A study of the problems associated with Dalangdian reservoir, China

There are over 2,300 lakes over 1 km2 in China (total area 80 000 km2). In addition there are approximately 87 000 reservoirs with a storage capacity of 413 billion m3. These form the main supply of drinking water as well as water for industrial and agricultural production and aquaculture. Because of a lack of understanding of the frailty of lake ecosystems and poor environmental awareness, human activities have greatly affected freshwater systems. This article focuses on the problems of one water supply reservoir, Dalangdian Reservoir, and considers options for improving its management. Dalangdian Reservoir is described and occurrence of algal genera given. The authors conclude with remarks on the future of the Dalangdian Reservoir

Blacklight: Defending Black-Box Adversarial Attacks on Deep Neural Networks

The vulnerability of deep neural networks (DNNs) to adversarial examples is well documented. Under the strong white-box threat model, where attackers have full access to DNN internals, recent work has produced continual advancements in defenses, often followed by more powerful attacks that break them. Meanwhile, research on the more realistic black-box threat model has focused almost entirely on reducing the query-cost of attacks, making them increasingly practical for ML models already deployed today. This paper proposes and evaluates Blacklight, a new defense against black-box adversarial attacks. Blacklight targets a key property of black-box attacks: to compute adversarial examples, they produce sequences of highly similar images while trying to minimize the distance from some initial benign input. To detect an attack, Blacklight computes for each query image a compact set of one-way hash values that form a probabilistic fingerprint. Variants of an image produce nearly identical fingerprints, and fingerprint generation is robust against manipulation. We evaluate Blacklight on 5 state-of-the-art black-box attacks, across a variety of models and classification tasks. While the most efficient attacks take thousands or tens of thousands of queries to complete, Blacklight identifies them all, often after only a handful of queries. Blacklight is also robust against several powerful countermeasures, including an optimal black-box attack that approximates white-box attacks in efficiency. Finally, Blacklight significantly outperforms the only known alternative in both detection coverage of attack queries and resistance against persistent attackers

A study of the problems associated with Dalangdian reservoir, China

There are over 2,300 lakes over 1 km2in China (total area 80 000 km2,accounting for about 0.8% of the total area of the country and with a totalfreshwater storage capacity of 225 billion m3). In addition there areapproximately 87 000 reservoirs with a storage capacity of 413 billion m3.These form the main supply of drinking water as well as water for industrialand agricultural production and aquaculture. Because of a lack ofunderstanding of the frailty of lake ecosystems and poor environmentalawareness, human activities have greatly affected freshwater systems (Xie1995). The most marked impacts have been due to land reclamation,destruction of marginal vegetation, discharge of industrial, domestic andagricultural waste and irrational development and use of freshwaterresources. As a result lakes in dry areas are becoming saline, while othersare highly polluted and undergoing a process of rapid eutrophication. Themajority of the lakes tested to date have been classified as hypertrophic(Dokulil et al. 2000). Thus, although China has 28 of the world's largestlakes (Chang 1987, Chen 1994), the annual storage capacity ranks sixth inthe world and the per capita availability is a quarter of the world's average(Jusi 1989) and predicted to fall by the year 2000 (Shiklomanov 1993). Inthis article, we focus on the problems of one water supply reservoir

Ezrin interacts with the SARS coronavirus spike protein and restrains infection at the entry stage

