A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets

The sexes of non-ratite birds can be determined routinely by PCR amplification of the CHD-Z and CHD-W genes. CHD -based molecular sexing of four species of auklets revealed the presence of a polymorphism in the Z chromosome. No deviation from a 1:1 sex ratio was observed among the chicks, though the analyses were of limited power. Polymorphism in the CHD-Z gene has not been reported previously in any bird, but if undetected it could lead to the incorrect assignment of sex. We discuss the potential difficulties caused by a polymorphism such as that identified in auklets and the merits of alternative CHD -based sexing protocols and primers

30 Doradus - a Template for "Real Starbursts"?

30 Doradus is the closest massive star forming region and the best studied template of a starburst. In this conference paper we first summarize the properties of 30 Doradus and its stellar core, R136. We discuss the effects of insufficient spatial resolution and cluster density profiles on dynamical mass estimates of super star clusters, and show that their masses can be easily overestimated by a factor of ten or more. From a very simple model, with R136-like clusters as representative building blocks, we estimate typical luminosities of the order 10^11 L_o for starburst galaxies.Comment: To be published in "Starbursts: From 30 Doradus to Lyman Break Galaxies", eds. R. de Grijs & R.M. Gonzalez Delgad

The Maximum Mass of Star Clusters

When an universal untruncated star cluster initial mass function (CIMF) described by a power-law distribution is assumed, the mass of the most massive star cluster in a galaxy (M_max) is the result of the size-of-sample (SoS) effect. This implies a dependence of M_max on the total number of star clusters (N). The SoS effect also implies that M_max within a cluster population increases with equal logarithmic intervals of age. This is because the number of clusters formed in logarithmic age intervals increases (assuming a constant cluster formation rate). This effect has been observed in the SMC and LMC. Based on the maximum pressure (P_int) inside molecular clouds, it has been suggested that a physical maximum mass (M_max[phys]) should exist. The theory predicts that M_max[phys] should be observable, i.e. lower than M_max that follows from statistical arguments, in big galaxies with a high star formation rate. We compare the SoS relations in the SMC and LMC with the ones in M51 and model the integrated cluster luminosity function (CLF) for two cases: 1) M_max is determined by the SoS effect and 2) M_max=M_max[phys]=constant. The observed CLF of M51 and the comparison of the SoS relations with the SMC and LMC both suggest that there exists a M_max[phys] of 5*10^5 M_sun in M51. The CLF of M51 looks very similar to the one observed in the ``Antennae'' galaxies. A direct comparison with our model suggests that there M_max[phys]=2*10^6 M_sun.Comment: 4 pages, contribution to "Globular Clusters: Guides to Galaxies", March 6th-10th, 200

Outskirts of Nearby Disk Galaxies: Star Formation and Stellar Populations

The properties and star formation processes in the far-outer disks of nearby spiral and dwarf irregular galaxies are reviewed. The origin and structure of the generally exponential profiles in stellar disks is considered to result from cosmological infall combined with a non-linear star formation law and a history of stellar migration and scattering from spirals, bars, and random collisions with interstellar clouds. In both spirals and dwarfs, the far-outer disks tend to be older, redder and thicker than the inner disks, with the overall radial profiles suggesting inside-out star formation plus stellar scattering in spirals, and outside-in star formation with a possible contribution from scattering in dwarfs. Dwarf irregulars and the far-outer parts of spirals both tend to be gas dominated, and the gas radial profile is often non-exponential although still decreasing with radius. The ratio of H-alpha to far-UV flux tends to decrease with lower surface brightness in these regions, suggesting either a change in the initial stellar mass function or the sampling of that function, or a possible loss of H-alpha photons.Comment: 20 pages, 8 figures, Invited review, Book chapter in "Outskirts of Galaxies", Eds. J. H. Knapen, J. C. Lee and A. Gil de Paz, Astrophysics and Space Science Library, Springer, in pres

II Zwicky 23 and Family

II Zwicky 23 (UGC 3179) is a luminous, nearby compact narrow emission line starburst galaxy with blue optical colors and strong emission lines. We present a photometric and morphological study of II Zw 23 and its interacting companions using data obtained with the WIYN 3.5-m telescope in Kitt Peak, Arizona. II Zwicky 23 has a highly disturbed outer structure with long trails of debris that may be feeding tidal dwarfs. Its central regions appear disky, a structure that is consistent with the overall rotation pattern observed in the H-alpha velocity field measured from Densepak observations obtained with WIYN. We discuss the structure of II Zwicky 23 and its set of companions and possible scenarios of debris formation in this system.Comment: 5 pages, 2 figures. To appear in the proceedings of ESO Astrophysics Symposia: "Groups of Galaxies in the Nearby Universe", eds. I. Saviane, V. Ivanov, J. Burissova (Springer

