3 research outputs found
<sup>99m</sup>Tc-MAG3-Aptamer for Imaging Human Tumors Associated with High Level of Matrix Metalloprotease‑9
The human matrix metalloprotease 9 (hMMP-9) is involved
in many
physiological processes such as tissue remodeling. Its overexpression
in tumors promotes the release of cancer cells thus contributing to
tumor metastasis. It is a relevant marker of malignant tumors. We
selected an RNA aptamer containing 2′-fluoro, pyrimidine ribonucleosides,
that exhibits a strong affinity for hMMP-9 (<i>K</i><sub>d</sub> = 20 nM) and that discriminates other human MMPs: no binding
was detected to either hMMP-2 or -7. Investigating the binding properties
of different MMP-9 aptamer variants by surface plasmon resonance allowed
the determination of recognition elements. As a result, a truncated
aptamer, 36 nucleotides long, was made fully resistant to nuclease
following the substitution of every purine ribonucleoside residue
by 2′-<i>O</i>-methyl analogues and was conjugated
to <i>S</i>-acetylmercaptoacetyltriglycine for imaging purposes.
The resulting modified aptamer retained the binding properties of
the originally selected sequence. Following <sup>99m</sup>Tc labeling,
this aptamer was used for ex vivo imaging slices of human brain tumors.
We were able to specifically detect the presence of hMMP-9 in such
tissues
Additional file 1: of Increasing incidence of central nervous system (CNS) tumors (2000–2012): findings from a population based registry in Gironde (France)
Table S1. Distribution and standardized incidence rates (reference populations Europe, US, world) by age, sex and histological type, Gironde CNS registry, 2000–2012. (DOCX 18 kb
Additional file 1: Table S1. of Characteristics of gliomas in patients with somatic IDH mosaicism
The characteristics of those of the 32 previously published patients. (XLSX 15 kb