer)
The presence of large amounts of bioactive compounds such as saponins and flavonoids in ginseng (Panax ginseng) berry suggests its potential as a functional resource for the food and medical industries, despite the fact that been considered a useless by-products of P. ginseng. In this study, we examined the variations in the antioxidant and anti-melanogenic potential of ginseng berry during the ripening process. We found that fully ripe berry extracts (Go-S3) contained the highest level of antioxidant and anti-melanogenic activities. Phytochemical screening suggested that alterations in polyphenol contents correlated with the variation in bioactive principles of ginseng berry during the ripening process. Furthermore, results obtained by quantitative real-time PCR, western blot, tyrosinase inhibition assay and molecular docking analysis suggested that Go-S3 probably inhibits tyrosinase activity by interacting with copper-coordinating histidines and second shell residues of tyrosinase, resulting in the reduction of melanin production in α-MSH-stimulated B16F10 cells. Taken together, these finding suggest the potential of ginseng berry as a resource for functional applications in the cosmetic industries and demonstrate that fruit ripening stages have profound effects on the pharmaceutical value of ginseng berry
A Large-Scale 3D Face Mesh Video Dataset via Neural Re-parameterized Optimization
We propose NeuFace, a 3D face mesh pseudo annotation method on videos via
neural re-parameterized optimization. Despite the huge progress in 3D face
reconstruction methods, generating reliable 3D face labels for in-the-wild
dynamic videos remains challenging. Using NeuFace optimization, we annotate the
per-view/-frame accurate and consistent face meshes on large-scale face videos,
called the NeuFace-dataset. We investigate how neural re-parameterization helps
to reconstruct image-aligned facial details on 3D meshes via gradient analysis.
By exploiting the naturalness and diversity of 3D faces in our dataset, we
demonstrate the usefulness of our dataset for 3D face-related tasks: improving
the reconstruction accuracy of an existing 3D face reconstruction model and
learning 3D facial motion prior. Code and datasets will be available at
https://neuface-dataset.github.io.Comment: 9 pages, 7 figures, and 3 tables for the main paper. 8 pages, 6
figures and 3 tables for the appendi
LaughTalk: Expressive 3D Talking Head Generation with Laughter
Laughter is a unique expression, essential to affirmative social interactions
of humans. Although current 3D talking head generation methods produce
convincing verbal articulations, they often fail to capture the vitality and
subtleties of laughter and smiles despite their importance in social context.
In this paper, we introduce a novel task to generate 3D talking heads capable
of both articulate speech and authentic laughter. Our newly curated dataset
comprises 2D laughing videos paired with pseudo-annotated and human-validated
3D FLAME parameters and vertices. Given our proposed dataset, we present a
strong baseline with a two-stage training scheme: the model first learns to
talk and then acquires the ability to express laughter. Extensive experiments
demonstrate that our method performs favorably compared to existing approaches
in both talking head generation and expressing laughter signals. We further
explore potential applications on top of our proposed method for rigging
realistic avatars.Comment: Accepted to WACV202
Two-gap and paramagnetic pair-breaking effects on upper critical field of SmFeAsO and SmFeAsOF single crystals
We investigated the temperature dependence of the upper critical field
[] of fluorine-free SmFeAsO and fluorine-doped
SmFeAsOF single crystals by measuring the resistive transition
in low static magnetic fields and in pulsed fields up to 60 T. Both crystals
show that 's along the c axis [] and in an -planar
direction [] exhibit a linear and a sublinear increase,
respectively, with decreasing temperature below the superconducting transition.
's in both directions deviate from the conventional one-gap
Werthamer-Helfand-Hohenberg theoretical prediction at low temperatures. A
two-gap nature and the paramagnetic pair-breaking effect are shown to be
responsible for the temperature-dependent behavior of and
, respectively.Comment: 21 pages, 8 figure
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