1 research outputs found
A Rapid SNAP-Tag Fluorogenic Probe Based on an Environment-Sensitive Fluorophore for No-Wash Live Cell Imaging
One major limitation of labeling
proteins with synthetic fluorophores
is the high fluorescence background, which necessitates extensive
washing steps to remove unreacted fluorophores. In this paper, we
describe a novel fluorogenic probe based on an environment-sensitive
fluorophore for labeling with SNAP-tag proteins. The probe exhibits
dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag.
The major advantages of our fluorogenic probe are the dramatic fluorescence
turn-on, ease of synthesis, high selectivity, and rapid labeling with
SNAP-tag. No-wash labeling of both intracellular and cell surface
proteins was successfully achieved in living cells, and the localization
of these proteins was specifically visualized