Additional file 1 of Effects of Enteromorpha prolifera polysaccharides on growth performance, intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress

Additional file 1: Table S1. The chemical and monosaccharide composition of seaweed-derived polysaccharides (SDP) from Enteromorpha prolifera. Table S2. The formulation and nutrient level of the basal diets. Table S3. Primer information of real-time quantitative PCR

Effects of element complexes containing Fe, Zn and Mn on artificial morel’s biological characteristics and soil bacterial community structures

<div><p>This study described the effects of elements (including Fe, Zn, Mn and their complexes) on the following factors in artificial morel cultivation: the characteristics of mycelia and sclerotia, soil bacterial community structures, yields and contents of microelements. The results indicated that the groups containing Mn significantly promoted mycelia growth rates, and all the experimental groups resulted in higher yields than the control (P<0.01), although their mycelia and sclerotia did not show obvious differences. It was also found that <i>Proteobacteria</i>, <i>Chloroflexi</i>, <i>Bacteroides</i>, <i>Firmicutes</i>, <i>Actinobacteria</i>, <i>Acidobacteria</i> and <i>Nitrospirae</i> were the dominated bacterial phyla. The Zn·Fe group had an unexpectedly high proportion (75.49%) of <i>Proteobacteria</i> during the primordial differentiation stage, while <i>Pseudomonas</i> also occupied a high proportion (5.52%) in this group. These results suggested that different trace elements clearly affected morel yields and soil bacterial community structures, particularly due to the high proportions of <i>Pseudomonas</i> during the primordial differentiation stage.</p></div

Protocols of rhuIL-2 treatment.

<p>Protocols of rhuIL-2 treatment.</p

Flow diagram of the study selection process.

<p>Flow diagram of the study selection process.</p