Pisces IV submersible observations in the epicentral region of the 1929 Grand Banks earthquake

The PISCES IVsubmersible was used to investigate the upper continental slope around 44 ON, 56 W, near the epicentre of the 1929 Grand Banks earthquake. Four dives in water depths of 800-2000 m were undertaken to observe speci3c features identijied with the SeaMARC I sidescan system in 1983. Two dives were made in the head of Eastern Valley where pebbly mudstones ofprobable Pleistocene age were recognized outcropping on the seafloor. Constructional features of cobbles and boulders, derived by exhumation and reworking of the pebbly mudstone, were also observed. These include gravel/sand bedforms (transverse waves) on the valley floor. Slope failure features in semiconsolidated mudstone were recognized on two dives onto the St. Pierre slope. Exposures in these mudstones are rapidly eroded by intense burrowing by benthic organisms

Sedimentary and structural evolution of a relict subglacial to subaerial drainage system and its hydrogeological implications: an example from Anglesey, north Wales, UK

Subglacial drainage systems exert a major control on basal-sliding rates and glacier dynamics. However, comparatively few studies have examined the sedimentary record of subglacial drainage. This is due to the paucity of modern analogues, the limited recognition and preservation of upper flow regime deposits within the geological record, and the difficulty of distinguishing subglacial meltwater deposits from other meltwater sediments (e.g. glacier outburst flood deposits). Within this study, the sedimentological and structural evolution of a subglacial to subaerial (ice-marginal/proglacial) drainage system is examined. Particular emphasis is placed upon the genetic development and preservation of upper flow regime bedforms and specifically recognising them within a subglacial meltwater context. Facies are attributed to subglacial meltwater activity and record sedimentation within a confined, but progressively enlargening, subglacial channel system produced under dune to upper flow regime conditions. Bedforms include rare large-scale sinusoidal bedding with syn-depositional deformation produced by current-induced traction and shearing within the channel margins. Subglacial sedimentation culminated with the abrupt change to a more ephemeral drainage regime indicating channel-abandonment or a seasonal drainage regime. Retreat of the ice margin, led to the establishment of subaerial drainage with phases of sheet-flow punctuated by channel incision and anastomosing channel development under diurnal, ablation-related, seasonal discharge. The presence of extensive hydrofracture networks demonstrate that proglacial groundwater-levels fluctuated markedly and this may have influenced later overriding of the site by an ice stream

Observations and Empirical Scalings of the High-Confinement Mode Pedestal on Alcator C-Mod

and disposal, in whole or in part, by or for the United States government is permitted. Observations and empirical scalings of the high-confinement mode pedestal on Alcator C-Mod J.W. Hughes, D.A. Mossessian, A.E. Hubbard, B. LaBombard, E.S. Marmar On the Alcator C-Mod tokamak [Phys. Plasmas 1, 1511, (1994)], radial pro-files of electron temperature (Te) and density (ne) are measured at the plasma edge with millimeter resolution Thomson scattering [Rev. Sci. Instrum. 72, 1107 (2001)]. Edge transport barriers in the high confinement regime (H-mode) exhibit Te, ne pedestals with typical widths of 2–6 mm, with the Te pedestal on average slightly wider than and inside the ne pedestal. Measure-ments at both the top and the base of the pedestal are consistent with profiles obtained using other diagnostics. The two primary H-mode regimes on C-Mod, enhanced Dα (EDA) and edge-localized mode free (ELM-free), have been ex-amined for differences in pedestals. EDA operation is favored by high edge collisionality ν, in addition to high edge safety factor q95. Scaling studies at fixed shape yield little systematic variation of pedestal widths with plasma parameters, though higher triangularity is seen to increase the ne pedestal width dramatically. Pedestal heights and gradients show the clearest depen-dencies on plasma control parameters. Pedestal ne and Te both scale linearly with plasma current IP, while pedestal Te depends strongly on power flowing from the core plasma into the scrape-off layer PSOL. The electron pressure (pe) pedestal and pe gradient both scale with I 2 P

Identification of reference miRNAs in plasma useful for the study of oestrogen-responsive miRNAs associated with acquired Protein S deficiency in pregnancy

Background Accumulating evidence indicate that circulating microRNAs (miRNAs) are useful independent non-invasive biomarkers, with unique miRNA signatures defined for various pathophysiological conditions. However, there are no established universal housekeeping miRNAs for the normalisation of miRNAs in body fluids. We have previously identified an oestrogen-responsive miRNA, miR-494, in regulating the anticoagulant, Protein S, in HuH-7 liver cells. Moreover, increased thrombotic risk associated with elevated circulating oestrogen levels is frequently observed in pregnant women and oral contraceptive users. In order to identify other oestrogen-responsive miRNAs, including miR-494, that may be indicative of increased thrombotic risk in plasma, we used nanoString analysis to identify robust and stable endogenous reference miRNAs for the study of oestrogen-responsive miRNAs in plasma. Results We compared the plasma miRNA expression profile of individuals with: (1) Low circulating oestrogens (healthy men and non-pregnant women not taking oral contraceptives), (2) High circulating synthetic oestrogens, (women taking oral contraceptives) and (3) High circulating natural oestrogens (pregnant females >14 weeks gestation). From the nanoString analyses, 11 candidate reference miRNAs which exhibited high counts and not significantly differentially expressed between groups were selected for validation using realtime quantitative polymerase chain reaction (RT-qPCR) and digital droplet PCR (DDPCR) in pooled plasma samples, and the stability of their expression evaluated using NormFinder and BestKeeper algorithms. Four miRNAs (miR-25-5p, miR-188-5p, miR-222-3p and miR-520f) demonstrated detectable stable expression between groups and were further analysed by RT-qPCR in individual plasma samples, where miR-188-5p and miR-222-3p expression were identified as a stable pair of reference genes. The miRNA reference panel consisting of synthetic spike-ins cel-miR-39 and ath-miR159a, and reference miRNAs, miR-188-5p and miR-222-3p was useful in evaluating fold-change of the pregnancy-associated miRNA, miR-141-3p, between groups. Conclusion The miRNA reference panel will be useful for normalising qPCR data comparing miRNA expression between men and women, non-pregnant and pregnant females, and the potential effects of endogenous and synthetic oestrogens on plasma miRNA expression