Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites

The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug target sites. The analysis of conservation based on 4430 amino acid sequences identified high conservation in known functional regions as well as novel highly conserved sites. Highly variable clusters identified on the surface of NP may be associated with adaptation to different hosts and avoidance of the host immune defence. Ligand binding potential overlapping with high conservation was found in the tail-loop binding site and near the putative RNA binding region. The results provide the basis for developing antivirals that may be universally effective and have a reduced potential to induce resistance through mutations.Peer reviewe

On the Radio Polarization Signature of Efficient and Inefficient Particle Acceleration in Supernova Remnant SN 1006

We present a radio polarization study of SN 1006, based on combined VLA and ATCA observations at 20 cm that resulted in sensitive images with an angular resolution of 10 arcsec. The fractional polarization in the two bright radio and X-ray lobes of the SNR is measured to be 0.17, while in the southeastern sector, where the radio and non-thermal X-ray emission are much weaker, the polarization fraction reaches a value of 0.6 +- 0.2, close to the theoretical limit of 0.7. We interpret this result as evidence of a disordered, turbulent magnetic field in the lobes, where particle acceleration is believed to be efficient, and a highly ordered field in the southeast, where the acceleration efficiency has been shown to be very low. Utilizing the frequency coverage of our observations, an average rotation measure of ~12 rad/m2 is determined from the combined data set, which is then used to obtain the intrinsic direction of the magnetic field vectors. While the orientation of magnetic field vectors across the SNR shell appear radial, a large fraction of the magnetic vectors lie parallel to the Galactic Plane. Along the highly polarized southeastern rim, the field is aligned tangent to the shock, and therefore also nearly parallel to the Galactic Plane. These results strongly suggest that the ambient field surrounding SN 1006 is aligned with this direction (i.e., from northeast to southwest) and that the bright lobes are due to a polar cap geometry. Our study establishes that the most efficient particle acceleration and generation of magnetic turbulence in SN 1006 is attained for shocks in which the magnetic field direction and shock normal are quasi-parallel, while inefficient acceleration and little to no generation of magnetic turbulence obtains for the quasi-perpendicular case.Comment: Accepted for publication in Astronomical Journa

FINANCIAL PERFORMANCE IN MEAT AND POULTRY MANUFACTURING AND WHOLESALING: AN HISTORICAL PERSPECTIVE

The financial performance of meat and poultry manufacturing and wholesaling firms is examined for the period from 1970 to 1986. Measures of liquidity, solvency, profitability, cash generation, and efficiency reported in the Robert Morris Associates Annual Statement Studies are used to examine relative performance across the different industries. The results suggest a similar performance in the wholesaling and manufacturing industries across the period in terms of liquidity. Profitability levels are similar for meat and poultry firms, although the poultry firms show a higher level of variability across the period. It appears that poultry firms leveraged themselves relatively more than did meat firms during the period. In terms of cash generation and efficiency the meat manufacturing industry performs slightly better than the other industries.Agricultural Finance,

ESTABLISHING THE ECONOMIC IMPACT OF THE GREEN INDUSTRY ON LOUISIANA'S ECONOMY

Ornamental and horticultural products and services represent one of the growing sectors in agriculture. This study includes data collection and an input-out IMPLAN model used to estimate the total economic impact of the green industry on Louisianas economy. This model provided results in total impact in personal income, gross state product, employment, expenditures in selected sectors and multiplier tables.Production Economics,

Family-focused treatment for childhood depression: model and case illustrations

Although the evidence base for treatment of depressive disorders in adolescents has strengthened in recent years, less is known about the treatment of depression in middle to late childhood. A family-based treatment may be optimal in addressing the interpersonal problems and symptoms frequently evident among depressed children during this developmental phase, particularly given data indicating that attributes of the family environment predict recovery versus continuing depression among depressed children. Family-Focused Treatment for Childhood Depression (FFT-CD) is designed as a 15-session family treatment with both the youth and parents targeting two putative mechanisms involved in recovery: (a) enhancing family support, specifically decreasing criticism and increasing supportive interactions; and (b) strengthening specific cognitive-behavioral skills within a family context that have been central to CBT for depression, specifically behavioral activation, communication, and problem solving. This article describes in detail the FFT-CD protocol and illustrates its implementation with three depressed children and their families. Common themes/challenges in treatment included family stressors, comorbidity, parental mental health challenges, and inclusion/integration of siblings into sessions. These three children experienced positive changes from pre- to posttreatment on assessor-rated depressive symptoms, parent- and child-rated depressive symptoms, and parent-rated internalizing and externalizing symptoms. These changes were maintained at follow-up evaluations 4 and 9 months following treatment completion.K23 MH101238 - NIMH NIH HHS; R01 MH082856 - NIMH NIH HHS; R01 MH082861 - NIMH NIH HH