Evaluación de la actividad antibacteriana in vitro de los extractos de Caesalpinia spinosa “tara” y Eucalyptus sp. “eucalipto”

The antibacterian activity of extract of Caesalpinia spinosa and Eucalyptus sp. "eucalipto" was valued using bacterials capes gram positives (Staphylococcus aureus and Bacyllus subtilis) and gram negatives (Escherichia coli, Klebsiella sp, Shigella flexneri).For the investigation was used as extraction solvent a mixture of aIcohol-acetona (1:1), the biological activity of the obtained extracts was valued using the disc diffusion technique.The fruit peel of Caesalpinia spinosa and the leaves of Eucalyptus sp show a selective activity over the valued grampositives bacteriums.Se evalúo in vitro la actividad antibacteriana de extractos de Caesalpinia spinosa "tara" y Eucalyptus sp. "eucalipto" utilizando cepas bacterianas Gram positivas (Staphylococcus aureus y Bacillus subtilis) y Gram negativas (Escherichia coli, Klebsiella sp. y Shigella flexneri). Se utilizó como solvente de extracción una mezcla de alcohol-acetona (1:1) y la actividad biológica de los extractos obtenidos se evaluó mediante la técnica de difusión en disco.La cáscara del fruto de Caesalpinia spinosa y las hojas del Eucalyptus sp. mostraron una actividad selectiva sobre las bacterias Gram positivas evaluadas

Primer aislamiento de Escherichia coli O157:H7 enterohemorrágica en el Perú

En Febrero del año 2001 como parte del «Estudio transversal de los agentes etiológicos de diarrea aguda» en la Macroregión Sur del país, el Laboratorio Referencial de Tacna aisló una cepa procedente de una muestra de heces de un lactante de 11 meses de edad con un cuadro de diarrea disentérica, identificándola como Escherichia coli O157. Esta cepa fue confirmada y caracterizada en el Instituto Nacional de Salud como E. coli O157:H7 toxina shiga tipo II, siendo el primer aislamiento reportado de Escherichia coli enterohemorrágica en el Perú

Peruvian Vibrio cholerae O1 El Tor strains possess a distinct region in the Vibrio seventh pandemic island-II that differentiates them from the prototype seventh pandemic El Tor strains

A collection of environmental and clinical strains of Vibrio cholerae O1 isolated from the beginning of the Latin American epidemic of cholera in 1991 to 2003 from multiple locations in Peru were characterized and compared with V. cholerae O1 El Tor strains of the seventh pandemic from the rest of the world (Asia, Africa, Australia and Europe) using a multilocus virulence gene profiling strategy and DNA sequencing. Peruvian strains differed from El Tor strains from the rest of the world by the failure of PCR to amplify genes VC0512, VC0513, VC0514 and VC0515 in the Vibrio seventh pandemic island-II (VSP-II) gene cluster. Sequencing of the VSP-II gene cluster and its flanking regions in one Peruvian strain (PERU-130) confirmed the PCR results, indicating that the Peruvian strain had low DNA homology (46.6%) compared to the reference strain N16961 within the VSP-II region encompassing genes VC0511 to VC0515. Based on these differences in VSP-II, and based on the overall similarity between the pulsotypes of the Peruvian strains and the El Tor reference strain N16961, we concluded that the Peruvian, Eurasian and African strains belonged to the same clonal complex, and that the Peruvian strains represented variants that had independently evolved for a relatively short time. Since these ORFs in VSP-II of Peruvian strains are unique and conserved, they could form the basis for tracking the origin of the Peruvian strains and therefore of the Latin American pandemic