QTL mapping of selenium content using a RIL population in wheat

<div><p>Selenium (Se) is an essential trace element that plays various roles in human health. Understanding the genetic control of Se content and quantitative trait loci (QTL) mapping provide a basis for Se biofortification of wheat to enhance grain Se content. In the present study, a set of recombinant inbred lines (RILs) derived from two Chinese winter wheat varieties (Tainong18 and Linmai6) was used to detect QTLs for Se content in hydroponic and field trials. In total, 16 QTLs for six Se content-related traits were detected on eight chromosomes, 1B, 2B, 4B, 5A, 5B, 5D, 6A, and 7D. Of these, seven QTLs were detected at the seedling stage and nine at the adult stage. The contribution of each QTL to Se content ranged from 7.37% to 20.22%. <i>QSsece-7D</i>.<i>2</i>, located between marker loci D-3033829 and D-1668160, had the highest contribution (20.22%). This study helps in understanding the genetic basis for Se contents and will provide a basis for gene mapping of Se content in wheat.</p></div

Additive QTLs for Se efficiency traits at seedling stage under different Se treatments in hydroponic culture.

<p>Additive QTLs for Se efficiency traits at seedling stage under different Se treatments in hydroponic culture.</p

Chromosomal locations of 16 QTLs for the investigated traits based on RILs derived from the cross “TN18 × LM6”.

<p>(A) QTLs are indicated on the right side of each chromosome. LOD threshold was set at 3.0. (B) QRsece, QSsece, QRsec, QSsec, QGsece, and QGsec are QTLs for root Se concentration, shoot Se concentration, root Se content per plant, shoot Se content per plant, grain Se concentration, and grain Se content per plant, respectively.</p

ANOVA results of the investigated traits.

<p>ANOVA results of the investigated traits.</p

Summary statistics of the phenotypic performance for Se content for the RILs and their parents.

<p>Summary statistics of the phenotypic performance for Se content for the RILs and their parents.</p