Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals

In the present study genipin crosslinked chitosan (CHI) hydrogels, which had been constructed and reported in our previous studies (Lei Gao, et al. Colloids Surf. B Biointerfaces. 2014, 117: 398), were further evaluated for their advantage as a carrier for silver sulfadiazine (AgSD) nanocrystal systems. Firstly, AgSD nanocrystals with a mean particle size of 289 nm were prepared by wet milling method and encapsulated into genipin crosslinked CHI hydrogels. AgSD nanocrystals displayed a uniform distribution and very good physical stability in the hydrogel network. Swelling-dependent release pattern was found for AgSD nanocrystals from hydrogels and the release profile could be well fitted with Peppas equation. When AgSD nanocrystals were encapsulated in hydrogels their fibroblast cytotoxicity decreased markedly, and their antibacterial effects against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were still comparable to unencapsulated AgSD nanocrystals. In vivo evaluation in excision and burn cutaneous wound models in mice showed that AgSD nanocrystal hydrogels markedly decreased the expression of inflammatory cytokine IL-6, but increased the levels of growth factors VEGF-A and TGF-β1. Histopathologically, the wounds treated by hydrogels containing AgSD nanocrystals showed the best healing state compared with commercial AgSD cream, hydrogels containing AgSD bulk powders and blank hydrogels. The wounds treated by AgSD nanocrystal hydrogels were dominated by marked fibroblast proliferation, new blood vessels and thick regenerated epithelial layer. Sirius Red staining assay indicated that AgSD nanocrystal hydrogels resulted in more collagen deposition characterized by a large proportion of type I fibers. Our study suggested that genipin-crosslinked CHI hydrogel was a potential carrier for local antibacterial nanomedicines

sj-tif-3-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-tif-3-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-docx-6-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-docx-6-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-tif-1-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-tif-1-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-docx-7-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-docx-7-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-docx-5-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-docx-5-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-docx-8-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-docx-8-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-tif-2-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-tif-2-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

sj-tif-4-scm-10.1177_00369330231187655 - Supplemental material for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis

Supplemental material, sj-tif-4-scm-10.1177_00369330231187655 for The impact of sarcopenia on prognosis of patients with pancreatic cancer: A systematic review and meta-analysis by Hui Gan, Jiarong Lan, Hongxia Bei and Guangxing Xu in Scottish Medical Journal</p

Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

Chirality is one of the significant biochemical signatures of life. Nearly all biological polymers are homochiral as they usually show high preference toward one specific enantiomer. This phenomenon inspires us to design biomaterials with chiral units and study their interactions with cells and other biological entities. In this article, through adopting three pairs of aliphatic amino acids with different hydrophobic side groups as chiral species, and using two adhesive cell lines as examples, we show that the chirality of polymer brushes can trigger differential cell behaviors on the enantiomorphous surfaces, and more interestingly, such chiral effect on cellular behaviors can be modulated in a certain extent by varying the hydrophobic side groups of the chiral moieties composing the polymers. This work not only proves the versatility of the chiral effect at the cell level but also demonstrates a method to bridge the gap between organic signal molecules and biomaterials. It thus points out a promising approach for designing novel biomaterials based on the chiral effect, which will be an important complement for conventional strategies in the study of biomaterials