An ethical analysis of coverage with evidence development

Sometimes a government or other payer is called on to fund a new health technology even when the evidence leaves a lot of uncertainty. One way to manage this problem is for the payer to provisionally fund the technology and reduce uncertainty by developing evidence. This is referred to as coverage with evidence development (CED). When the payer funds the technology only for patients who participate in the evidence development, the coverage is known as only-in-research. This type of CED raises the sharpest questions of coercion and inducement. Is the patient coerced or induced into participating in the evidence development? If so, under what circumstances, if any, is this ethically justified? Building on work by Miller and Pearson, we argue that patients have a right to funding for a technology only when the payer can be confident that the technology provides reasonable value for money. This means that patients have no right to technologies under a CED arrangement, since technologies are candidates for CED precisely because serious questions remain about value for money. It is for this reason that CED induces rather than coerces. The separate question of whether the inducement is ethically justified remains. We argue that CED does pose risks to patients, and the worse these risks are, the more difficult it is to justify the inducement. Finally, we propose conditions under which the inducement could be ethically justified, as well as means of avoiding inducement altogether. All of this has major bearings on the types of CED that are ethically justified and the governance structures that need to be in place. To develop our argument, we draw on the Australian context, so our conclusions apply most directly to comparable contexts, where the payer is a government that provides universal coverage with a regard for cost effectiveness that is prominent and fairly clearly defined.Drew Carter, Tracy Merlin, David Hunte

The influence of fall and spring herbicide application on winter wheat

Non-Peer ReviewedThe use of 2,4-D for fall control of winter annuals weeds in winter wheat is recommended in the Canadian prairies but not in other winter wheat production areas. Field research was conducted for two years at several locations using 2,4-0, MCPA, dicamba, dicamba plus 2,4-D, bromoxynil, bromoxynil plus MCPA, chlorsulfuron and clopyralid applied fall and spring. Recommended and double recommended rates were tested to establish the existence of a safety margin. Norstar winter wheat was tolerant to fall applications of all herbicides when applied at recommended rates, however, grain yield was reduced at the double rate with 2,4-D, MCPA, bromoxynil, bromoxynil plus MCPA and clopyralid in some station years. Norstar was tolerant to spring applications of all herbicides except MCPA and clopyralid in several station years. Herbicides tested show promise for use in winter wheat production. Caution is warranted for spring application of MCPA and clopyralid

The ChlD subunit links the motor and porphyrin binding subunits of magnesium chelatase

Magnesium chelatase initiates chlorophyll biosynthesis, catalysing the MgATP2- dependent insertion of a Mg2+ ion into protoporphyin IX. The catalytic core of this large enzyme complex consists of three subunits: Bch/ChlI, Bch/ChlD and Bch/ChlH (in bacteriochlorophyll and chlorophyll producing species respectively). The D and I subunits are members of the AAA+ (ATPases associated with various cellular activities) superfamily of enzymes, and they form a complex that binds to H, the site of metal ion insertion. In order to investigate the physical coupling between ChlID and ChlH in vivo and in vitro , ChlD was FLAG-tagged in the cyanobacterium Synechocystis sp. PCC 6803 and co-immunoprecipitation experiments showed interactions with both ChlI and ChlH. Co-production of recombinant ChlD and ChlH in Escherichia coli yielded a ChlDH. Quantitative analysis using microscale thermophoresis (MST) showed magnesium-dependent binding ( K d 331 ± 58 nM) between ChlD and H. The physical basis for a ChlD-H interaction was investigated using chemical crosslinking coupled with mass spectrometry (XL-MS), together with modifications that either truncate ChlD or modify single residues. We found that the C-terminal integrin I domain of ChlD governs association with ChlH, the Mg2+ dependence of which also mediates the cooperative response of the Synechocystis chelatase to magnesium. Our work, showing the interaction site between the AAA+ motor and the chelatase domain of magnesium chelatase, will be essential for understanding how free energy from the hydrolysis of ATP on the AAA+ ChlI subunit is transmitted via the bridging subunit ChlD to the active site on ChlH