HSP70s: From Tumor Transformation to Cancer Therapy

Heat shock proteins (HSPs) are a defined set of chaperones for maintaining proper functions of proteins. The HSP70 family, one of the most inducible families in response to stress, protects cells from stress-induced cell death. It has been documented that HSP70s are highly expressed in various types of cancer cells and make the cells resistant to adverse microenvironments, such as hypoxia and glucose starvation, which are common features in malignant progression. Over-expression of HSP70s is thus associated with tumor transformation and eventually results in a decrease of chemotherapy efficacy. Notably, the distribution of HSP70s is deregulated in cancer cells. It has been reported that HSP70s localize distinct organelles or are exported to humoral circulation during cancer development. Either surface or exported HSP70s play danger signals and trigger immune response to destroy the tumor cells. In this review, we lay out recent advances in the HSP70s-mediated cancer diagnosis and therapy. This review would be enlightening for clinical cancer medicine

Modeling Chinese post-90\u272 tourism loyalty to the ex-rival state using the perceived value approach

Purpose – A recent trend in tourism research involves the study of independent Chinese tourists. Yet while post-90s or Generation Z (i.e., born in the 1990s) comprises an important share of nondomestic visitors to Taiwan and other tourist destinations, this segment of the tourist population is currently under-analyzed. As a pioneering piece of research in this area, this survey attempts to understand Chinese tourists of this cohort visiting the long-divided state. Design – This research incorporates the social dimension of perceived value in the ordinarily employed perceived-value model to better understand why Chinese post-90s would like to recommend Taiwan. Methodology – This study samples Chinese students from 12 universities located in the northern, central, southern, and western regions of Taiwan. They were investigated with the selfadministered survey which is composed of five constructs, for a total of 17 questions. Structural equation modeling was employed to analyze the collected data and testify the hypotheses. Findings – The finding provides insights in the specific tourism behaviors of this cohort and how they are found distinct from their predecessors. The emotional dimension of the post-90s’ tourismrelated perceived value is a strong determinant of their loyalty to Taiwan as a tourist destination. The prior-rival situation between both sides of the Taiwan Strait might signify that social dimension significantly predict their loyalty, through the mediator of satisfaction. Originality – This research provides important information for tourism businesses regarding place management and marketing strategies, enabling them to receive this new generation of Chinese customers

Application of AHP hierarchical analysis and KANO quality model to investigate the needs of eye drop users and the charm factor of the compliance aids design

Previous studies have indicated that many patients with eye diseases are not adherent to eyedrops due to the difficulty in administration. The purpose of using eyedrop compliance aids is to help patients who have difficulties in administering eyedrops. However, this method has not been well recognized and accepted. This study aimed to upgrade eyedrop compliance aids so that the adherence of eyedrop users could be improved. Among the research methods employed in this study, the analytic hierarchy process (AHP) helped in proposing the best function sequence of the device, and a questionnaire based on the Kano model helped understand the degree of satisfaction with the existing products, as well as discover the appealing factors of their functions and examine their functional relationships. Finally, the results of the two methods were discussed and compared, and the results were used to identify the basic appealing factors influencing consumer satisfaction as well as provide the element attributes and significant features to be weighed in the design of eyedrops. This study suggested that in the design of eyedrops, both the physical and psychological needs of users should be taken into consideration, and reference indexes for aids design that may effectively improve user adherence to eyedrops should be proposed

IsaB Inhibits Autophagic Flux to Promote Host Transmission of Methicillin-Resistant Staphylococcus aureus.

