Additional file 1 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 1: Figure S1. Mitochondrial genome sketch of A. amurensis (node ID marked in the figure). The orange color represents a major circular genome structure after resolving the duplicate region based on HiFi dat

Additional file 4 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 4: Table S5. Dispersed repeat sequences in the mitochondrial genome of A. amurensi

Additional file 5 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 5: Table S6. dN/dS ratios of each gene in the mitochondrial genome of Aquilegi

Additional file 6 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 6: Table S7. Nontandem repeat sequences in the mitochondrial genome of A. amurensi

Additional file 2 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 2: Figure S2. Phylogenetic relationships based on the mitochondrial genome of Aquilegia. The ML ultrafastbootstrap (ufbs) and BI posterior probability (PP) values are indicated above the branches. “*” are ufbs or PP of 10

Additional file 3 of Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications

Supplementary Material 3: Table S1. Information about the Aquilegia sequences data previously published. Table S2. Annotated genes list in the mitochondrial genome of A. amurensis. Table S3. SSRs in the mitochondrial genome of A. amurensis. Table S4. Tandem repeat sequences in the mitochondrial genome of A. amurensi

Examples of SSAP analysis on the control and treatment groups.

<p>Typical variations are marked by arrowheads. (Primer: BARE-1/H/M+TAG).</p

Sequence analysis of differentially methylated fragments isolated from MSAP in the stress groups of <i>Leymus chinensis</i>.

<p>Sequence analysis of differentially methylated fragments isolated from MSAP in the stress groups of <i>Leymus chinensis</i>.</p

Conization Using an Electrosurgical Knife for Cervical Intraepithelial Neoplasia and Microinvasive Carcinoma

<div><p>Objective</p><p>The aim of the present study was to evaluate the incidences of margin involvement, disease relapse, and complications in patients who had undergone conization using an electrosurgical knife (EKC) for cervical intraepithelial neoplasia (CIN) or microinvasive carcinomas (micro-CAs).</p><p>Materials and Methods</p><p>A retrospective case series analysis was performed with a total of 1359 patients who underwent EKC in Fudan University Shanghai Cancer Center between June 2004 and July 2010.</p><p>Results</p><p>The median age of the patients was 39 years old (range: 19-72). Conization revealed the presence of CIN in 1113 (81.9%) patients, micro-CA in 72 (5.3%) patients and invasive carcinomas in 44 (3.2%) patients. The remaining 130 (9.6%) patients were free of diseases in the cone specimens. Positive surgical margins, or endocervical curettages (ECCs) were found in 90 (7.6%) patients with CINs or micro-CAs. Three factors were associated with positive margins and ECCs and included age (>50 years; odds ratio (OR), 3.0, P<0.01), postmenopausal status (OR, 3.1, P<0.01) and microinvasive disease (OR, 2.7, P<0.01). One thousand and eighty-nine (92.0%) patients were followed-up regularly for a median follow-up duration of 46 months (range: 24-106 months). Disease relapse was documented in 50 (4.6%) patients. Eighty-two (6.0%) cases experienced surgical complications that needed to be addressed, including early or late hemorrhages, infections, cervical stenosis, etc.</p><p>Conclusions</p><p>Our patients demonstrated that EKC was an alternative technique for diagnosis and treatment of CIN or micro-CAs with relatively low rate of recurrence and acceptable rate of complications. A randomized clinical trial is warranted to compare EKC, CKC and LEEP in the management of CIN or micro-CA.</p></div

Tabulated changes of MSAP (A) and SSAP (B) profiles showing the four patterns of cytosine methylation alterations, CG hypo, CHG hypo, CG hyper and CHG hyper, in the warming, N addition and warming+N addition compared with the CK.

<p>Tabulated changes of MSAP (A) and SSAP (B) profiles showing the four patterns of cytosine methylation alterations, CG hypo, CHG hypo, CG hyper and CHG hyper, in the warming, N addition and warming+N addition compared with the CK.</p