Radio Astronomy in LSST Era

A community meeting on the topic of "Radio Astronomy in the LSST Era" was hosted by the National Radio Astronomy Observatory in Charlottesville, VA (2013 May 6--8). The focus of the workshop was on time domain radio astronomy and sky surveys. For the time domain, the extent to which radio and visible wavelength observations are required to understand several classes of transients was stressed, but there are also classes of radio transients for which no visible wavelength counterpart is yet known, providing an opportunity for discovery. From the LSST perspective, the LSST is expected to generate as many as 1 million alerts nightly, which will require even more selective specification and identification of the classes and characteristics of transients that can warrant follow up, at radio or any wavelength. The LSST will also conduct a deep survey of the sky, producing a catalog expected to contain over 38 billion objects in it. Deep radio wavelength sky surveys will also be conducted on a comparable time scale, and radio and visible wavelength observations are part of the multi-wavelength approach needed to classify and understand these objects. Radio wavelengths are valuable because they are unaffected by dust obscuration and, for galaxies, contain contributions both from star formation and from active galactic nuclei. The workshop touched on several other topics, on which there was consensus including the placement of other LSST "Deep Drilling Fields," inter-operability of software tools, and the challenge of filtering and exploiting the LSST data stream. There were also topics for which there was insufficient time for full discussion or for which no consensus was reached, which included the procedures for following up on LSST observations and the nature for future support of researchers desiring to use LSST data products.Comment: Conference summary, 29 pages, 1 figure; to be published in the Publ. Astron. Soc. Pacific; full science program and presentations available at http://science.nrao.edu/science/event/RALSST201

Dual ankyrinG and subpial autoantibodies in a man with well-controlled HIV infection with steroid-responsive meningoencephalitis: A case report

Neuroinvasive infection is the most common cause of meningoencephalitis in people living with human immunodeficiency virus (HIV), but autoimmune etiologies have been reported. We present the case of a 51-year-old man living with HIV infection with steroid-responsive meningoencephalitis whose comprehensive pathogen testing was non-diagnostic. Subsequent tissue-based immunofluorescence with acute-phase cerebrospinal fluid revealed anti-neural antibodies localizing to the axon initial segment (AIS), the node of Ranvier (NoR), and the subpial space. Phage display immunoprecipitation sequencing identified ankyrinG (AnkG) as the leading candidate autoantigen. A synthetic blocking peptide encoding the PhIP-Seq-identified AnkG epitope neutralized CSF IgG binding to the AIS and NoR, thereby confirming a monoepitopic AnkG antibody response. However, subpial immunostaining persisted, indicating the presence of additional autoantibodies. Review of archival tissue-based staining identified candidate AnkG autoantibodies in a 60-year-old woman with metastatic ovarian cancer and seizures that were subsequently validated by cell-based assay. AnkG antibodies were not detected by tissue-based assay and/or PhIP-Seq in control CSF (N = 39), HIV CSF (N = 79), or other suspected and confirmed neuroinflammatory CSF cases (N = 1,236). Therefore, AnkG autoantibodies in CSF are rare but extend the catalog of AIS and NoR autoantibodies associated with neurological autoimmunity

Dual ankyrinG and subpial autoantibodies in a man with well-controlled HIV infection with steroid-responsive meningoencephalitis: A case report

Neuroinvasive infection is the most common cause of meningoencephalitis in people living with human immunodeficiency virus (HIV), but autoimmune etiologies have been reported. We present the case of a 51-year-old man living with HIV infection with steroid-responsive meningoencephalitis whose comprehensive pathogen testing was non-diagnostic. Subsequent tissue-based immunofluorescence with acute-phase cerebrospinal fluid revealed anti-neural antibodies localizing to the axon initial segment (AIS), the node of Ranvier (NoR), and the subpial space. Phage display immunoprecipitation sequencing identified ankyrinG (AnkG) as the leading candidate autoantigen. A synthetic blocking peptide encoding the PhIP-Seq-identified AnkG epitope neutralized CSF IgG binding to the AIS and NoR, thereby confirming a monoepitopic AnkG antibody response. However, subpial immunostaining persisted, indicating the presence of additional autoantibodies. Review of archival tissue-based staining identified candidate AnkG autoantibodies in a 60-year-old woman with metastatic ovarian cancer and seizures that were subsequently validated by cell-based assay. AnkG antibodies were not detected by tissue-based assay and/or PhIP-Seq in control CSF (N = 39), HIV CSF (N = 79), or other suspected and confirmed neuroinflammatory CSF cases (N = 1,236). Therefore, AnkG autoantibodies in CSF are rare but extend the catalog of AIS and NoR autoantibodies associated with neurological autoimmunity