Enhancing Finite Mathematics with Global Awareness

This paper describes the project of modifying the course, Finite Mathematics, to meet a Global Awareness course requirement. The components of the revised course are described, including topics, in-class activities, and student evaluation methods, along with a summary of the results of a general education assessment of the course

Impact of a New Introductory Mathematical Modeling Course on Student Confidence in Mathematical Ability and Skills

Interdisciplinary mathematics and science courses are increasing in popularity. Faculty teaching these courses are given the opportunity to show how mathematics plays an important role in science and how it can be used to improve our understanding of mathematics and science. This paper discusses a new course in mathematical modeling that focuses on environmental issues. Course content and format are presented, as well as the results of a study on the changes in students’ perceptions of their mathematical abilities as a result of taking this new course

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex

Peptidoglycan (PG) is an essential structural component of the bacterial cell wall that is synthetized during cell division and elongation. PG forms an extracellular polymer crucial for cellular viability, the synthesis of which is the target of many antibiotics. PG assembly requires a glycosyltransferase (GT) to generate a glycan polymer using a Lipid II substrate, which is then crosslinked to the existing PG via a transpeptidase (TP) reaction. A Shape, Elongation, Division and Sporulation (SEDS) GT enzyme and a Class B Penicillin Binding Protein (PBP) form the core of the multi-protein complex required for PG assembly. Here we used single particle cryo-electron microscopy to determine the structure of a cell elongation-specific E. coli RodA-PBP2 complex. We combine this information with biochemical, genetic, spectroscopic, and computational analyses to identify the Lipid II binding sites and propose a mechanism for Lipid II polymerization. Our data suggest a hypothesis for the movement of the glycan strand from the Lipid II polymerization site of RodA towards the TP site of PBP2, functionally linking these two central enzymatic activities required for cell wall peptidoglycan biosynthesis