Numerical analysis of aeroelastic characteristics of airship envelope

Stratospheric airship has great advantages, such as long-endurance, large coverage area, low-cost and so on, these advantages make airship be an ideal stratospheric platform and become highly valued. Airship envelope is a large inflatable membrane structure. As a key component, the envelope features flexible and large displacement. There is strong coupling between envelope structure and the ambient air when airship operating in the high altitude sky. The coupling characteristics have great impact on the aerodynamic and structural performance of airship. Aiming at the aeroelastic characteristics of the envelope structure, a fluid-structure coupled computational method is presented basing on a finite element program. As an example, the envelope structure of airship is computed and the S-A turbulent model is used. The envelope drag coefficient under different attack angle is computed. The contrast between experimental results coming from reference paper and numerical results highlight the correctness of this method. With the developed computational approach, the NPL envelope is also analyzed. The changes of length to diameter ratio, max cross section location and Reynolds number are studied and the aeroelastic characteristics of flexible envelope are analyzed. These results can give some valuable information for precise forecast of the overall airship performance

Dynamics testing and simulation of inflatable deployable

The inflatable deployablemembrane antenna structures have many advantages such as small folding size, high reliability and low cost. The structure mainly consists of its center hub, thin-plate ribs, inflatable thermo-curing torus, reflected membrane and inflation control system. This paper establishes a deployable system to simulate zero-gravity based on the parabolic membrane antenna with inflatable torus and tests the deployable process. The shell-membranes finite element model of the antenna structuresis modeled to simulateof the dynamics charactersof the structure. After that the effectsof the different inflatable pressure inside its support torus, the temperature of thermos-curing on the dynamic characteristics are also discussed.Finally,the dynamic charactersof the inflatable antenna was tested on the condition of the horizontal suspension system with 12 elastic strings and the fully structural vibrational frequency were given, and the mode of vibration and damping ratio was verified to the correctness of the simulation method. These results provide the reference for the design of inflatable deployment antenna structures

Numerical simulations for gas-structure interaction in inflated deployment of folded membrane boom

AbstractIt is very important for gas-structure interaction between compressible ideal gas and elastic structure of space folded membrane booms during the inflatable deployment. In order to study this gas-structure interaction problem, Arbitrary Lagrangian-Eulerian (ALE) finite element method was employed. Gas-structure interaction equation was built based on equilibrium integration relationship, and solved by operator split method. In addition, numerical analysis of V-shape folded membrane booms inflated by gas was given, the variation of inner pressure as well as deployment velocities of inflatable boom at different stage were simulated. Moreover, these results are consistent with the experiment of the same boom, which shows that both ALE method and operator split method are feasible and reliable methods to study gas-structure interaction problem

Serum 25(OH)D levels are associated with disease activity and renal involvement in initial-onset childhood systemic lupus erythematosus

BackgroundVitamin D deficiency is common in patients with systemic lupus erythematosus (SLE) and may affect their disease activity and severity.ObjectiveThis study aims to assess the vitamin D status in patients with initial-onset SLE during childhood and its association with the clinical and laboratory markers of disease activity.MethodThis is a retrospective study that includes 168 patients with initial-onset SLE during childhood and 109 healthy children as controls. Clinical and laboratory data were recorded. The area under the curve (AUC) method was used to evaluate the efficacy of double-stranded deoxyribonucleic acid (dsDNA), lower 25(OH)D and complement 3 (C3) alone and in combination to diagnose the presence of renal damage in children with SLE.ResultCompared with the controls (25.53 ± 7.02 ng/ml), patients with initial-onset SLE during childhood have lower serum 25(OH)D levels (18.63 ± 5.32 ng/ml) (P < 0.05). Among patients with initial-onset SLE during childhood, SLEDAI-2K scores are significantly higher in the vitamin D insufficiency (median = 14.5) and vitamin D deficiency (median = 14.0) groups than in the vitamin D sufficiency group (median = 9.0) (P < 0.05). Patients with initial-onset SLE during childhood with lower 25(OH)D levels are more likely to have lupus nephritis (LN) and a higher SDI score (P < 0.05). Compared with patients with other types of LN (16.69 ± 3.90 ng/ml), patients with type V LN have lower levels of 25(OH)D (12.27 ± 3.53 ng/ml) (P < 0.05). The AUC was 0.803 when dsDNA antibody, 25(OH)D level and C3 were used in combination to diagnose LN in patients with SLE.ConclusionVitamin D deficiency and insufficiency are closely related to an increase in SLEDAI and SDI scores. Significant decrease in vitamin D level is a risk factor for LN

