Crikvenica\u27s cemetries

Autor u radu donosi povijesni pregled posljednjih počivališta, od najstarijih prapovijesnih nalaza na predjelu Stolnič, nalaza iz razdoblja antike u sklopu rimskoga Ad Turresa, srednjovjekovnog groblja Stranče-Gorica u bliskom zaleđu crikvenice, zatim tri groblja vezana uz crkve: sv. Šimuna i Jude Tadeja u naselju Kotor, sv. Antona na Gorici i pavlinske Crkve BDM na ušću Dubračine, jednog kratkotrajno korištenog groblja na lokaciji današnjeg Gradskog kupališta, do sadašnjega i budućega crikveničkog groblja koje postoji tek u planskim dokumentima. Povijest crikveničkih groblja je zapravo povijest samoga mjesta Crikvenice i njegove urbanizacije. S porastom broja stanovnika, koji se u posljednjih 150 godina povećao za gotovo tri i pol puta, rastu potrebe za novim ukopištima. Stoga se koncem 18. i početkom 19. stoljeća otvaraju tri groblja na novim lokacijama, ali se iz urbanističkih razloga dva groblja zatvaraju, a ukopi sele na sadašnje crikveničko groblje. Prostorni resursi postojećeg groblja su do krajnosti potrošeni, pa je izrađena planska dokumentacija za novo groblje u blizini crikveničkog naselja Zoričići, ispod vrha Drenin.In the paper the author gives an overview of the final resting places, from the oldest prehistoric finds at the area of Stolnič, finds from Antiquity within the Roman Ad Turres, the Stranče-Gorica mediaeval cemetery in the nearby hinterland of Crikvenica, then the three cemeteries connected to the churches: Saints Simon and Jude Thaddeus in the settlement of Kotor, St Anthony on Gorica and the Pauline Church of the Blessed Virgin Mary at the mouth of the Dubračina, a single use cemetery at the site of the town’s current bathing area, to the present-day and future Crikvenica cemetery which exists only in planning documents. The history of Crikvenica’s cemeteries is in fact the history of the town of Crikvenica itself and its urbanisation. With the growth of the population, which over the last 150 years has increased almost three and a half times, the need for new burial grounds has also grown. Therefore, at the end of the 18th century and the beginning of the 19th three cemeteries opened in new locations, however for urbanisation reasons two cemeteries were closed, and the burials moved to the current Crikvenica cemetery. The spatial resources of the existing cemetery have been used to the extremes, so planning documentation has been made for a new cemetery near to the Crikvenica settlement of Zoričići, under the hilltop of Drenin

Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting

Auxin is essential for regulating plant growth and development as well as the response of plants to abiotic stresses. AUX/LAX proteins are auxin influx transporters belonging to the amino acid permease family of proton-driven transporters, and are involved in the transport of indole-3-acetic acid (IAA). However, how AUX/LAX genes respond to abiotic stresses in Chinese hickory is less studied. For the first time identification, structural characteristics as well as gene expression analysis of the AUX/LAX gene family in Chinese hickory were conducted by using techniques of gene cloning and real-time fluorescent quantitative PCR. Eight CcAUX/LAXs were identified in Chinese hickory, all of which had the conserved structural characteristics of AUX/LAXs. CcAUX/LAXs were most closely related to their homologous proteins in Populus trichocarpa , which was in consistence with their common taxonomic character of woody trees. CcAUX/LAXs exhibited different expression profiles in different tissues, indicating their varying roles during growth and development. A number of light-, hormone-, and abiotic stress responsive cis-acting regulatory elements were detected on the promoters of CcAUX/LAX genes. CcAUX/LAX genes responded differently to drought and salt stress treatments to varying degrees. Furthermore, CcAUX/LAX genes exhibited complex expression changes during Chinese hickory grafting. These findings not only provide a valuable resource for further functional validation of CcAUX/LAXs, but also contribute to a better understanding of their potential regulatory functions during grafting and abiotic stress treatments in Chinese hickory

Reliability evaluation of electromechanical braking system of mine hoist based on fault tree analysis and Bayesian network

Electromechanical braking system is the key way to improve the braking response ability of mine hoist. At present, the reliability research of electromechanical braking system is less. In order to further analyze and improve the reliability of electro-mechanical braking system, this paper adopts the reliability analysis method of electro-mechanical braking system based on fault tree and Bayesian network. Firstly, the fault tree of the electro-mechanical braking system is established, and then the fault tree is transformed into a Bayesian network, and the posterior probability, probability importance and key importance of each root node are inversely deduced. The diagnosis results show that the ball screw is the weakest link of the electro-mechanical braking system. Then the static simulation and fatigue life simulation of the ball screw are carried out for optimization, and the optimal model of the ball screw is determined. Finally, the electro-mechanical brake installed with the optimized ball screw is tested and analyzed. After the reliable performance test of the electro-mechanical brake, it is finally determined that the braking effect of the optimized electro-mechanical brake is stable

Electro-mechanical braking system spindle unbalance axis trajectory purification and feedback method

