30 research outputs found

    The Development and Validation of a Comprehensive List of Primary Sources in College Reading Instruction

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    It may be argued that a field only comes of age when its professional membership is able to gain insight into the present and begin to predict the future through the organized studying and the collective valuing of the field\u27s past. As we enter the 1990\u27s, it is time for the field of college reading and learning assistance to achieve a broader perspective that more fully incorporates the field\u27s rich and varied past. To achieve this end, collectively we should endeavor to understand our professional roots through chronicling, interpreting, and evaluating the fundamental ideas, the pedagogical achievements, and the research contributions of our colleagues, both past and present

    Hydrogel-coated microelectrode array for neural interface

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    Recently, hydrogel has been extensively used in various biomedical applications including soft contact lenses, wound healing materials and as a means for controlled drug delivery and release. In this study, we propose to use hydrogel as a novel neural interface. Planar microelectrode arrays were coated with hydrogel in order to improve the quality of the interface and to aid in drug delivery to cells on microelectrode arrays (MEAs).This work was supported by the International Collaboration Program, NBS-ERC (Nano Bioelectronics and Systems Engineering Research Center)/ KOSEF (Korea Science and Engineering Foundation) and also supported in part by the Nanobiotechnology Center (NBTC), an STC Program of the National Science Foundation under Agreement No. ECS-9876771

    Optical Monitoring of Neural Network Connectivity Using FM1-43-Evoked Activity from Focal Stimulation of Microelectrode Arrays

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    This work was supported by the International Collaboration Program NBS-ERC/KOSEF (S.J.K) and by the National Institute of Biomedical Imaging and Bioengineering under Agreement Number R21-EB007782 (M.R.H)

    Synaptic Connectivity of a Low Density Patterned Neuronal Network Produced on the Poly-L-Lysine Stamped Microelectrode Array

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    Rectangular networks of rat hippocampal neurons have been produced on microelectrode arrays (MEAs). The crossing points of networks were located at the recording electrode sites by aligned microcontact printing (muCP) technique. Polydimethysiloxane (PDMS) stamp was fabricated to print fine poly-L-lysine (PLL) patterns of 2 mum-width lines for neurites and 20 mum-diameter circles for cell bodies. Different densities of neurons were applied on the PLL-stamped MEAs to find how a low density of neurons still has the functional connectivity. From the neural network applied with a density of 200 cells/mm2 , we could observe signal propagation among spontaneous activities. Electrical responses were also evoked by 200 muA current pulse stimulation with 50 mus pulse width. Immunocytostaining was employed to identify dendrites, synapses, and nuclei in the patterned neurons.This work was supported by the International Collaboration Program, NBS-ERC(Nano Bioelectronics and Systems Research Center)/KOSEF(Korea Science and Engineering Foundation) and NIH, NS-044287, NSF, ECS-9876771

    Development of Hybrid Neural Prosthetic Devices

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    This work was supported by the National Institute of Biomedical Imaging and Bioengineering under Agreement Number R21-EB007782 (M.R.H)

    Modulation of Cultured Neural Networks Using Neurotrophin Release

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    Polyacrylamide and poly(ethylene glycol) diacrylate hydrogels were synthesized and characterized for use as drug release and substrates for neuron cell culture. Protein release kinetics was determined by incorporating bovine serum albumin (BSA) into hydrogels during polymerization. To determine if hydrogel incorporation and release affect bioactivity, alkaline phosphatase was incorporated into hydrogels and a released enzyme activity determined using the fluorescence-based ELF-97 assay. Hydrogels were then used to deliver a brain-derived neurotrophic factor (BDNF) from hydrogels polymerized over planar microelectrode arrays (MEAs). Primary hippocampal neurons were cultured on both control and neurotrophin-containing hydrogel-coated MEAs. The effect of released BDNF on neurite length and process arborization was investigated using automated image analysis. An increased spontaneous activity as a response to the released BDNF was recorded from the neurons cultured on the top of hydrogel layers. These results demonstrate that proteins of biological interest can be incorporated into hydrogels to modulate development and function of cultured neural networks. These results also set the stage for development of hydrogel-coated neural prosthetic devices for local delivery of various biologically active molecules.This work was supported by the International Collaboration Program, Nano Bioelectronics and Systems Engineering Research Center/Korea Science and Engineering Foundation (R11-2000-075-00002-0), by the Nanobiotechnology Center (NBTC), an STC Program of the National Science Foundation under agreement no. ECS-9876771, the National Institutes of Health under agreement no. R01-NS044287 (WS) and by the National Institute of Biomedical Imaging and Bioengineering under agreement no. R21EB007782 (MRH). The computational image analysiswas supported by the Center for Subsurface Sensing and Imaging Systems (NSF EEC- 9986821). The authors acknowledge use of the Wadsworth Center Advanced Light Microscopy & Image Analysis Core Facility. They would also like to thank Shirley Madewell and Adriana Verschoor for critical review of the manuscript
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