THE EFFECT OF RESISTANCE AND ENDURANCE EXERCISE TRAINING ON MUSCLE PROTEOME EXPRESSION IN HUMAN SKELETAL MUSCLE

To investigate the effect of resistance and endurance training on muscle proteome expression, samples of vastus lateralis from 10 physically active young men were analysed by 2-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differential patterns of protein expression were determined after 4 weeks of endurance or resistance exercise training. Following endurance exercise training, carbonic anhydrase III immunoglobulin heavy chain, myosin heavy chain 1, titin, chromosome 12, and fructose-1,6-bisphosphatase 2 were up-regulated while pyruvate kinase 3 isoform, ubiquitin carboxyl-terminal hydrolase, and phosphoglucomutase were down-regulated. After the 4 weeks of resistance exercise training, five proteins, apolipoprotein A-IV precursor, microtubule-actin cross linking factor 1, myosin light chain, growth hormone inducible transmembrane protein, and an unknown protein were up-regulated and pyruvate kinase 3 isoform, human albumin, and enolase 3 were down-regulated. We conclude that endurance and resistance exercise training differently alter the expression of individual muscle proteins, and that the response of muscle protein expression may be associated with specific myofibre adaptations to exercise training. Proteomic studies represent one of the developing techniques of metabolism which may substantially contribute to new insights into muscle and exercise physiology

Effect of Software Feature Training on Beliefs, Use, and Performance: Using the Benford’s Law Feature of Generalized Audit Software

This experimental research investigated the effect of software training at the feature level, an area infrequently studied in technology training research. We have adopted the beliefs, use, and performance constructs from adoption and training research, and tested them with the Benford’s Law feature, an advanced software feature of Generalized Audit Software (GAS). Through the analysis of 56 audit professionals, we found that software feature training increased the belief of using software features and the use of those software features. However, software feature training did not have an immediate effect on performance. Rather, auditors spent more time practicing the Benford’s Law feature beyond the supplied training material and applying the Benford’s Law feature to other audit tasks

NANOSCALE PATTERNING WITH DNA NANOSTRUCTURES

Predictable and programmable Watson-Crick base pairing between DNA strands makes DNA not only a hereditary material, but also an ideal building block for designing nanometer-scale structures. Since Seeman opened the door to the era of DNA nanotechnology in 1982 by introducing the idea of utilizing DNA to build a mechanically robust four-arm junction structure to the scientific community, there has been an explosive growth in both structural DNA nanotechnology and DNA-based nanofabrication in the past few decades. This dissertation focuses on the nanoscale patterning of soft and hard materials with DNA nanostructures. Chapter two specifically presents an advanced nanoimprint lithography method to construct polymer stamps with negative tone patterns using one- to three-dimensional DNA nanostructures to transfer patterns with high fidelity. The resulting polymer stamps further serve as molds to transfer the patterns to positive imprints on other polymer films. Chapter three presents a method to increase the stability of DNA nanostructure templates through conformal coating with a nanometer-thin protective inorganic oxide layer created using atomic layer deposition. Chapter four presents a new method of direct high contrast pattern transfer from DNA nanostructures to a silicon substrate by reactive ion etching with the help of calcium chloride. This study is the first report on high contrast pattern transfer from unmodified DNA nanostructures to silicon. We hope this dissertation could encourage future work to reveal the true power of DNA-based nanofabrication

An efficient and robust HPLC method to determine the sialylation levels of human epithelial cells

Sialyltransferase, an enzyme responsible for attaching sialic acid to the cell surface, is reported to play a key role in cancer, making sialyltransferase a potential therapeutic target in drug development. Several methods have been developed to quantify sialic acids in biological samples however limitations exists and quantification in complex cell matrices lack investigation. Hence, this paper outlines a simple method to detect and quantify sialic acids in cancer cells for evaluating sialyltransferase activity of potential therapeutic compounds. An efficient method was developed using a reverse-phase ion-pairing HPLC-UV using triisopropanolamine as the ion-pairing agent with a C18 column. Neu5Ac was successfully eluted with the retention time 6.344 min with a flow rate of 0.4 mL/min. The proposed method was validated appropriately according to the AOAC guidelines (2013). This work demonstrates that the proposed method is not only relatively simple but also cost and time effective compared to pre-existing methods to successfully determine both free and protein-bound Neu5Ac in a complex cancer cell matrix. Furthermore, by applying the proposed method, a statistically significant decrease was observed for both HeLa and HuCCT1 cell lines with the application of deoxycholic acid–a known sialyltransferase inhibitor. Hence, the proposed method seems promisingly applicable to evaluate the effectiveness of potential sialyltransferase inhibitors

Combined DNA Methylation and Gastric Microbiome Marker Predicts Helicobacter pylori-Negative Gastric Cancer.