The brake disc spindle of the electromechanical braking system will be accompanied by vibration unbalanced fault during the rotation operation, which will affect the braking performance of the brake system. In view of this phenomenon, based on the ensemble empirical mode decomposition algorithm and related energy operation theory, an offline purification program for the unbalanced axial trajectory of the spindle of electromechanical braking system are designed. Meanwhile, an online braking control feedback procedure for the axial trajectory are designed based on Cspace controller. Based on the designed program and braking theory, an experimental bench of the electro-mechanical braking system was set up, and experiments were conducted on the purification of the axial trajectory and the braking of the fault feedback respectively. The results show that the EEMD algorithm and the related energy operation theory can purify the unbalanced axial trajectory of the brake discs of the braking system and draw an ideal axial trajectory fault map; meanwhile, the experimental data of the fault feedback braking shows that the time required from the unbalanced fault monitoring to the completion of the braking process of the braking system is 1.278 s, which can effectively achieve the purpose of emergency braking in case of sudden failure. Through research, a new idea is provided for the development of electro-mechanical braking system fault detection and feedback

Ultrasensitive Ambient Mass Spectrometry Immunoassays: Multiplexed Detection of Proteins in Serum and on Cell Surfaces

The sensitive and accurate determination of multiple protein biomarkers for clinical diagnosis is in high demand. Here, an ambient mass spectrometry immunoassay platform that possesses advantages of high sensitivity, multiplexed quantitation, low sample consumption and convenient operation was established. A series of extensible rhodamine-based mass tags that ensured quantification of multiple proteins with ultrahigh sensitivity through two-stage signal amplification were developed. Thrombin was detected at zeptomole sensitivity in 2 mu L serum/plasma, as was free cancer antigen 125 (CA125). Three protein biomarkers (CA125, carcinoembryonic antigen, and epithelial cell adhesion molecule) were simultaneously detected in situ on ca. 20 cells. This platform is promising for multiple protein detection in a single drop of sample or at the single-cell scale for clinical diagnosis and therapy

Design and experimental study of electrical and mechanical brake for mine hoist

To meet the requirement of the braking response of the coal mine hoist, a new electromechanical braking technology for mine hoists is proposed, the principle of electromechanical braking of mine hoists is demonstrated, and the detailed parameters and braking performance of electromechanical brakes are given. Index, an electromechanical brake test platform with large load and high response is developed. Experiments show that the maximum positive pressure of the designed electromechanical brake reaches 33 KN, which meets the requirement of positive pressure of mine hoist. The braking error is less than 10 %, and the braking gap elimination time is less than 0.1 s. There is a linear relationship between motor current input and brake positive pressure output, with a slope of 4.17 and an intercept of 0.62. The screw displacement output and the brake pressure output have a cubic relationship, and the zero error is small. Through research, a new idea is provided for the development of electromechanical brakes for coal mine hoist

Novel nanomaterials used for sample preparation for protein analysis

Sample preparation is of vital importance for proteomic analysis because of the high complexity of biological samples. The rapid development of novel nanomaterials with various compositions, morphologies, and proper surface modifications provides a category of powerful tools for the sample preparation for protein analysis. In this paper, we have summarized recent progresses for the applications of novel nanomaterials in sample preparation for the analysis of proteomes, especially for phosphoproteomes, glycoproteomes, and peptidoms. Several kinds of novel nanomaterials were also discussed for their use in other kinds of proteomics analysis

Post-synthetic modification of an amino-functionalized metal-organic framework for highly efficient enrichment of N-linked glycopeptides

A maltose-functionalized metal-organic framework (MOF), MIL-101(Cr)-maltose, was developed via a simple two step post-synthetic modification of MIL-101(Cr)-NH2. With the use of this nanomaterial, 33 glycopeptides were detected from the digest of human immunoglobulin G, demonstrating its high efficiency in glycoproteomic analysis. More importantly, the generic functionalization route from amino-derived MOFs opens a new perspective in material design in sample preparation

A dielectric barrier discharge ionization based interface for online coupling surface plasmon resonance with mass spectrometry

The online combination of surface plasmon resonance (SPR) with mass spectrometry (MS) could be beneficial for accurately acquiring molecular interaction data simultaneously with their structural information at high throughputs. In this work, a novel SPR-MS interface was developed using a dielectric barrier discharge ionization (DBDI) source. The DBDI source was placed in front of the MS inlet, generating an ionization plasma jet. A spray tip was set between the DBDI source outlet and the MS inlet, nebulizing the SPR sample solution. Using this interface, samples could first be studied by SPR, then sprayed and ionized, finally analyzed by MS. By analyzing model samples containing small-molecule drugs dissolved in salt containing solutions, the practicability of this SPR-DBDI-MS interface was proved, observing the consistent change of SPR and MS signals. Compared with our previously developed direct analysis in real time (DART) based SPR-MS interface, this new interface exhibited a higher and better tolerance to non-volatile salts, and different ionization capabilities for various samples. These results indicated that the interface could find further utilization in SPR-MS studies especially when physiological conditions were needed