46 research outputs found
Environmental Adaptation: Genomic Analysis of the Piezotolerant and Psychrotolerant Deep-Sea Iron Reducing Bacterium Shewanella piezotolerans WP3
Shewanella species are widespread in various environments. Here, the genome sequence of Shewanella piezotolerans WP3, a piezotolerant and psychrotolerant iron reducing bacterium from deep-sea sediment was determined with related functional analysis to study its environmental adaptation mechanisms. The genome of WP3 consists of 5,396,476 base pairs (bp) with 4,944 open reading frames (ORFs). It possesses numerous genes or gene clusters which help it to cope with extreme living conditions such as genes for two sets of flagellum systems, structural RNA modification, eicosapentaenoic acid (EPA) biosynthesis and osmolyte transport and synthesis. And WP3 contains 55 open reading frames encoding putative c-type cytochromes which are substantial to its wide environmental adaptation ability. The mtr-omc gene cluster involved in the insoluble metal reduction in the Shewanella genus was identified and compared. The two sets of flagellum systems were found to be differentially regulated under low temperature and high pressure; the lateral flagellum system was found essential for its motility and living at low temperature
PgtE Enzyme of Salmonella enterica Shares the Similar Biological Roles to Plasminogen Activator (Pla) in Interacting With DEC-205 (CD205), and Enhancing Host Dissemination and Infectivity by Yersinia pestis
Yersinia pestis, the cause of plague, is a newly evolved Gram-negative bacterium. Through the acquisition of the plasminogen activator (Pla), Y. pestis gained the means to rapidly disseminate throughout its mammalian hosts. It was suggested that Y. pestis utilizes Pla to interact with the DEC-205 (CD205) receptor on antigen-presenting cells (APCs) to initiate host dissemination and infection. However, the evolutionary origin of Pla has not been fully elucidated. The PgtE enzyme of Salmonella enterica, involved in host dissemination, shows sequence similarity with the Y. pestis Pla. In this study, we demonstrated that both Escherichia coli K-12 and Y. pestis bacteria expressing the PgtE-protein were able to interact with primary alveolar macrophages and DEC-205-transfected CHO cells. The interaction between PgtE-expressing bacteria and DEC-205-expressing transfectants could be inhibited by the application of an anti-DEC-205 antibody. Moreover, PgtE-expressing Y. pestis partially re-gained the ability to promote host dissemination and infection. In conclusion, the DEC-205-PgtE interaction plays a role in promoting the dissemination and infection of Y. pestis, suggesting that Pla and the PgtE of S. enterica might share a common evolutionary origin.Peer reviewe
Kinematic evolution of fold-and-thrust belts in the Yubei-Tangbei area: Implications for tectonic events in the southern Tarim Basin
The Yubei-Tangbei area in the southern Tarim Basin is one of the best-preserved Early Paleozoic northeast-southwest trending fold-and-thrust belts within this basin. This area is crucial for the exploration of primary hydrocarbon reservoirs in northwestern China. In this study, we constructed the structural geometric morphology of the Yubei-Tangbei area using geophysical logs, drilling, and recent two- and three-dimensional (2-D and 3-D) seismic data. The Early Paleozoic fault-propagation folds, the Tangnan triangle zone, fault-detachment folds, and trishear fault-propagation folds developed with the detachment of the Middle Cambrian gypsum–salt layer. According to a detailed chronostratigraphic framework, the growth strata in the Upper Ordovician–Lower Silurian layer formed by onlapping the back limb of the asymmetric fault-propagation folds, which therefore defines the timing of deformations. The changes in kink band hinges and amplitudes in the Permian–Carboniferous and Cenozoic folding strata suggest that the evolution of the fold-and-thrust belts followed a sequential evolution process rather than a simultaneous one. Above the pre-existing Precambrian basement structure, the Yubei-Tangbei fold-and-thrust belts can be divided into four tectonic evolution stages: Late Cambrian, Late Ordovician to Early Carboniferous, Carboniferous to Permian, and Cenozoic. The northwestern-verging Cherchen Fault is part of the piedmont fold-and-thrust system of the southern Tarim foreland basin. We interpreted its strata as a breakthrough trishear fault-propagation fold that developed in three phases: Mid–Late Ordovician, Silurian to Middle Devonian, and Triassic to present. These tectonic events are responses of the Altyn-Tagh and Kunlun collisional orogenic belts and the Indian-Eurasian collision. The inherited deformation and structural modification in the southern Tarim Basin may be an indicator of the growth and evolution of peripheral orogens
