Estrogen and inflammation modulate estrogen receptor alpha expression in specific tissues of the temporomandibular joint

BACKGROUND: Estrogen is known to play role in temporomandibular joint (TMJ) disorders and estrogen effects can be mediated by estrogen receptor (ER) alpha present in the TMJ. Cells expressing the estrogen receptor ERalpha are present in the temporomandibular joint (TMJ) but changes in expression due to estrogen and inflammation have not been characterized. In this study, ERalpha protein content and the number of cells expressing ERalpha was measured in 17 beta-estradiol-treated rats after inflammation was induced in the TMJ. METHODS: Sixteen ovariectomized female rats were divided into two groups such that one group received 17 beta estradiol (E2) and the other was given vehicle (VEH). Groups were then subdivided further, one received injections of saline and the other received Complete Freund's adjuvant (CFA) within the superior joint space of the TMJ. Thus the four groups include no E2/saline, E2/saline, no E2/CFA and E2/CFA. After treatment, the rats were sacrificed, and the TMJ anterior, disc, retrodiscal and synovial tissues were analyzed by western blot and immunocytochemistry. Positive stained cells were counted using a Nikon epifluorescent microscope. RESULTS: The western blot showed that ERalpha protein significantly decreased with inflammation. The number of ERalpha-positive cells in the TMJ was not affected by inflammation or 17 beta-estradiol with exception of the retrodiscal tissue. In the retrodiscal tissue 17 beta-estradiol significantly decreased the number of ERalpha-positive cells but only in a non-inflamed joint. CONCLUSIONS: In conclusion, inflammation and 17 beta-estradiol can modulate ERalpha expression in the TMJ but the effects are tissue specific

Expressions 1978

Expressions contains selected work from some of the Creative Writing Contest winners and honorable mentions along with Commercial Art students at Des Moines Area Community College. Design, topography, and layout was accomplished by Journalism students.https://openspace.dmacc.edu/expressions/1000/thumbnail.jp

Baker, Elmer LeRoy

"Six-Shooter Man" manuscript account of the death of Jake Hamon as told by Bob Hutchins. Hutchins had been a bodyguard to Jake and Clara Hamon and had knowledge of events surrounding the shooting. 33 pages, types, unpublished manuscrip

Who are Appalachian Males and What our Research Showed Us

Chris Green will be giving this talk to start the roundtable. Much has been made of the lower graduation rates of males in four year colleges. At Berea College we have also found this to be true, with fewer than half of African American males and males from distressed Appalachian counties both graduating in six year, almost 18% lower than the average graduation rate for the College. In Spring 2013, a team assembled to investigate these students experience on campus and the hurdles they faced. After doing so, the African American and Appalachian Male retention teams explored ways to assist the students in succeeding on campus as well as how to change aspect of campus culture. This roundtable brings together seven people involved in enacting the first stage of the Appalachian Male Initiative where 30 freshmen students from at-risk and distressed counties took a class in Appalachian Cultures that also focused on their experience on campus, both exploring and affirming their identities and exploring conflicts such as the limiting of self-direction they once had, conflicts over politics and religion, and issues such as white privilege

Estrogen and inflammation modulate estrogen receptor alpha expression in specific tissues of the temporomandibular joint

Abstract Background Estrogen is known to play role in temporomandibular joint (TMJ) disorders and estrogen effects can be mediated by estrogen receptor (ER) alpha present in the TMJ. Cells expressing the estrogen receptor ERalpha are present in the temporomandibular joint (TMJ) but changes in expression due to estrogen and inflammation have not been characterized. In this study, ERalpha protein content and the number of cells expressing ERalpha was measured in 17 beta-estradiol-treated rats after inflammation was induced in the TMJ. Methods Sixteen ovariectomized female rats were divided into two groups such that one group received 17 beta estradiol (E2) and the other was given vehicle (VEH). Groups were then subdivided further, one received injections of saline and the other received Complete Freund's adjuvant (CFA) within the superior joint space of the TMJ. Thus the four groups include no E2/saline, E2/saline, no E2/CFA and E2/CFA. After treatment, the rats were sacrificed, and the TMJ anterior, disc, retrodiscal and synovial tissues were analyzed by western blot and immunocytochemistry. Positive stained cells were counted using a Nikon epifluorescent microscope. Results The western blot showed that ERalpha protein significantly decreased with inflammation. The number of ERalpha-positive cells in the TMJ was not affected by inflammation or 17 beta-estradiol with exception of the retrodiscal tissue. In the retrodiscal tissue 17 beta-estradiol significantly decreased the number of ERalpha-positive cells but only in a non-inflamed joint. Conclusions In conclusion, inflammation and 17 beta-estradiol can modulate ERalpha expression in the TMJ but the effects are tissue specific.</p

Freeing Testers from Polluting Test Objectives

Testing is the primary approach for detecting software defects. A major challenge faced by testers lies in crafting efficient test suites, able to detect a maximum number of bugs with manageable effort. To do so, they rely on coverage criteria, which define some precise test objectives to be covered. However, many common criteria specify a significant number of objectives that occur to be infeasible or redundant in practice, like covering dead code or semantically equal mutants. Such objectives are well-known to be harmful to the design of test suites, impacting both the efficiency and precision of testers' effort. This work introduces a sound and scalable formal technique able to prune out a significant part of the infeasible and redundant objectives produced by a large panel of white-box criteria. In a nutshell, we reduce this challenging problem to proving the validity of logical assertions in the code under test. This technique is implemented in a tool that relies on weakest-precondition calculus and SMT solving for proving the assertions. The tool is built on top of the Frama-C verification platform, which we carefully tune for our specific scalability needs. The experiments reveal that the tool can prune out up to 27% of test objectives in a program and scale to applications of 200K lines of code

Strengthening national capital: A postcolonial analysis of lifelong learning policy in St Lucia, Caribbean

This paper provides a postcolonial policy analysis of the Education Sector Development Plan: 2000-2005 and Beyond in the small Caribbean island nation of St Lucia. The specific focus is upon the nature of the lifelong learning policy as incorporated in the Plan. This is shown to be a globalised policy discourse. Drawing on a number of postcolonial theorists, the paper works with a concept of the postcolonial as an aspirational politics. Bourdieu's concept of 'national capital' is also utilised in the analysis in relation to the attempt to manage some autonomy for the nation in the process of policy text production in education. The analysis demonstrates the way a postcolonial politics, manifest as strengthening national capital, worked in relation to the production of the Plan in its consultative mode of production and in its extensive cross-sector coverage. However, the analysis also shows evidence of the effects of residues of the colonial past in the hegemony of English in the Plan's recommendations, in the restriction of lifelong learning facilitation to schools and in the denial of a place for indigenous knowledges. The mode of lifelong learning supported is also dominated by a human capital framework and neglect of other capitals (social, cultural, etc.) for constituting what might be seen as a postcolonial and creole learning society. The paper also reflects upon the extent of the postcoloniality of the analysis provided