Collective Josephson vortex dynamics in a finite number of intrinsic Josephson junctions

We report the experimental confirmation of the collective transverse plasma modes excited by the Josephson vortex lattice in stacks of intrinsic Josephson junctions in Bi$_{2}$Sr$_{2}$CaCu$_{2}$O$_{8+x}$ single crystals. The excitation was confirmed by analyzing the temperature ($T$) and magnetic field ($H$) dependencies of the multiple sub-branches in the Josephson-vortex-flow region of the current-voltage characteristics of the system. In the near-static Josephson vortex state for a low tunneling bias current, pronounced magnetoresistance oscillations were observed, which represented a triangular-lattice vortex configuration along the c axis. In the dynamic vortex state in a sufficiently high magnetic field and for a high bias current, splitting of a single Josephson vortex-flow branch into multiple sub-branches was observed. Detailed examination of the sub-branches for varying $H$ field reveals that sub-branches represent the different modes of the Josephson-vortex lattice along the c axis, with varied configuration from a triangular to a rectangular lattices. These multiple sub-branches merge to a single curve at a characteristic temperature, above which no dynamical structural transitions of the Josephson vortex lattice is expected

Heating-compensated constant-temperature tunneling measurements on stacks of Bi2_2Sr2_2CaCu2_2O8+x_{8+x} intrinsic junctions

In highly anisotropic layered cuprates such as Bi$_2$Sr$_2$CaCu$_2$O$_{8+x}$ tunneling measurements on a stack of intrinsic junctions in a high-bias range are often susceptible to self-heating. In this study we monitored the temperature variation of a stack ("sample stack") of intrinsic junctions by measuring the resistance change of a nearby stack ("thermometer stack") of intrinsic junctions, which was strongly thermal-coupled to the sample stack through a common Au electrode. We then adopted a proportional-integral-derivative scheme incorporated with a substrate-holder heater to compensate the temperature variation. This in-situ temperature monitoring and controlling technique allows one to get rid of spurious tunneling effects arising from the self-heating in a high bias range.Comment: 3 pages, 3 figure

Optimal design of quadratic electromagnetic exciter

The vibration acceleration of collecting plates, which is the core indicator of rapping performance in an electrostatic precipitator’s vibration rapping process, is determined by magnetic force of a quadratic electromagnetic exciter. The larger exciter provides the larger magnetic force, but the installation space for the exciter is limited. Accordingly, this paper presents the optimal design of quadratic electromagnetic exciter to maximize the magnetic force with constraint that the size of exciter is constant. A design optimization problem was formulated in order to find the quadratic electromagnetic exciter shape parameters that maximized the magnetic force. The magnetic force of the quadratic electromagnetic exciter was evaluated using the commercial electromagnetic analysis software “MAXWELL”. For efficient design, we employed metamodel-based design optimization using design of experiments (DOE), metamodels, and an optimization algorithm equipped in PIAnO (Process Integration, Automation and Optimization), a commercial PIDO (Process Integration and Design Optimization) tool. Using the proposed design approach, the optimal magnetic force was increased by 1.68 % compared to the initial one. This result demonstrates the effectiveness of the established analysis and design procedure for the quadratic electromagnetic exciter

Strategies for the Endoscopic Management and Post-Resection Treatment of Superficial Esophageal Squamous Cell Carcinoma

Endoscopic resection (ER) is preferred for treating superficial esophageal squamous cell carcinoma (SESCC) that has a low risk of lymph node metastasis; the preference is due to the technique’s minimal invasiveness and having outcomes comparable to those of surgical resection, in suitable cases. Determining the appropriateness of ER requires a multidisciplinary review involving a pathological examination of the tumor’s depth, size, and lymphovascular invasion following resection of a specimen. According to the Korean guidelines, no additional treatment is necessary after a complete en bloc resection of SESCC that lacks submucosal invasion and lymphovascular invasion. In cases of non-curative resection for tumors that exhibit submucosal invasion, lymphovascular invasion, or positive margins, additional treatment (e.g., esophagectomy or chemoradiotherapy) is recommended. Due to the uncertainty regarding which treatment is more effective, tailored therapy that is based on patient factors and tumor characteristics is needed to maximize patient long-term survival and improve quality of life

DNA Damage Causes p27^(Kip1) Accumulation Through COP1 Signaling

p27 is a critical CDK inhibitor involved in cell cycle regulation, but its response to DNA damage remains unclear. Constitutive photomorphogenesis 1 (COP1), a p53- targeting E3 ubiquitin ligase, is downregulated by DNA damage, but the biological consequences of this phenomenon are poorly understood. Here, we report that p27 levels were elevated after DNA damage, with concurrent reduction of COP1 levels. Mechanistic studies showed that COP1 directly interacted with p27 through a VP motif on p27 and functions as an E3 ligase of p27 to accelerate the ubiquitin-mediated degradation of p27. Also, COP1 overexpression lead to cytoplasmic distribution of p27, thereby accelerating p27 degradation. COP1 overexpression resulted in elevation of Aurora A kinase. COP1 and Aurora A levels were positively correlated in patient samples and associated with poor overall survival. We found that COP1 expression promoted cell proliferation, cell transformation, and tumor progression, manifesting its role in cancer promotion whereas p27 negatively regulated COP1 function and prevented tumor growth in a mouse xenograft model of human cancer. Together, these findings define a mechanism for posttranslational regulation of p27 after DNA damage that can explain the correlation between COP1 overexpression and p27 downregulation during tumorigenesis

COP 9 SIGNALOSOME SUBUNIT 6 STABILIZES COP1, A NOVEL E3 UBIQUITIN LIGASE FOR 14-3-3σ

14-3-3σ, a gene upregulated by p53 in response to DNA damage, exists as part of a positive-feedback loop which activates p53 and is a human cancer epithelial marker downregulated in various cancer types. 14-3-3σ levels are critical for maintaining p53 activity in response to DNA damage and regulating signal mediator such as Akt. Here, we identify Mammalian Constitutive Photomorphogenic 1 (COP1) as a novel E3 ubiquitin ligase for targeting 14-3-3σ through proteasome degradation. We show for the first time that COP9 signalosome subunit 6 (CSN6) associates with COP1 and is involved in 14-3-3σ ubiquitin-mediated degradation. Mechanistic studies show that CSN6 expression leads to stabilization of COP1 through reducing COP1 self-ubiquitination and decelerating COP1’s turnover rate. We also show that CSN6-mediated 14-3-3σ ubiquitination is compromised when COP1 is knocked down. Thus, CSN6 mediates 14-3-3σ ubiquitination through enhancing COP1 stability. Subsequently, we show that CSN6 causes 14-3-3σ downregulation, thereby activating Akt and promoting cell survival by suppressing FOXO, an Akt target, transcriptional activity. Also, CSN6 overexpression leads to increased cell growth, transformation and promotes tumorigenicity. Significantly, 14-3-3σ expression can correct the abnormalities mediated by CSN6 expression. These data suggest that the CSN6-COP1 axis is involved in 14-3-3σ degradation, and that deregulation of this axis will promote cell growth and tumorigenicity