Characteristics of D39 <i>S</i>. <i>pneumoniae</i> mutants used across experiments.

<p>Characteristics of D39 <i>S</i>. <i>pneumoniae</i> mutants used across experiments.</p

Fold change in CFU/mL from baseline in saline (n = 3), cell-culture medium (DMEM) (n = 3), THB supplemented with fetal calf serum (n = 3) and pleural fluid (n = 6).

<p>Each graph represents a different S. <i>pneumoniae</i> clinical isolate (a) serotype 8 (blood) (b) serotype 19A (blood) and (c) serotype 19A (pleural fluid). Bacterial CFU/mL in each fluid was determined at 4, 8, 12, 18, 24, 28 and 48 hours. Fluid characteristics are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188833#pone.0188833.t003" target="_blank">Table 3</a>.</p

Correlation of <i>S</i>. <i>pneumoniae</i> (n = 25) growth in pleural fluid with and without cells (n = 11 pairs) expressed as the fold change from baseline inoculum at 24 hours (n = 275 pairs), p<0.001.

<p>Correlation of <i>S</i>. <i>pneumoniae</i> (n = 25) growth in pleural fluid with and without cells (n = 11 pairs) expressed as the fold change from baseline inoculum at 24 hours (n = 275 pairs), p<0.001.</p

Pleural fluid characteristics for individual experiments.

<p>Pleural fluid characteristics for individual experiments.</p

The median growth of <i>S</i>. <i>pneumoniae</i> (n = 25) in human pleural fluid (n = 11) was consistent across all (a) pleural fluid samples (b) pneumococcal isolates and (c) serotypes.

<p>Each dot-point represents data analyzed as (a) the median growth of all <i>S</i>. <i>pneumoniae</i> in each pleural fluid samples (n = 11), (b) individual <i>S</i>. <i>pneumoniae</i> isolates (n = 25) and the median growth of each isolate across pleural fluid samples and (c) <i>S</i>. <i>pneumoniae</i> grouped according to serotype (n = 13); data from the pleural fluid samples (n = 11) is pooled when more than one serotype is available and includes serotypes 1 (n = 2), 6B, 6C, 8 (n = 3), 10A, 11A (n = 2), 12F, 19A (n = 7), 19F, 21, 22F (n = 2), 35B and 3 reference strain. Proliferation was significant at 24hrs across all pneumococci, serotypes and pleural fluids, p<0.001. The box plot represents the median and IQR of the dot plot data; whiskers represent the 95^th percentile. Pleural fluid characteristics are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188833#pone.0188833.t003" target="_blank">Table 3</a>.</p

List of <i>Streptococcus pneumoniae</i> serotypes and other bacteria used in this study.

<p>List of <i>Streptococcus pneumoniae</i> serotypes and other bacteria used in this study.</p

D39 <i>S</i>. <i>pneumoniae</i> wild type and Δ<i>psaA</i> growth in pleural fluid (n = 16) at 24 hrs with and without manganese (Mn²⁺) 3μM supplementation.

<p>Δ<i>psaA</i> growth was restored with manganese supplementation, p<0.001.</p

The fold change in CFU/mL of other bacterial pathogens (n = 14) including <i>P</i>. <i>aeruginosa (</i>△), <i>S</i>. <i>aureus</i> (О), S. <i>anginosis</i> group (×), <i>E</i>. <i>coli</i> (□), <i>Klebsiella sp</i>.(▽), <i>E</i>. <i>faecalis</i> (⊕), <i>M</i>. <i>catarrhalis</i> (✯).

<p>Each dot point represents the median fold change of each bacterial strain across pleural fluid (n = 7) samples at 24hrs. Data from other bacterial pathogens is compared to previous results of <i>S</i>. <i>pneumoniae</i> (n = 25) growth in pleural fluid (n = 11), p<0.001. The box plot represents the median and IQR; whiskers represent the 95^th percentile.</p