Correlation between Clinicopathology and Expression of Heat Shock Protein 72 and Glycoprotein 96 in Human Esophageal Squamous Cell Carcinoma

Heat shock protein 72 (HSP72) and glycoprotein 96 (gp96) are highly expressed in cancer tissues. Recent studies indicate the possible roles of HSP72 and gp96 in the development and progression of gastrointestinal carcinomas but detailed information is still ambiguous. We investigated the correlation between clinicopathology and expression of HSP72 and gp96 in human esophageal squamous cell carcinoma. The expression of HSP72 and gp96 was studied in 120 human esophageal squamous cell carcinomas with or without metastasis as well as in mucous membrane adjacent to cancers by way of immunohistochemistry. HSP72 immunoreactivities were detected in 112 of 120 primary tumors (93.3%) and in 30 of 120 mucous membranes adjacent to cancers (25.0%). Gp96 detected in esophageal squamous cell carcinoma and inmucous membrane adjacent to cancer was 85.0% and 20.0%, respectively. Both HSP72 and gp96 stained in cytoplasm. HSP72 and gp96 expression in esophageal squamous cell carcinomas withmetastasis was significantly higher than those with nonmetastasis (P < .05). The results indicate that there exists a significant correlation between the expression of HSP72 and gp96 and the progression of esophageal squamous cell carcinomas. HSP72 and gp96 expression were significantly associated with the presence of tumor infiltration, lymph node, and remote metastasis

Multiple roles of dihomo-γ-linolenic acid against proliferation diseases

Considerable arguments remain regarding the diverse biological activities of polyunsaturated fatty acids (PUFA). One of the most interesting but controversial dietary approaches focused on the diverse function of dihomo-dietary γ-linolenic acid (DGLA) in anti-inflammation and anti-proliferation diseases, especially for cancers. This strategy is based on the ability of DGLA to interfere in cellular lipid metabolism and eicosanoid (cyclooxygenase and lipoxygenase) biosynthesis. Subsequently, DGLA can be further converted by inflammatory cells to 15-(S)-hydroxy-8,11,13-eicosatrienoic acid and prostaglandin E1 (PGE1). This is noteworthy because these compounds possess both anti-inflammatory and anti-proliferative properties. PGE1 could also induce growth inhibition and differentiation of cancer cells. Although the mechanism of DGLA has not yet been elucidated, it is significant to anticipate the antitumor potential benefits from DGLA

MIClique: An Algorithm to Identify Differentially Coexpressed Disease Gene Subset from Microarray Data

Computational analysis of microarray data has provided an effective way to identify disease-related genes. Traditional disease gene selection methods from microarray data such as statistical test always focus on differentially expressed genes in different samples by individual gene prioritization. These traditional methods might miss differentially coexpressed (DCE) gene subsets because they ignore the interaction between genes. In this paper, MIClique algorithm is proposed to identify DEC gene subsets based on mutual information and clique analysis. Mutual information is used to measure the coexpression relationship between each pair of genes in two different kinds of samples. Clique analysis is a commonly used method in biological network, which generally represents biological module of similar function. By applying the MIClique algorithm to real gene expression data, some DEC gene subsets which correlated under one experimental condition but uncorrelated under another condition are detected from the graph of colon dataset and leukemia dataset

Mediating Role Of Socialization Towards Relationship Between Personality And Job Satisfaction

This research aims to explore the mediating effect of socialization on the relationship between personality and job satisfaction. Furthermore, to explore which personality traits can serve as predictors towards job satisfaction, targeting the bank branch managers in Pakistan by questionnaire in this Cross Section study. From the analysis, we found: (1) socialization has a mediating effect on the relationship between personality and job satisfaction, (2) Among five factors of Personality, Extraversion, Conscientiousness and Neuroticism are found to be significant predictors to determine Job Satisfaction. In conclusion, this research discusses the theoretical and practical connotations of the research results and proposed recommendations for follow-up studies

Immune checkpoint inhibitors-related pancreatitis with fulminant type 1 diabetes mellitus: case report and literature review

Immune checkpoint inhibitors (ICIs) are increasingly being used in the treatment of advanced human malignancies. ICIs-related adverse events, including pancreatitis and diabetes, have been individually characterized in the literature. The co-occurrence of ICIs-related pancreatitis with diabetes is rare and easily overlooked, but it is often severe or fatal. We present a patient with renal tumor resection who was treated with injection of the PD-L1 inhibitor toripalimab and eventually developed acute pancreatitis and fulminant type 1 diabetes mellitus. In addition, we conducted a literature review of ICIs-related pancreatitis with diabetes. The case in our report presented with paroxysmal abdominal pain and loss of appetite. Intravenous fluids and insulin infusion improved the patient’s pancreatitis and explosive hyperglycemia. This article suggests that ICIs can affect endocrine and exocrine functions of the pancreas, while providing information and new perspectives for the diagnosis and treatment of this challenging rare disease, helping inspire clinicians for the early identification and effective management of similar cases

