MOESM2 of PBMC fixation and processing for Chromium single-cell RNA sequencing

Additional file 2: Table S2. Marker genes used for identifying PBMC subpopulations

MOESM3 of PBMC fixation and processing for Chromium single-cell RNA sequencing

Additional file 3: Figure S1. Resuspension in ×3 SSC preserved cell RNA integrity. a. The methanol-fixed PBMCs resuspended in ×3 or ×5 SSC buffer showed high quality of RNA determined with Bioanalyzer traces. b. The new processing method was validated in several other cell types resuspended in ×3 SSC for 30 min. The RIN numbers were significantly improved in primary cell types and cell lines with a p value of 0.00001 and 0.008 respectively

MOESM6 of PBMC fixation and processing for Chromium single-cell RNA sequencing

Additional file 6: Figure S3. tSNE projection of live and fixed PBMCs from donor DTM-X (a) and donor (b). Cells were grouped using graph-based method. Classification of PBMCs was inferred from the annotation of cluster-specific genes, and based on expression of some well-known markers of immune cell types. Although fixation lead to changes of the relative distances of the clusters due to the loss of genes detected, it did not impact the resolution of the low abundant populations (B, NK, DC) in each sample. Subpopulations were detected from fixed PBMCs at a similar proportion to those of live PBMCs (Table 1)

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Additional file 5: Table S3. Sequencing metrics summary of 10 scRNA-Seq datasets

MOESM1 of Impaired B cell immunity in acute myeloid leukemia patients after chemotherapy

Additional file 1. Supplemental methods