Table_6_Involvement of JMJ15 in the dynamic change of genome-wide H3K4me3 in response to salt stress.XLSX

Post-translational histone modifications play important roles in regulating chromatin structure and transcriptional regulation. Histone 3 lysine 4 trimethylation (H3K4me3) is a prominent histone modification mainly associated with gene activation. Here we showed that a histone demethylase, JMJ15, belonging to KDM5/JARID group, is involved in salt stress response in Arabidopsis thaliana. Jmj15 loss-of-function mutants displayed increased sensitivity to salt stress. Moreover, knockout of JMJ15 impaired the salt responsive gene expression program and affected H3K4me3 levels of many stress-related genes under salt-stressed condition. Importantly, we demonstrated that JMJ15 regulated the expression level of two WRKY transcription factors, WRKY46 and WRKY70, which were negatively involved in abiotic stress tolerance. Furthermore, JMJ15 directly bound to and demethylated H3K4me3 mark in the promoter and coding regions of WRKY46 and WRKY70, thereby repressing these two WRKY gene expression under salt stress. Overall, our study revealed a novel molecular function of the histone demethylase JMJ15 under salt stress in plants.</p

The Adsorption of Reactive Blue 19 Dye onto Cucurbit[8]uril and Cucurbit[6]uril: An Experimental and Theoretical Study

The adsorption behavior and mechanism of Reactive Blue 19 (RB19) on cucurbit[6]­uril (CB[6]) and cucurbit[8]­uril (CB[8]) were investigated. The adsorption isotherm data obtained at different temperatures were fitted well to the Langmuir isotherm, and according to this model, CB[8] and CB[6] exhibited maximum monolayer adsorption capacities of 714.29 and 100.5 mg/g, respectively, at 298.15 K. The adsorption thermodynamic functions Δ<i>G</i>, Δ<i>H</i>, and Δ<i>S</i> were evaluated and revealed that RB19 adsorption onto CB[8] and CB[6] is a spontaneous and enthalpy-driven process. The adsorption process was determined to follow pseudo-second-order kinetics, indicating that chemisorption dominates the adsorption process. Fourier tranform IR spectroscopy, thermogravimetric analysis, and density functional theory (DFT) calculations revealed that the formation of an inclusion complex is the main driving force of adsorption. The phenyl and sulfone moieties of RB19 reside inside the cavity of CB[8], but because of the small cavity, only the sulfone of RB19 resides inside the cavity of CB[6]. Time-dependent DFT calculations revealed that all of the absorption bands of RB19 derive from π → π* transitions, while for the adsorption product of CB[8], the bands located at 590 and 287 nm derive from π → π* transitions and the bands located at 254 and 202 nm mainly derive from intermolecular charge transfer (ICT)

Prevalence of Type 2 Diabetes among High-Risk Adults in Shanghai from 2002 to 2012

<div><p>Objective</p><p>The objective of this study was to evaluate the trend and prevalence of prediabetes and diabetes among high-risk adults in Shanghai from 2002 to 2012.</p><p>Methods</p><p>From 2002 to 2012, 10043 subjects with known risk factors for diabetes participated in the diabetes-screening project at the Shanghai Sixth People’s Hospital of Shanghai Jiao Tong University. All participants were asked to complete a nurse-administered standard questionnaire concerning age, sex, smoking status, and personal and family histories of diabetes, cardiovascular disease, stroke, hypertension and other diseases. The participants’ body mass index scores, blood pressures and blood glucose levels at 0, 30, 60, 120 and 180 min were measured in response to a 75 g oral glucose tolerance test.</p><p>Results</p><p>The overall prevalence of diabetes increased from 27.93% to 34.78% between 2002 and 2012 in high-risk subjects. The study also showed that the prevalence increased much faster in male compared to female subjects. Specifically, an increased rate was seen in middle-aged men, with no change observed in middle-aged females over the eleven-year period.</p><p>Conclusion</p><p>This study showed that sex, age, parental diabetic history, and being overweight were associated with an increased risk for diabetes in high-risk people. Therefore, as prediabetes and diabetes are highly prevalent in people with multiple diabetes risk factors in Shanghai, screening programs targeting these individuals may be beneficial.</p></div

Scatter plots of BMI versus Age.

<p>Scatter plots of BMI versus Age.</p

Overall prevalence of diabetes from 2002 to 2012.

<p>Abbreviations: BMI, body mass index; SE, standard error of the mean.</p

Characteristics of female high-risk Chinese adults from 2002 to 2012.

<p>Abbreviations: BMI, body mass index; OGTT, oral glucose tolerance test.</p

Characteristics of all high-risk Chinese adults from 2002 to 2012.

<p>Abbreviations: BMI, body mass index; OGTT, oral glucose tolerance test.</p