© 2012 Millet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background: Entry of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and its envelope fusion with host cell membrane are controlled by a series of complex molecular mechanisms, largely dependent on the viral envelope glycoprotein Spike (S). There are still many unknowns on the implication of cellular factors that regulate the entry process. Methodology/Principal Findings: We performed a yeast two-hybrid screen using as bait the carboxy-terminal endodomain of S, which faces the cytosol during and after opening of the fusion pore at early stages of the virus life cycle. Here we show that the ezrin membrane-actin linker interacts with S endodomain through the F1 lobe of its FERM domain and that both the eight carboxy-terminal amino-acids and a membrane-proximal cysteine cluster of S endodomain are important for this interaction in vitro. Interestingly, we found that ezrin is present at the site of entry of S-pseudotyped lentiviral particles in Vero E6 cells. Targeting ezrin function by small interfering RNA increased S-mediated entry of pseudotyped particles in epithelial cells. Furthermore, deletion of the eight carboxy-terminal amino acids of S enhanced S-pseudotyped particles infection. Expression of the ezrin dominant negative FERM domain enhanced cell susceptibility to infection by SARS-CoV and S pseudotyped particles and potentiated S-dependent membrane fusion. Conclusions/Significance: Ezrin interacts with SARS-CoV S endodomain and limits virus entry and fusion. Our data present a novel mechanism involving a cellular factor in the regulation of S-dependent early events of infection.This work was supported by the Research Grant Council of Hong Kong (RGC#760208)and the RESPARI project of the International Network of Pasteur Institutes

Cell Membrane Is Impaired, Accompanied by Enhanced Type III Secretion System Expression in Yersinia pestis Deficient in RovA Regulator

BACKGROUND: In the enteropathogenic Yersinia species, RovA regulates the expression of invasin, which is important for enteropathogenic pathogenesis but is inactivated in Yersinia pestis. Investigation of the RovA regulon in Y. pestis at 26 °C has revealed that RovA is a global regulator that contributes to virulence in part by the direct regulation of psaEFABC. However, the regulatory roles of RovA in Y. pestis at 37 °C, which allows most virulence factors in mammalian hosts to be expressed, are still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: The transcriptional profile of an in-frame rovA mutant of Y. pestis biovar Microtus strain 201 was analyzed under type III secretion system (T3SS) induction conditions using microarray techniques, and it was revealed that many cell-envelope and transport/binding proteins were differentially expressed in the ΔrovA mutant. Most noticeably, many of the T3SS genes, including operons encoding the translocon, needle and Yop (Yersinia outer protein) effectors, were significantly up-regulated. Analysis of Yop proteins confirmed that YopE and YopJ were also expressed in greater amounts in the mutant. However, electrophoresis mobility shift assay results demonstrated that the His-RovA protein could not bind to the promoter sequences of the T3SS genes, suggesting that an indirect regulatory mechanism is involved. Transmission electron microscopy analysis indicated that there are small loose electron dense particle-like structures that surround the outer membrane of the mutant cells. The bacterial membrane permeability to CFSE (carboxyfluorescein diacetate succinimidyl ester) was significantly decreased in the ΔrovA mutant compared to the wild-type strain. Taken together, these results revealed the improper construction and dysfunction of the membrane in the ΔrovA mutant. CONCLUSIONS/SIGNIFICANCE: We demonstrated that the RovA regulator plays critical roles in the construction and functioning of the bacterial membrane, which sheds considerable light on the regulatory functions of RovA in antibiotic resistance and environmental adaptation. The expression of T3SS was upregulated in the ΔrovA mutant through an indirect regulatory mechanism, which is possibly related to the altered membrane construction in the mutant

Nanostructures Technology, Research, and Applications

Contains reports on seventeen research projects and a list of publications.Joint Services Electronics Program Contract DAAL03-92-C-0001Joint Services Electronics Program Grant DAAH04-95-1-0038Semiconductor Research Corporation Contract 94-MJ-550National Science Foundation Grant ECS 94-07078U.S. Army Research Office Contract DAAL03-92-G-0291Advanced Research Projects Agency/Naval Air Systems Command Contract N00019-92-K-0021National Aeronautics and Space Administration Contract NAS8-36748National Aeronautics and Space Administration Grant NAGW-2003IBM Corporation Contract 1622U.S. Army Research Office Grant DAAH04-94-G-0377U.S. Air Force - Office of Scientific Research Grant F-49-620-92-J-006