The effect of sodium and carbohydrate in a rehydration food on subsequent exercise performance

The purpose of this study was to determine the effectiveness of two rehydration beverages on fluid restoration and metabolism on subsequent exercise following exercise- and heat-induced dehydration. Twelve male subjects were dehydrated by ~3 % of body mass with exercise and heat exposure. In a randomly assigned, counterbalanced design, subjects were rehydrated at room temperature with either 175 ml of chicken noodle soup (SOUP: Campbell Soup Company, Camden, NJ) or an artificially sweetened placebo (CON) at the beginning of the 120 min rehydration period and 20 min later. Water was ingested every 20 min during the remaining 100 min of rehydration. Soup contained 161.0 mmol/l Na+, 5.3 mmol/l K+, and 32.6 g total carbohydrate. CON contained 14.4 mmol/l Na+, 16.0 mmol/l K+, and no carbohydrate. Total fluid ingestion was matched with body fluid loss during dehydration. After rehydration, subjects performed 30 min of steady state exercise at 68% VO2peak. Subjects then performed a time trial in which they accumulated as rapidly as possible the amount of work equal to 30 min of exercise at 70% VO2peak in a thermo-neutral environment (25 C and 40% relative humidity). There was no significant difference in percent recovery of body mass during rehydration (CON: 79.4 y 3.3%; SOUP: 80.9 y 3.4%). Time trial performance was significantly improved in SOUP (30.6 y 0.8 min) compared with CON (33.2 y 1.4 min; p=0.031). The rate of carbohydrate oxidation tended to be higher in SOUP (2.33 y 0.09 g/min) compared with CON (2.18 y 0.11 g/min; p=0.076). No differences in heart rate or ratings of perceived exercise were found during post-rehydration exercise. These results suggest that ingesting chicken noodle soup after exercise in the heat improves subsequent endurance exercise capacity, possibly through enhanced CHO oxidation

THE DISTRIBUTION SYSTEM OF PUBLIC HOUSING BASED ON MULTI-OBJECTIVE MATCHING: A CASE STUDY OF HUANGSHI CITY

The rapid rate of China’s urbanization in recent years arises greater demand of houses. To ease the housing shortage, Chinese authority has been building or collecting a large amount of public housing. However, as the large-scale construction of public housing has been promoted, an increasing number of people focus their eyes on the equitable distribution of these houses. This paper aims to establish a distribution system of public housing with the research in Huangshi City (a city in central China). We affirm the importance of priority and housing preference of applicant families, on the basis of which we discuss operating principle of the distribution system based on the multi-objective programming, and advance two ways of model solutions as well. At last, we propose an algorithm instance to verify feasibility of the distribution system, and make the comparison between two types of algorithms as well

A Case Study of Alpine Lakes in the Mount Everest Region

Abstract This study presents satellite data and in situ measurements to estimate the concentration of suspended solids in high-altitude and remote lakes of the Himalayas. Suspended particulate matter (SPM) concentrations measured in 13 lakes to the south of Mount Everest (Nepal) in October 2008 and reflectance values of the Advanced Visible and Near Infrared Radiometer type 2 (AVNIR-2) onboard ALOS, acquired a few days after the fieldwork activities concluded, were combined to build a relationship (R2  =  0.921) for mapping SPM concentrations in lakes of the Mount Everest region. The satellite-derived SPM concentrations were compared with in situ data (R2  =  0.924) collected in the same period in 4 additional lakes, located to the north of Mount Everest (Tibet, China). The 13 water samples collected in lakes in Nepal were also used to investigate the absorption coefficients of particles ap(λ) and colored, dissolved organic matter aCDOM(λ), with the aim of parameterizing a bio-optical model. An accurate m..

Effect of Exercise Training on Microvascular Function in African American and Caucasian Women

African Americans (AA), especially women, exhibit long-standing disparities in cardiovascular disease (CVD) and obesity. The prevalence of endothelial dysfunction, directly linked to hypertension, is considerably greater in AA than Caucasians (C). Vascular smooth muscle function (mediating endothelium-independent vasodilation) is also related to CVD risk factors but is underappreciated because most literature in C suggest endothelium-independent vasodilatory response is resistance to change with disease (hypertension) or exercise training. Furthermore, the regulation of local skeletal muscle blood flow (an important site of peripheral resistance) has not been sufficiently assessed. Microdialysis is the only method that allows monitoring of microvascular blood flow while affecting the local tissue with pharmacological agents in the absence of systemic, or organ level, effects in humans. Microvascular blood flow was assessed by microdialysis in vivo in skeletal muscle before and after 12 weeks of aerobic exercise training in young, obese AA and C women. Our preliminary data suggested that microvascular endothelial function, assessed by percent change in blood flow from basal (Δ Blood Flow) in response to acetylcholine perfusion was improved in both obese AA (n=5) and obese C (n=4) women. Microvascular endothelium-independent blood flow, assessed by percent change in blood flow from baseline (Δ Blood Flow) upon addition of sodium nitroprusside to the perfusate, was improved in AA (n=3) but not in C (n=9) women. Exercise training may improve endothelium-dependent vascular function in both AA and C, but improve endothelium-independent vascular function only in AA. Results of this study have potential to inform preventive interventions including lifestyle and pharmacological approaches designed to reduce disparities in hypertension and end-organ damage

