Media 1: In vivo fast variable focus photoacoustic microscopy using an electrically tunable lens

Originally published in Optics Express on 25 August 2014 (oe-22-17-20130

Media 2: In vivo fast variable focus photoacoustic microscopy using an electrically tunable lens

Originally published in Optics Express on 25 August 2014 (oe-22-17-20130

<i>In Vivo</i> Imaging-Guided Photothermal/Photoacoustic Synergistic Therapy with Bioorthogonal Metabolic Glycoengineering-Activated Tumor Targeting Nanoparticles

Developing multifunctional phototheranostics with nanoplatforms offers promising potential for effective eradication of malignant solid tumors. In this study, we develop a multifunctional phototheranostic by combining photothermal therapy (PTT) and photoacoustic therapy (PAT) based on a tumor-targeting nanoagent (DBCO-ZnPc-LP). The nanoagent DBCO-ZnPc-LP was facilely prepared by self-assembling of a single lipophilic near-infrared (NIR) dye zinc­(II)-phthalocyanine (ZnPc) with a lipid-poly­(ethylene glycol) (LP) and following modified further with dibenzyl cyclootyne (DBCO) for introducing the two-step chemical tumor-targeting strategy based on metabolic glycoengineering and click chemistry. The as-prepared DBCO-ZnPc-LP could not only convert NIR light into heat for effective thermal ablation but also induce a thermal-enhanced ultrasound shockwave boost to trigger substantially localized mechanical damage, achieving synergistic antitumor effect both <i>in vitro</i> and <i>in vivo</i>. Moreover, DBCO-ZnPc-LP can be efficiently delivered into tumor cells and solid tumors after being injected intravenously <i>via</i> the two-step tumor-targeting strategy. By integrating the targeting strategy, photoacoustic imaging, and the synergistic interaction between PTT and PAT, a solid tumor could be accurately positioned and thoroughly eradicated <i>in vivo</i>. Therefore, this multifunctional phototheranostic is believed to play an important role in future oncotherapy by the enhanced synergistic effect of PTT and PAT under the guidance of photoacoustic imaging

An H₂S‑Regulated Artificial Nanochannel Fabricated by a Supramolecular Coordination Strategy

Hydrogen sulfide (H2S), as the third gasotransmitter, has an important impact on physiological and pathological activities. Herein, we fabricated an artificial nanochannel with a conductance value of 2.01 nS via a supramolecular coordination strategy. Benefiting from the unique H2S-mediated covalent reaction, the nanochannel biosensor could change from ON to OFF states with the addition of H2S. Furthermore, this nanochannel directed the ion transport, showing a high rectification ratio as well as gating ratio. Subsequently, theoretical simulations were conducted to help to reveal the possible mechanism of the functionalized nanochannel. This study can provide insights for better understanding the process of H2S-regulated biological channels and fabricating gas gated nanofluids

An H₂S‑Regulated Artificial Nanochannel Fabricated by a Supramolecular Coordination Strategy

Hydrogen sulfide (H2S), as the third gasotransmitter, has an important impact on physiological and pathological activities. Herein, we fabricated an artificial nanochannel with a conductance value of 2.01 nS via a supramolecular coordination strategy. Benefiting from the unique H2S-mediated covalent reaction, the nanochannel biosensor could change from ON to OFF states with the addition of H2S. Furthermore, this nanochannel directed the ion transport, showing a high rectification ratio as well as gating ratio. Subsequently, theoretical simulations were conducted to help to reveal the possible mechanism of the functionalized nanochannel. This study can provide insights for better understanding the process of H2S-regulated biological channels and fabricating gas gated nanofluids

MOESM8 of Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii

Additional file 8: Table S7. Primer pairs used in qRT-PCR analysis

MOESM4 of Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii

Additional file 4: Table S3. List of differentially regulated proteins in butanol treatment

MOESM5 of Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii

Additional file 5: Table S4. GO annotation of proteins detected in this study

MOESM6 of Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii

Additional file 6: Table S5. GO enrichment analysis of differentially regulated proteins in ethanol treatment

MOESM2 of Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii

Additional file 2: Table S1. Accession numbers of proteins detected in this study