BACKGROUND/AIMS: While DNA methylation and gastric microbiome are each associated with gastric cancer (GC), their combined role in predicting GC remains unclear. This study investigated the potential of a combined DNA methylation and gastric microbiome signature to predict Helicobacter pylori-negative GC. METHODS: In this case-control study, we conducted quantitative methylation-specific polymerase chain reaction to measure the methylation levels of DKK3, SFRP1, EMX1, NKX6-1, MIR124-3, and TWIST1 in the gastric mucosa from 75 H. pylori-negative patients, including chronic gastritis (CG), intestinal metaplasia (IM), and GC. A combined analysis of DNA methylation and gastric microbiome, using 16S rRNA gene sequencing, was performed in 30 of 75 patients. RESULTS: The methylation levels of DKK3, SFRP1, EMX1, MIR124-3, and TWIST1 were significantly higher in patients with GC than in controls (all q<0.05). MIR124-3 and TWIST1 methylation levels were higher in patients with IM than those with CG and also in those with GC than in those with IM (all q<0.05). A higher methylation level of TWIST1 was an independent predictor for H. pylori-negative GC after adjusting for age, sex, and atrophy (odds ratio [OR], 15.15; 95% confidence interval [CI], 1.58 to 145.46; p=0.018). The combination of TWIST1 methylation and GC microbiome index (a microbiome marker) was significantly associated with H. pylori-negative GC after adjusting for age, sex, and atrophy (OR, 50.00; 95% CI, 1.69 to 1,476; p=0.024). CONCLUSIONS: The combination of TWIST1 methylation and GC microbiome index may offer potential as a biomarker for predicting H. pylori-negative GC

Philosophical Analysis of the Name of the Discipline ‘Physical Education’ and Proposal of Alternative Terminology

OBJECTIVES The primary objective of this study is to clarify the concepts of physical education and sport from a philosophical perspective and propose the potential of kinesiology as a promising alternative. METHODS The method of philosophical argumentation was employed to carefully examine the logical consistency of physical education, sport, and kinesiology. The conceptual tools of connotation and denotation were used to analyze these definitions, and their compatibility was thoroughly assessed. RESULTS Through deductive analysis, it was discovered that both physical education and sport face inherent challenges of limited externalization and excessive externalization. These challenges hinder the accurate representation of reality or weaken the distinct identity of the disciplines. Conversely, it was argued that kinesiology possesses the conceptual capacity to encompass both physical education and sport as a discipline. CONCLUSIONS Considering the apparent conceptual constraints of physical education, it becomes reasonable to reassess the nomenclature of our field. This paper has demonstrated the philosophical significance of kinesiology and its potential as a viable alternative. However, since philosophical arguments may not always perfectly align with practical reality, further exploration and evaluation from diverse perspectives are necessary to determine the suitability of adopting the term ‘kinesiology’ as an alternative

Controllable modification of transport properties of single-walled carbon nanotube field effect transistors with in situ Al decoration

We use an in situ Al decoration technique to control the transport characteristics of single-walled carbon nanotube field effect transistors (SWNT-FETs). Al nanoparticle decoration in a high vacuum caused the devices to change from p -type to n -type FETs, and subsequent exposure to the ambient atmosphere induced a gradual recovery of p -type character. In comparison with the bare SWNT-FETs under high vacuum, the channel-open devices with decorated Al particles exhibited reduced current under ambient conditions. However, selective Al decoration only at the contact resulted in an improved p -type current in ambient air.open182

Electrochemical Investigation of High-Performance Dye-Sensitized Solar Cells Based on Molybdenum for Preparation of Counter Electrode

In order to improve the photocurrent conversion efficiency of dye-sensitized solar cells (DSSCs), we studied an alternative conductor for the counter electrode and focused on molybdenum (Mo) instead of conventional fluorine-doped tin oxide (FTO). Because Mo has a similar work function to FTO for band alignment, better formability of platinum (Pt), and a low electric resistance, using a counter electrode made of Mo instead of FTO lead to the enhancement of the catalytic reaction of the redox couple, reduce the interior resistance of the DSSCs, and prevent energy-barrier formation. Using electrical measurements under a 1-sun condition (100 mW/cm(2), AM 1.5), we determined that the fill factor (FF) and photocurrent conversion efficiency (eta) of DSSCs with a Mo electrode were respectively improved by 7.75% and 5.59% with respect to those of DSSCs with an FTO electrode. Moreover, we have investigated the origin of the improved performance through surface morphology analyses such as scanning electron microscopy and electrochemical analyses including cyclic voltammetry and impedance spectroscopy

What are the phases of change in exercise behaviors (EB), and factors affecting exercise behaviors (EB) of male workers in a workplace setting?

The purpose of this study was to evaluate the phases of change of exercise among male workers and to analyze the factors affecting their EB using Information-Motivation-Behavioral skill-Revealed Related Variables (IMBR) model. The study included 163 male workers from a major Hyundai Transys company, Seosan city. Data were analyzed using Pearson’s correlation coefficients, and hierarchical regression etc. Regarding the phases of change in exercise, 135 individuals (82.8%) were classified into phase 3 (preparation phase), phase 4 (action phase), and phase 5 (maintenance phase). In the first step, factors such as health status (β = 0.26 , p < 0.001), smoking (β = 0.16, p = 0.005), number of exercises per week (β = 0.35, p < 0.001), times of each exercise (β = 0.17, p = 0.005), and phases of change in exercise (β = 0.17, p = 0.014) were identified as significant factors affecting EB. In the second step, health status (β = 0.19, p = 0.001), smoking (β = −0.13, p = 0.019), number of exercises per week (β = 0.31, p < 0.001), phases of change in exercise (β = 0.13, p = 0.034), and sport commitment (β = 0.16, p = 0.019) were identified as significant factors. In the third step, health status (β = 0.27, p < 0.001), number of exercises per week (β = 0.14, p = 0.005), and exercise self-efficacy (β = 0.39, p < 0.001) were identified as significant factors, explaining 68.3% of the variance in EB. To promote EB, it’s important to assess the phases of change in exercise and consider factors such as health status, smoking, the number of exercises per week and the duration of each exercise. Interventions that enhance sport commitment and exercise self-efficacy should be considered. It’s recommended to apply IMBR model in exercise studies for workers