Case Report: Identification of a novel LYN::LINC01900 transcript with promyelocytic phenotype and TP53 mutation in acute myeloid leukemia

Acute myeloid leukemia (AML) is a malignant disease of myeloid hematopoietic stem/progenitor cells characterized by the abnormal proliferation of primitive and naive random cells in the bone marrow and peripheral blood. Acute promyelocytic leukemia (APL) is a type (AML-M3) of AML. Most patients with APL have the characteristic chromosomal translocation t(15; 17)(q22; q12), forming PML::RARA fusion. The occurrence and progression of AML are often accompanied by the emergence of gene fusions such as PML::RARA, CBFβ::MYH11, and RUNX1::RUNX1T1, among others. Gene fusions are the main molecular biological abnormalities in acute leukemia, and all fusion genes act as crucial oncogenic factors in leukemia. Herein, we report the first case of LYN::LINC01900 fusion transcript in AML with a promyelocytic phenotype and TP53 mutation. Further studies should address whether new protein products may result from this fusion, as well as the biological function of these new products in disease occurrence and progression

Metagenomic insights into the composition and function of the gut microbiota of mice infected with Toxoplasma gondii

IntroductionDespite Toxoplasma gondii infection leading to dysbiosis and enteritis, the function of gut microbiota in toxoplasmosis has not been explored.MethodsHere, shotgun metagenomics was employed to characterize the composition and function of mouse microbial community during acute and chronic T. gondii infection, respectively.ResultsThe results revealed that the diversity of gut bacteria was decreased immediately after T. gondii infection, and was increased with the duration of infection. In addition, T. gondii infection led to gut microbiota dysbiosis both in acute and chronic infection periods. Therein, several signatures, including depression of Firmicutes to Bacteroidetes ratio and infection-enriched Proteobacteria, were observed in the chronic period, which may contribute to aggravated gut inflammation and disease severity. Functional analysis showed that a large amount of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and carbohydrate-active enzymes (CAZy) family displayed distinct variation in abundance between infected and healthy mice. The lipopolysaccharide biosynthesis related pathways were activated in the chronic infection period, which might lead to immune system imbalance and involve in intestinal inflammation. Moreover, microbial and functional spectrums were more disordered in chronic than acute infection periods, thus implying gut microbiota was more likely to participate in disease process in the chronically infected mice, even exacerbated immunologic derangement and disease progression.DiscussionOur data indicate that the gut microbiota plays a potentially important role in protecting mice from T. gondii infection, and contributes to better understand the association between gut microbiota and toxoplasmosis

22.運動負荷時の血小板機能とヘモレオロジー(第636回千葉医学会例会・第3回千葉大学第三内科懇話会)

Additional file 2. Changes of residual nitrate concentration during the experiments

Effect of Shear Deformation on Texture Evolution in Rolled Pure Titanium

The cold rolling texture evolution under different strain states caused by coupled effects from different combinations of roll gap geometry and friction in pure titanium with initial typical recrystallized texture has been studied using viscoplastic self-consistent simulations. Under plane strain state, the texture is dominated by a typical cold rolling fiber texture RD// in pure titanium. However, when shear deformation is induced and increased, RD// fiber texture is decreased, whereas basal texture (0001)[1010] is increased and a strong partial fiber texture RD// is formed. The variation of cold rolling texture at different strain states can be ascribed to variation of relative contribution from activation of prismatic, basal and pyramidal slip

Microstructure, Hardness Evolution, and Thermal Stability Mechanism of Mechanical Alloyed Cu-Nb Alloy during Heat Treatment

The microstructure, hardness evolution, and thermal stability of mechanically alloyed (MA-ed) nanocrystalline Cu–10 wt %Nb solid solution during heat treatment were examined using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution TEM (HRTEM) observations, and microhardness measurement. It is found that the pronounced precipitation of Nb from the Cu-Nb supersaturated solid solution occurs at temperatures up to 700 °C, and the annealed alloy shows a bi-nanostructure with Nb nanoparticles dispersed in the nanocrystalline Cu matrix. The bi-nanostructure remains stable with Cu crystalline grain size below 100 nm and Nb particle size around 10 nm even after annealing at 900 °C for 3 h. The microhardness of the annealed sample shows a small increase after annealing at 400 °C, and then it shows a slow decreasing trend with further increasing temperatures. With the help of the kinetics analyses, it is found that the coarsening of the stable Nb nanoparticles is controlled by volume diffusion. The enhanced stability of the nanocrystalline Cu microstructure is mainly attributed to the solute drag and precipitate pinning effects