1 research outputs found
Antioxidant Dipeptides Enhance Osmotic Stress Tolerance by Regulating the Yeast Cell Wall and Membrane
This
study aimed to investigate the role of the yeast cell wall
and membrane in enhancing osmotic tolerance by antioxidant dipeptides
(ADs) including Ala-His (AH), Thr-Tyr (TY), and Phe-Cys (FC). Results
revealed that ADs could improve the integrity of the cell wall by
restructuring polysaccharide structures. Specifically, FC significantly
(p < 0.05) reduced the leakage of nucleic acid
and protein by 2.86% and 5.36%, respectively, compared to the control.
In addition, membrane lipid composition played a crucial role in enhancing
yeast tolerance by ADs, including the increase of cell membrane integrity
and the decrease of permeability by regulating the ratio of unsaturated
fatty acids. The up-regulation of gene expression associated with
the cell wall integrity pathway (RLM1, SLT2, MNN9, FKS1, and CHS3) and fatty acid biosynthesis (ACC1, HFA1, OLE1, ERG1, and FAA1) further confirmed the positive impact of ADs on yeast tolerance
against osmotic stress