Intracellular Internalization and Signaling Pathways Triggered by the Large Subunit of HSV-2 Ribonucleotide Reductase (ICP10)

AbstractThe large subunit of the HSV-2 ribonucleotide reductase (RR) (ICP10) is a chimera consisting of a serine threonine (Ser/Thr) protein kinase domain at the amino terminus and the RR domain at the carboxy terminus. Transformed human cells that constitutively express ICP10 (JHLa1) were stained with anti-LA-1 antibody (recognizes ICP10 amino acids 13-26) and immunogold-conjugated goat anti-rabbit IgG and were examined by electron microscopy. ICP10-associated gold particles were observed on the cell surface and in structures with ultrastructural characteristics of endocytic vesicles, multivesicular bodies, and lysosomes, consistent with endocytic internalization. ICP10 was also associated with the cytoskeleton fraction of JHLa1 cells and, at least in part, it colocalized with actin filaments. This was evidenced by immunoprecipitation of [35S]methionine-labeled cell fractions and immunofluorescent staining of Triton-treated cells with anti-LA-1 antibody and phalloidin. Endocytic localization of gold particles was not seen in cells that constitutively express the ICP10 transmembrane (TM)-deleted mutant p139TM (JHL15). p139TM did not associate with the cytoskeleton and was almost entirely localized within the cytoplasm, raf and Erk evidenced decreased mobility consistent with an activated state in JHLa1, but not JHL15, cells, and chloramphenicol acetyl transferase (CAT) expression from a c-fos/cat hybrid construct was significantly increased in JHLa1 but not JHL15 cells. The data indicate that effector molecules downstream of ras are activated in JHLa1 cells and the ICP10 TM segment plays a critical role in ICP10 intracellular localization and its ability to activate signaling pathways. This behavior is analogous to that of an activated growth factor receptor kinase

Search for long-lived neutral particles in pp collisions at s√=13 TeV that decay into displaced hadronic jets in the ATLAS calorimeter

This paper describes a search for pairs of neutral, long-lived particles decaying in the ATLAS calorimeter. Long-lived particles occur in many extensions to the Standard Model and may elude searches for new promptly decaying particles. The analysis considers neutral, long-lived scalars with masses between 5 and 400 GeV, produced from decays of heavy bosons with masses between 125 and 1000 GeV, where the long-lived scalars decay into Standard Model fermions. The analysis uses either 10.8 fb−1 or 33.0 fb−1 of data (depending on the trigger) recorded in 2016 at the LHC with the ATLAS detector in proton–proton collisions at a centre-of-mass energy of 13 TeV. No significant excess is observed, and limits are reported on the production cross section times branching ratio as a function of the proper decay length of the long-lived particles

Correlação entre os estágios de mineralização dentária na arcada inferior e a idade esquelética: revisão da literatura Correlationship between the stages of tooth mineralization on the lower arch and the skeletal age: literature review

O objetivo do presente trabalho foi o de revisar a literatura buscando uma correlação entre os estágios de mineralização dentária e a idade esquelética. Enquanto alguns trabalhos não encontraram nenhuma correlação entre essas idades biológicas, outros mostraram uma íntima relação entre elas, sugerindo a determinação da idade esquelética a partir da mineralização radicular de dentes específicos, principalmente no arco inferior. Portanto, um levantamento minucioso das opiniões acerca do assunto poderá colaborar para uma melhor compreensão das controvérsias existentes.<br>The aim of this work was to get a correlationship between the stages of tooth mineralization and the skeletal age. This work was carried on in order to clean the controverses find in the literature about this subject. While some articles don’t get any correlationship between those biological age, some show a great relation between them, suggesting the possibility of determine the skeletal age using the stages of tooth mineralization