Management of phosphorus supply to Australian floricultural species

Young plants of Sticherus, waxflower (Chamelaucium) and two Caustis cultivars were grown in a soilless potting medium. The plants were fertilised at seven rates of the soluble monocalcium phosphate (MCP) fertiliser and one rate of the slightly soluble rock phosphate (RP). One group of plants was watered by a non-leaching and a second group of plants watered by a leaching method. Both Caustis cultivars (M63, B84) grown in the potting medium under the non-leaching regime did not show deficiency symptoms at no added P, but they showed P toxicity symptoms at MCP-P application rates > 11 g m-3. The critical P concentration in the shoots associated with a 10% decrease in yield from the maximum was 0.26% for Caustis M63 and 0.33% for Caustis B84. The two Caustis cultivars did not develop P toxicity symptoms or show any decline in shoot dry weight when fertilised with RP under both non-leaching and leaching regimes. Leaching reduced but did not eliminate P toxicity in both Caustis cultivars, although shoot dry weight did not differ significantly between the two watering regimes used. Waxflower grew poorly in the potting medium under the non-leaching regime at no added P or when fertilised with RP but did not develop P toxicity symptoms at any rate of P application as MCP, including the highest rate of 352 g m-3. The waxflower plants grew less well under the leaching than when grown under the non-leaching regime. The critical NaHCO3-extractable P level required for production of 90% relative dry weight of shoots of waxflower was about 46 mg kg-1 under the non-leaching and about 69 mg kg-1 medium under the leaching regime. Under the non-leaching regime the critical NaHCO3-extractable P level for toxicity of Caustis B84 was about 13 mg kg-1 while under the leaching regime this was higher at about 22 mg kg-1. Sticherus plants did not respond significantly to P application as either MCP or RP. However, Sticherus plants grew much better when grown under the leaching than when they were grown under the non-leaching regime. Although leaching conferred some advantages in the growth of Sticherus and in the reduction of P toxicity in Caustis, it also caused a considerable loss of P

Reduction in benefits of total flux expansion on divertor detachment due to parallel flows

The Super-X divertor (SXD) is an alternative divertor configuration leveraging total flux expansion at the outer strike point (OSP). Key features for the attractiveness of the SXD are facilitated detachment access and control, as predicted by the extended 2-point model (2PM). However, parallel flows are not consistently included in the 2PM. In this work, the 2PM is refined to overcome this limitation: the role of total flux expansion on the pressure balance is made explicit, by including the effect of parallel flows. In consequence, the effect of total flux expansion on detachment access and control is weakened, compared to predictions of the 2PM. This new model partially explains discrepancies between the 2PM and experiments performed on TCV, in ohmic L-mode scenarios, where in core density ramps in lower single-null (SN) configuration, the impact of the OSP major radius Rt on the CIII emission front movement in the divertor outer leg - used as a proxy for the plasma temperature - is substantially weaker than 2PM predictions; and in OSP sweeps in lower and upper SN configurations, with a constant core density, the peak parallel particle flux density at the OSP is almost independent of Rt, while the 2PM predicts a linear dependence. Finally, analytical and numerical modelling of parallel flows in the divertor is presented, to support the argument. It is shown that an increase in total flux expansion can favour supersonic flows at the OSP. Parallel flows are also shown to be relevant by analysing SOLPS-ITER simulations of TCV

Comparison of detachment in Ohmic plasmas with positive and negative triangularity

Detachment is investigated using core density ramps for lower single null Ohmic L-mode plasmas across a wide range of upper, lower, and total triangularity ($\delta$) in the TCV tokamak. It is universally found that detachment is more difficult to access with negative triangularity (NT) shaping. The outer divertor leg of discharges with $\delta\approx -0.3$ could not be cooled below 5 eV using core density ramps alone. The behavior of the upstream plasma and geometrical divertor effects (e.g. a reduced connection length at negative lower triangularity) do not fully explain the challenges of detaching NT plasmas. Langmuir probe measurements of the target heat flux widths ($\lambda_q$) remained constant within 30% across an upper triangularity scan, while the spreading factor $S$ was found to be lower by up to 50% in NT, indicating a generally lower integral SOL width. An interesting pattern has been observed in the particle balance where the line-averaged core density was typically higher in NT discharges for a given fuelling rate. Conversely, the divertor neutral pressure and integrated particle content were typically lower for the same line-averaged density. This indicates that NT plasmas may be closer to the sheath-limited regime than their PT counterparts, which could explain why NT is more challenging to detach

Discrimination of conventional and organic white cabbage from a long-term field trial study using untargeted LC-MS-based metabolomics

The influence of organic and conventional farming practices on the content of single nutrients in plants is disputed in the scientific literature. Here, large-scale untargeted LC-MS-based metabolomics was used to compare the composition of white cabbage from organic and conventional agriculture, measuring 1,600 compounds. Cabbage was sampled in 2 years from one conventional and two organic farming systems in a rigidly controlled long-term field trial in Denmark. Using Orthogonal Projection to Latent Structures-Discriminant Analysis (OPLS-DA), we found that the production system leaves a significant (p = 0.013) imprint in the white cabbage metabolome that is retained between production years. We externally validated this finding by predicting the production system of samples from one year using a classification model built on samples from the other year, with a correct classification in 83% of cases. Thus, it was concluded that the investigated conventional and organic management practices have a systematic impact on the metabolome of white cabbage. This emphasizes the potential of untargeted metabolomics for authenticity testing of organic plant products

GLP-1 Analogs Reduce Hepatocyte Steatosis and Improve Survival by Enhancing the Unfolded Protein Response and Promoting Macroautophagy

Nonalcoholic fatty liver disease (NAFLD) is a known outcome of hepatosteatosis. Free fatty acids (FFA) induce the unfolded protein response (UPR) or endoplasmic reticulum (ER) stress that may induce apoptosis. Recent data indicate ER stress to be a major player in the progression of fatty liver to more aggressive lesions. Autophagy on the other hand has been demonstrated to be protective against ER stress-induced cell death. We hypothesized that exendin-4 (GLP-1 analog) treatment of fat loaded hepatocytes can reduce steatosis by autophagy which leads to reduced ER stress-related hepatocyte apoptosis.Primary human hepatocytes were loaded with saturated, cis- and trans-unsaturated fatty acids (palmitic, oleic and elaidic acid respectively). Steatosis, induced with all three fatty acids, was significantly resolved after exendin-4 treatment. Exendin-4 sustained levels of GRP78 expression in fat-loaded cells when compared to untreated fat-loaded cells alone. In contrast, CHOP (C/EBP homologous protein); the penultimate protein that leads to ER stress-related cell death was significantly decreased by exendin-4 in hepatocytes loaded with fatty acids. Finally, exendin-4 in fat loaded hepatocytes clearly promoted gene products associated with macroautophagy as measured by enhanced production of both Beclin-1 and LC3B-II, markers for autophagy; and visualized by transmission electron microscopy (TEM). Similar observations were made in mouse liver lysates after mice were fed with high fat high fructose diet and treated with a long acting GLP-1 receptor agonist, liraglutide.GLP-1 proteins appear to protect hepatocytes from fatty acid-related death by prohibition of a dysfunctional ER stress response; and reduce fatty acid accumulation, by activation of both macro-and chaperone-mediated autophagy. These findings provide a novel role for GLP-1 proteins in halting the progression of more aggressive lesions from underlying steatosis in humans afflicted with NAFLD