Automated injection of slurry samples in flow-injection analysis

Two types of injectors are described for introducing solid samples as slurries in flow analysis systems. A time-based and a volume-based injector based on multitube solenoid pinch valves were built, both can be characterized as hydrodynamic injectors. Reproducibility of the injections of dispersed solids ( 150 ¿m) was tested with several concentrations of slurry samples up to 30 mg/ml; the injected volume was 1 ml. For both injectors dye and slurry samples could be injected with good precision (relative standard deviation for the peak area less than 2%). Peak detection was performed turbidimetrically. Data analysis and operation of the injectors were automated. The usual peristaltic pumps in flow analysis are normally not capable of handling slurries of the type investigated, therefore a valveless piston pump was used instead

Guilt in the aftermath of killing in close combat, guilt as an endorsement of one‟s essential goodness?

This thesis fills in gaps within research into the psychological and emotional consequences of combat by addressing the question ‘What is the role of guilt in coping with killing a military opponent at close range?’. The focus is on killing and guilt: psychological and psychosocial processes, communication and reflections on killing. The data is based on in-depth interviews with veterans in London who have killed in combat. Guilt has the function of guiding moral behaviour. The exercise of personal conscience, or feeling guilty about killing, could undermine the drive to kill and seriously threaten an entire military enterprise. However, by having some feelings of guilt, a modern soldier feels he is a responsible and conscientious person. This thesis is also a moral appeal to politicians, mental health workers and ordinary citizens alike to think about how we look at soldiers who fight and kill in wars that politicians - on our behalf - chose to start or to participate in. Society has a responsibility to help soldiers with the after effects of killing on our behalf. This thesis offers an insight into how soldiers construct their experience of killing an opponent and offers recommendations on how to help soldiers in coping with and managing these experiences, suggesting improvements to after care are needed

Vagus Nerve Stimulation in Refractory Epilepsy: Effects on Pro- and Anti-Inflammatory Cytokines in Peripheral Blood

Objective: The vagus nerve has important immunological functions that may be relevant for its anticonvulsive action. We postulate that this anticonvulsive action is activated by a shift in the immune system resulting in a reduction of neurotoxic and an increase of neuroprotective tryptophan metabolites. Methods: Eleven patients with refractory epilepsy and 11 controls matched for age and gender were included in this study. The primary outcome measure was a 50% seizure reduction. Other variables were pro-inflammatory cytokines IL-6 and TNF-alpha, anti-inflammatory cytokine IL-10, cortisol, and the tryptophan metabolites 3-hydroxykynurenine (3-OH-KYN), kynurenic acid (KYNA), kynurenine, serotonin (5-HT) and 5-hydroxyindol acetic acid (5-HIAA). Blood samples were scheduled during baseline, and in week 28 of add-on treatment. Results: IL-6 levels were higher in the responders than in the control group, and decreased after vagus nerve stimulation (VNS), whereas IL-10 was low and increased after VNS. In nonresponders, VNS resulted in an increase of IL-6 plasma levels and in a decrease of IL-10. Cortisol concentrations are higher in the epilepsy group than in the control group. After VNS, these concentrations decreased. The concentrations of the tryptophan metabolites were lower in the epilepsy group than in the control group. The KYNA ratios are defined as the ratio of neuroprotective KYNA versus neurotoxic 3-OH-KYN and KYNA versus neurotoxic kynurenine: these ratios were lower in epilepsy patients than in controls, and they both moderately increased after VNS. Conclusion: The outcome of this preliminary study indicates that VNS causes a rebalancing of the immune system. This results in: (1) a reduction of neurotoxic and an increase of neuroprotective kynurenine metabolites and (2) in the normalization of cortisol levels. Copyright (C) 2010 S. Karger AG, Base

Maximizing Use of an Extension Beef Cattle Data Set: Part 2—Reproductive Rates

Previously, we described CHAPS20Y, a historical data set, and trends in CHAPS20Y calving distributions. In this article, we describe reproductive rates, including total females exposed to bull(s), pregnancy, pregnancy loss, calving, calf death loss, weaning, culling, and replacement percentages. Yearly mean exposed females increased from 1994 through 2013, consistent with a greater replacement percentage versus culling percentage. Yearly mean reproductive percentages varied over time. Factors such as weather, body condition, temperament, nutrition, and health likely contributed to this variation. CHAPS20Y is a tool Extension professionals can use to understand beef reproductive rates and help producers set and achieve their herd management goals

Maximizing Use of Extension Beef Cattle Benchmarks Data Derived from Cow Herd Appraisal Performance Software

One goal of Extension is to provide practical information that makes a difference to producers. Cow Herd Appraisal Performance Software (CHAPS) has provided beef producers with production benchmarks for 30 years, creating a large historical data set. Many such large data sets contain useful information but are underutilized. Our goal was to create a 20-year data set (CHAPS20Y) to examine trends in beef production from 1994 to 2013. In this article, we describe the CHAPS program and the process used to select herds for CHAPS20Y. We hope to publish additional related articles that will examine trends in calving distributions, reproduction, and growth and discuss implications for producers and Extension

Effect of introduced species and habitat alteration on the occurrence and distribution of euryhaline fishes in fresh- and brackish-water habitats on Aruba, Bonaire and Curaçao (South Caribbean)

We conducted an ichthyological survey during the dry season of 2006 on the semi-arid islands of Aruba, Bonaire and Curaçao to provide information on species composition, richness and distribution in natural and non-natural aquatic habitats. The dry season species assemblages (N = 9 species) comprised less species than the wet seasons, and these data refine our knowledge of the indigenous fish fauna and its refuge localities during phases of drought and ensuing high salinity. A hierarchical cluster analysis reveals that the three islands have different species compositions with Curaçao being the most diverse, probably due to its having the most habitats and freshwaters present throughout the year. Species richness was unrelated to salinity and species diversity was highest in canalised streams. In the dry season fewer amphidromous species are present than in the wet season. We found no significant effect of human-induced changes on the presence or absence of fish species in the Netherlands Antilles. The presence of exotic species (including Xiphophorus helleri on Aruba, a first record for this island, and Oreochromis mossambicus and Poecilia reticulata occurring on all three islands) did not have a clear effect on the presence of indigenous species, nor did human alteration of the habitats have an influence on the occurrence of fish species

Ezh2 inhibition in Kras-driven lung cancer amplifies inflammation and associated vulnerabilities

Kras-driven non–small-cell lung cancers (NSCLCs) are a leading cause of death with limited therapeutic options. Many NSCLCs exhibit high levels of Ezh2, the enzymatic subunit of polycomb repressive complex 2 (PRC2). We tested Ezh2 inhibitors as single agents or before chemotherapy in mice with orthotopic Kras-driven NSCLC grafts, which homogeneously express Ezh2. These tumors display sensitivity to EZH2 inhibition by GSK126 but also amplify an inflammatory program involving signaling through NF-κB and genes residing in PRC2-regulated chromatin. During this process, tumor cells overcome GSK126 antiproliferative effects. We identified oncogenes that may mediate progression through an in vivo RNAi screen aimed at targets of PRC2/NF-κB. An in vitro compound screening linked GSK126-driven inflammation and therapeutic vulnerability in human cells to regulation of RNA synthesis and proteostasis. Interestingly, GSK126-treated NSCLCs in vivo also showed an enhanced response to a combination of nimesulide and bortezomib. Thus, Ezh2 inhibition may restrict cell proliferation and promote defined adaptive responses. Targeting these responses potentially improves outcomes in Kras-driven NSCLCs