47 research outputs found
Aufbau einer niedersächsischen Forschungsdatenplattform für COVID-19-Studien: Von der Modellierung in openEHR über Datenimport bis zur Datenfilterung und -herausgabe
Das "Hip vacuum sign" - ein neues radiologisches Zeichen für ein femoroazetabuläres Impingement
Return to Sport von Freizeitsportlern nach arthroskopischer Korrektur eines femoroazetabulären Impingements
Prävalenz von Propionibacterium acnes im Glenohumeral- versus Subakromialraum bei Schulterarthroskopien mit intakter Rotatorenmanschette
LBS-Tenodese versus LBS-Tenotomie bei isolierten LBS-Läsionen - eine prospektiv randomisierte Studie mit Evaluation der Flexions- und Supinationskraft und objektiver Messung der Bizeps-Muskel-Distalisierung
Response of mature meniscal tissue to a single injurious compression and interleukin-1 in vitro
Objective:
To study mechanical overload of mature meniscal tissue under normal and pro-inflammatory conditions in vitro.
Method:
Three days after a single unconfined compression (strain: 25–75%, strain rate 1/s) of meniscal explants from 16 to 24 months-old cattle combined with interleukin-1-treatment (IL-1, 10 ng/ml) release of glycosaminoglycans (GAGs; dimethylmethylene blue (DMMB) assay), lactate dehydrogenase (LDH; cytotoxicity detection kit), and nitric oxide (NO; Griess assay), as well as gene transcription (quantitative reverse transcription polymerase chain reaction (RT-PCR)) and numbers of cells with condensed nuclei (CN; histomorphometry) were determined.
Results:
Mean peak stresses during compression were about five (25%), 11 (50%), and 30 MPa (75%), respectively. GAG and LDH release and numbers of CN increased whereas NO production and mRNA levels of matrix metalloproteinase (MMP)-2, -3 and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 decreased strain-dependently after compression. IL-1 induced an increase in GAG and NO release as well as MMP-2, -3 and ADAMTS-4 levels, but had no impact on the LDH release and slightly increased numbers of CN. However, in combination with compression the tissue responses were reduced and LDH and CN levels were increased compared to IL-1 alone.
Conclusion:
Our data suggest that a single impact compression induces cell damage and release of GAG and reduces the NO production and transcription of certain matrix-degrading enzymes. It also reduces the capacity of meniscal tissue to respond to IL-1, which might be related to the cell damage and suggests that the compression-related GAG release might rather be the result of immediate extracellular matrix-damage than a cell-mediated event. This, however, needs to be confirmed in future studies.ENDO-Stiftung (Hamburg, Germany)ENDO-Klinik (Hamburg, Germany