2 research outputs found
TRIB2-mediated modulation of AMPK promotes hepatic insulin resistance
Insulin resistance and its linked health complications are increasing in prevalence. Recent work has caused the role of Tribbles2 (TRIB2) in metabolism and cellular signaling to be increasingly appreciated, but its role in the progression of insulin resistance has not been elucidated. Here, we explore the functions of TRIB2 in modulating insulin resistance and the mechanism involved in insulin resistance mice and palmitic acid (PA) treated HepG2 cells. We demonstrate that whole-body knockout and hepatic-specific TRIB2 deficiency protect against diet-induced insulin resistance, inflammation and ER stress. Accordingly, upregulation of TRIB2 in the liver aggravates these metabolic disturbances in HFD-induced mice and ob/ob mice. Mechanistically, TRIB2 directly binds to the αγ-SBS domain of PRKAB through its pseudokinase domain, subsequently inhibiting the formation and activity of the AMPK complex. Moreover, the results of intervention against AMPK suggest that the effects of TRIB2 depend on AMPK. Our findings reveal that TRIB2 is a novel target for the treatment of insulin resistance and its associated metabolic complications and clarify the function of TRIB2 as a regulatory component of AMPK activity.</p
Defect-Related Luminescent Mesoporous Silica Nanoparticles Employed for Novel Detectable Nanocarrier
Uniform and well-dispersed walnut
kernel-like mesoporous silica
nanoparticles (MSNs) with diameters about 100 nm have been synthesized
by a templating sol–gel route. After an annealing process,
the as-obtained sample (DLMSNs) inherits the well-defined morphology
and good dispersion of MSNs, and exhibits bright white-blue luminescence,
higher specific surface area and pore volume, and better biocompatibility.
The drug loading and release profiles show that DLMSNs have high drug
loading capacity, and exhibit an initial burst release followed by
a slow sustained release process. Interestingly, the luminescence
intensity of the DLMSNs-DOX system increases gradually with the increase
of cumulative released DOX, which can be verified by the confocal
laser scanning images. The drug carrier DLMSNs can potentially be
applied as a luminescent probe for monitoring the drug release process.
Moreover, the DLMSNs-DOX system exhibits potent anticancer effect
against three kinds of cancer cells (HeLa, MCF-7, and A549 cells)