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a major nosocomial pathogen that is widespread in both health-care facilities and in the community at large, as a result of direct host-to-host transmission. Several virulence factors are associated with pathogen transmission to naive hosts. Immunodominant surface antigen B (IsaB) is a virulence factor that helps Staphylococcus aureus to evade the host defense system. However, the mechanism of IsaB on host transmissibility remains unclear. We found that IsaB expression was elevated in transmissible MRSA. Wild-type isaB strains inhibited autophagic flux to promote bacterial survival and elicit inflammation in THP-1 cells and mouse skin. MRSA isolates with increased IsaB expression showed decreased autophagic flux, and the MRSA isolate with the lowest IsaB expression showed increased autophagic flux. In addition, recombinant IsaB rescued the virulence of the isaB deletion strain and increased the group A streptococcus (GAS) virulence in vivo. Together, these results reveal that IsaB diminishes autophagic flux, thereby allowing MRSA to evade host degradation. These findings suggest that IsaB is a suitable target for preventing or treating MRSA infection

Replication Attack Detection in Mobile Wireless Sensor Network with LEACH-ME Routing Protocol

Because the Wireless Sensor Network (WSN) nodes are low-cost devices, attackers may capture some nodes in this network and then duplicate these nodes to eavesdrop the transmitted messages or even control the network gradually without difficulty. This is the so-called node replication attack. This type of attacks could cause the huge threat to information security of WSNs. Therefore, in this paper, we would like to suggest a detection approach which can offer good performance but with higher energy consumption. Hence, it can provide an alternative solution for some specific applications that need better precision but do not care energy or lifespan too much

Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing

Introduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffolds to prolong the effective drug delivery of vildagliptin and insulin for the repair of diabetic wounds were prepared.Methods: To fabricate core-shell nanofibrous membranes, vildagliptin mixture with PLGA, and insulin solution were pumped via separate pumps into two differently sized capillary tubes that were coaxially electrospun.Results and Discussion: Nanofibrous core-shell scaffolds slowly released effective vildagliptin and insulin over 2 weeks in vitro migration assay and in vivo wound-healing models. Water contact angle (68.3 ± 8.5° vs. 121.4 ± 2.0°, p = 0.006) and peaked water absorbent capacity (376% ± 9% vs. 283% ± 24%, p = 0.003) of the insulin/vildagliptin core-shell nanofibrous membranes remarkably exceeded those of a control group. The insulin/vildagliptin-loaded core-shell nanofibers improved endothelial progenitor cells migration in vitro (762 ± 77 cells/mm2 vs. 424.4 ± 23 cells/mm2, p < 0.001), reduced the α-smooth muscle actin content in vivo (0.72 ± 0.23 vs. 2.07 ± 0.37, p < 0.001), and increased diabetic would recovery (1.9 ± 0.3 mm2 vs. 8.0 ± 1.4 mm2, p = 0.002). Core-shell insulin/vildagliptin-loaded nanofibers extend the drug delivery of insulin and vildagliptin and accelerate the repair of wounds associated with diabetes

Noninvasive prediction of Blood Lactate through a machine learning-based approach.

We hypothesized that blood lactate concentration([Lac]blood) is a function of cardiopulmonary variables, exercise intensity and some anthropometric elements during aerobic exercise. This investigation aimed to establish a mathematical model to estimate [Lac]blood noninvasively during constant work rate (CWR) exercise of various intensities. 31 healthy participants were recruited and each underwent 4 cardiopulmonary exercise tests: one incremental and three CWR tests (low: 35% of peak work rate for 15 min, moderate: 60% 10 min and high: 90% 4 min). At the end of each CWR test, venous blood was sampled to determine [Lac]blood. 31 trios of CWR tests were employed to construct the mathematical model, which utilized exponential regression combined with Taylor expansion. Good fitting was achieved when the conditions of low and moderate intensity were put in one model; high-intensity in another. Standard deviation of fitting error in the former condition is 0.52; in the latter is 1.82 mmol/liter. Weighting analysis demonstrated that, besides heart rate, respiratory variables are required in the estimation of [Lac]blood in the model of low/moderate intensity. In conclusion, by measuring noninvasive cardio-respiratory parameters, [Lac]blood during CWR exercise can be determined with good accuracy. This should have application in endurance training and future exercise industry