Induction of WNT16 via peptide-mRNA nanoparticle-based delivery maintains cartilage homeostasis

Osteoarthritis (OA) is a progressive joint disease that causes significant disability and pain and for which there are limited treatment options. We posit that delivery of anabolic factors that protect and maintain cartilage homeostasis will halt or retard OA progression. We employ a peptide-based nanoplatform to deliver Wingless and the name Int-1 (WNT) 16 messenger RNA (mRNA) to human cartilage explants. The peptide forms a self-assembled nanocomplex of approximately 65 nm in size when incubated with WNT16 mRNA. The complex is further stabilized with hyaluronic acid (HA) for enhanced cellular uptake. Delivery of peptide-WNT16 mRNA nanocomplex to human cartilage explants antagonizes canonical β-catenin/WNT3a signaling, leading to increased lubricin production and decreased chondrocyte apoptosis. This is a proof-of-concept study showing that mRNA can be efficiently delivered to articular cartilage, an avascular tissue that is poorly accessible even when drugs are intra-articularly (IA) administered. The ability to accommodate a wide range of oligonucleotides suggests that this platform may find use in a broad range of clinical applications

Suppression of experimental arthritis through AMP-activated protein kinase activation and autophagy modulation

Autophagy plays a central role in various disease processes. However, its contribution to inflammatory arthritides such as rheumatoid arthritis (RA) is unclear. We observed that autophagy is engaged in the K/BxN serum transfer model of RA but autophagic flux is severely impaired. Metformin is an anti-diabetic drug that has been shown to stimulate autophagy. Induction of autophagic flux, through metformin-mediated AMP-activated protein kinase (AMPK) activation and interruption of mammalian target of rapamycin (mTOR) signaling mitigated the inflammation in experimental arthritis. Further investigation into the effects of metformin suggest that the drug directly activates AMPK and dose-dependently suppressed the release of TNF-α, IL-6, and MCP-1 by macrophages while enhancing the release of IL-10 in vitro. In vivo, metformin treatment significantly suppressed clinical arthritis and inflammatory cytokine production. Mechanistic studies suggest that metformin exerts its anti-inflammatory effects by correcting the impaired autophagic flux observed in the K/BxN arthritis model and suppressing NF-κB-mediated signaling through selective degradation of IκB kinase (IKK). These findings establish a central role for autophagy in inflammatory arthritis and argue that autophagy modulators such as metformin may represent potential therapeutic agents for the treatment of RA

Non-thermal cytocidal effect of infrared irradiation on cultured cancer cells using specialized device

The definitive version is available at www.blackwell-synergy.comAs infrared penetrates the skin, thermal effects of infrared irradiation on cancer cells have been investigated in the field of hyperthermia. We evaluated non-thermal effects of infrared irradiation using a specialized device (1100-18000 nm with filtering of wavelengths between 1400 and 1500 nm and contact cooling) on cancer cells. In in vitro study, five kinds of cultured cancer cell lines (MCF7 breast cancer, HeLa uterine cervical cancer, NUGC-4 gastric cancer, B16F0 melanoma, and MDA-MB435 melanoma) were irradiated using the infrared device, and then the cell proliferation activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Proliferation of all the cancer cell lines was significantly suppressed by infrared irradiation. Total infrared output appeared to be correlated with cell survival. Increased temperature during infrared irradiation appeared not to play a role in cell survival. The maximum temperature elevation in the wells after each shot in the 20 and 40 J/cm2 culture was 3.8 degrees C and 6.9 degrees C, respectively. In addition, we have shown that infrared irradiation significantly inhibited the tumor growth of MCF7 breast cancer transplanted in severe combined immunodeficiency mice and MDA-MB435 melanoma transplanted in nude mice in vivo. Significant differences between control and irradiated groups were observed in tumor volume and frequencies of TUNEL-positive and Ki-67-positive cells. These results indicate that infrared, independent of thermal energy, can induce cell killing of cancer cells. As this infrared irradiation schedule reduces discomfort and side effects, reaches the deep subcutaneous tissues, and facilitates repeated irradiations, it may have potential as an application for treating various forms of cancer.ArticleCANCER SCIENCE. 101(6):1396-1402 (2010)journal articl