Analysis of Ethyl p-Methoxycinnamate from Kaempferia galanga L. Extract by High Performance Liquid Chromatography

Ethyl para-methoxycinamate (EPMS) is a major compound of Kaempferia galanga L that has anti-inflammatory effect.  The purpose of this study was to determine of EPMS in Kaempferiae galanga L rhizome extract by  High Performance Liquid Chromatography (HPLC) and evaluated the performance of the analysis. This study included determination of system suitability, accuracy, precision, linearity and range, limit of detection (LOD) and Limit of quantitation (LOQ) and selectivity.  The results of system suitability test  HPLC System for EPMS analysis were as follows isocratic elution system of a mobile phase mixture of methanol: water (70:30) containing 0.1% TFA, uv detector at a wavelength of 308 nm using column C18 (150 × 4, 6mm, 5?m) flow rate 1 ml / min. From the analysis, it was found that the average EPMS content was 78.74%. Then method had linear concentration range from 5-360 ppm, with R ² = 0.9999. The LOD and LOQ were 7.0722 ppm and 21.4311 ppm respectively. The accuracy of this method that represented by % recovery was 98.02% - 101.26%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 1.57%. The selectivity of this method that showed by  resolution value was 2.6. Based on the results of the system suitability test and analysis performance evaluation,all parameters met the requirements

Review: Analisis Komponen Senyawa Kimia Krokot (Portulaca oleraceae L. dan Portulaca grandiflora Hook.): Review: Component Analysis of Purslanes Chemicals Compound (Portulaca oleraceae L. andPortulaca grandiflora Hook.)

Purslanes are medicinal plants from the Portulacaceae family which has more than 100 species, including Portulaca oleraceae L. and Portulaca grandiflora Hook. Both types of purslanes have the potential to become natural medicines due to the presence of active chemical compounds that produce various pharmacological effects. This review compiled the data concerning chemical compounds found in those two types of purslanes, the method of analysis, as well as the factors that affect the quality and quantity of chemical compounds so that they can be used as references in determining natural medicinal ingredients by selecting purslanes based on their desired phytochemical compounds and the appropriate analysis. The data were collected by a method of searching research journals on Google Scholar using certain keywords. From this review, the results showed that the chemical compounds found in the two types of purslanes are phenolic, flavonoid, fatty acid, sterol, terpenoid, and alkaloid. These components can be analyzed using spectroscopic and chromatographic methods for both qualitative and quantitative analysis. Some factors that need to be considered in the analysis of these components are the analytical method used, the method of extraction, the solvents, the plant varieties and genotypes, the part of the plant used, the growth time and harvest time of the sample, and the conditions in which the samples grew

Pengaruh Tempat Tumbuh Terhadap Kadar Alfa Tokoferol Pada Buah Mengkudu (Morinda Citrifolia L.): The Effect of Location on Alfa Tocopherol Levels in Noni Fruit (Morinda Citrifolia L.)

Alpha tocopherol (?-tocopherol) contained in noni fruit (Morinda citrifolia L.) is one of the antioxidants that contribute to pharmacological effects. The noni plant is a tropical plant that has broad growing requirements, so that this plant can grow anywhere. Although easy to find, the environmental conditions where noni grows will have an influence on the amount of ?-tocopherol it contains, one of which is the difference in where it grows. The purpose of this study was to determine the levels of ?-tocopherol in the n-hexane fraction of noni fruit, which grows in three different growing places. Analysis for ?-tocopherol by High Performance Liquid Chromatography (HPLC) with a validated analytical method. The results of system suitability test HPLC System for ?- tocopherol analysis were as follows isocratic elution system of a mobile phase 100% methanol, uv detector at a wavelength of 292 nm using the column C18 (150 ? 4, 6mm, 5?m) flow rate of 1 ml / min. The method had linear concentration range from 1-25 ppm, with R2 0.9967. The accuracy of this method that represented by recovery was 98.02-99.252%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 0.262% and has good specificity. From the analysis, it was found that the content of ?-tocopherol in noni fruit was different and had a positive correlation with the height where it grew, namely in Pameungpeuk (18 m asl) of 0.0316%; Kiaracondong (173 m asl) of 0.0637%; and Lembang (1200 m asl) at 1.0853%