Identification and characterization of a potential strain for the production of polyhydroxyalkanoate from glycerol

While poly (3-hydroxybutyrate) (PHB) holds promise as a bioplastic, its commercial utilization has been hampered by the high cost of raw materials. However, glycerol emerges as a viable feedstock for PHB production, offering a sustainable production approach and substantial cost reduction potential. Glycerol stands out as a promising feedstock for PHB production, offering a pathway toward sustainable manufacturing and considerable cost savings. The identification and characterization of strains capable of converting glycerol into PHB represent a pivotal strategy in advancing PHB production research. In this study, we isolated a strain, Ralstonia sp. RRA (RRA). The strain exhibits remarkable proficiency in synthesizing PHB from glycerol. With glycerol as the carbon source, RRA achieved a specific growth rate of 0.19 h−1, attaining a PHB content of approximately 50% within 30 h. Through third-generation genome and transcriptome sequencing, we elucidated the genome composition and identified a total of eight genes (glpR, glpD, glpS, glpT, glpP, glpQ, glpV, and glpK) involved in the glycerol metabolism pathway. Leveraging these findings, the strain RRA demonstrates significant promise in producing PHB from low-cost renewable carbon sources

GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline

Nuage are amorphous ultrastructural granules in the cytoplasm of male germ cells as divergent as Drosophila, Xenopus, and Homo sapiens. Most nuage are cytoplasmic ribonucleoprotein structures implicated in diverse RNA metabolism including the regulation of PIWI-interacting RNA (piRNA) synthesis by the PIWI family (i.e., MILI, MIWI2, and MIWI). MILI is prominent in embryonic and early post-natal germ cells in nuage also called germinal granules that are often associated with mitochondria and called intermitochondrial cement. We find that GASZ (Germ cell protein with Ankyrin repeats, Sterile alpha motif, and leucine Zipper) co-localizes with MILI in intermitochondrial cement. Knockout of Gasz in mice results in a dramatic downregulation of MILI, and phenocopies the zygotene–pachytene spermatocyte block and male sterility defect observed in MILI null mice. In Gasz null testes, we observe increased hypomethylation and expression of retrotransposons similar to MILI null testes. We also find global shifts in the small RNAome, including down-regulation of repeat-associated, known, and novel piRNAs. These studies provide the first evidence for an essential structural role for GASZ in male fertility and epigenetic and post-transcriptional silencing of retrotransposons by stabilizing MILI in nuage

Discovery of an orally active benzoxaborole prodrug effective in the treatment of Chagas disease in non-human primates

Trypanosoma cruzi, the agent of Chagas disease, probably infects tens of millions of people, primarily in Latin America, causing morbidity and mortality. The options for treatment and prevention of Chagas disease are limited and underutilized. Here we describe the discovery of a series of benzoxaborole compounds with nanomolar activity against extra- and intracellular stages of T. cruzi. Leveraging both ongoing drug discovery efforts in related kinetoplastids, and the exceptional models for rapid drug screening and optimization in T. cruzi, we have identified the prodrug AN15368 that is activated by parasite carboxypeptidases to yield a compound that targets the messenger RNA processing pathway in T. cruzi. AN15368 was found to be active in vitro and in vivo against a range of genetically distinct T. cruzi lineages and was uniformly curative in non-human primates (NHPs) with long-term naturally acquired infections. Treatment in NHPs also revealed no detectable acute toxicity or long-term health or reproductive impact. Thus, AN15368 is an extensively validated and apparently safe, clinically ready candidate with promising potential for prevention and treatment of Chagas disease

Antagonistic Changes in Sensitivity to Antifungal Drugs by Mutations of an Important ABC Transporter Gene in a Fungal Pathogen

Fungal pathogens can be lethal, especially among immunocompromised populations, such as patients with AIDS and recipients of tissue transplantation or chemotherapy. Prolonged usage of antifungal reagents can lead to drug resistance and treatment failure. Understanding mechanisms that underlie drug resistance by pathogenic microorganisms is thus vital for dealing with this emerging issue. In this study, we show that dramatic sequence changes in PDR5, an ABC (ATP-binding cassette) efflux transporter protein gene in an opportunistic fungal pathogen, caused the organism to become hypersensitive to azole, a widely used antifungal drug. Surprisingly, the same mutations conferred growth advantages to the organism on polyenes, which are also commonly used antimycotics. Our results indicate that Pdr5p might be important for ergosterol homeostasis. The observed remarkable sequence divergence in the PDR5 gene in yeast strain YJM789 may represent an interesting case of adaptive loss of gene function with significant clinical implications