Synthesis, Characterization, and Anti-Phytopathogen Evaluation of 6-Oxychitosan Derivatives Containing N-Quaternized Moieties in Its Backbone
The structure modification of chitosan has great application potential. 6-Oxychitosan was prepared by specially oxidizing the C6-OH of chitosan, then 6-oxychitosan was reacted with three kinds of aldehydes to prepare N-quaternized 6-oxychitosan derivatives in this paper. The derivatives were characterized by FT-IR, NMR, and elemental analysis. The antimicrobial activity of these derivatives was tested against two common plant-threatening fungi and three plant disease bacteria. The results showed that N-quaternized 6-oxychitosan derivatives had good water-solubility and excellent antimicrobial activity. Moreover, derivative 3 which connected 8-hydroxyquinolines had the highest antimicrobial activity than the other derivatives. The inhibitory indices of derivative 3 against V. albo-atrum and P. hibernalis are 89.1% and 72.8% at 0.4 mg/ml. The MICs of 3 against X. oryzae, P. syringae, and E. rhapontici were 625, 625, and 156 mg/l, respectively. All the results indicate that derivative 3 has the potential of becoming an alternative to harmful agricultural chemicals
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Synthesis, Characterization, and Anti-Phytopathogen Evaluation of 6-Oxychitosan Derivatives Containing N-Quaternized Moieties in Its Backbone
Characterization of Different Salt Forms of Chitooligosaccharides and Their Effects on Nitric Oxide Secretion by Macrophages
In this paper, chitooligosaccharides in different salt forms, such as chitooligosaccharide lactate, citrate, adipate, etc., were prepared by the microwave method. They were characterized by SEM, FTIR, NMR, etc., and the nitric oxide (NO) expression was determined in RAW 264.7 cells. The results showed that pure chitooligosaccharide was an irregular spherical shape with rough surface, and its different salt type products are amorphous solid with different honeycomb sizes. In addition to the characteristic absorption peaks of chitooligosaccharides, in FTIR, the characteristic absorption of carboxyl group, methylene group, and aromatic group in corresponding acid appeared. The characteristic absorption peaks of carbon in carboxyl group, hydrogen and carbon in methyl, methylene group, and aromatic group in corresponding acid also appeared in NMR. Therefore, the sugar ring structure and linking mode of chitooligosaccharides did not change after salt formation of chitooligosaccharides. Different salt chitooligosaccharides are completely different in promoting NO secretion by macrophages, and pure chitooligosaccharides are the best
Laser Coherent Combination With Circular Array of Airy Beams
A high-efficiency laser coherent combination method based on the virtue of the self-transverse acceleration effect of Airy beams is proposed and demonstrated by converting an array of Gaussian beams into Airy beams array to reduce laser beams separation distance. The coherent combination procedures of circular array for both Airy and Gaussian beams are theoretically and experimentally studied. The results show that the central intensity of the laser coherent combination of the circular array of Airy beams is 14.33 times higher than that of the Gaussian beams array, and the combined efficiency of the circular array of Airy beams is 1.64 times higher than that of the Gaussian beams circular array. The method may provide an efficient way to increase combination efficiency of laser coherent combination array and may have a broad application prospect in optical communication, particle capture, material processing
Efficient Therapeutic Function and Mechanisms of Human Polyclonal CD8+CD103+Foxp3+ Regulatory T Cells on Collagen-Induced Arthritis in Mice
Objective. To investigate the potential therapeutic effect in a rheumatoid arthritis model of stable human CD8+ regulatory T cells (hCD8+Tregs) induced by TGF-β1 and rapamycin (RAPA) in vitro. Methods. Human CD8+T cells were isolated from human peripheral blood mononuclear cells and induced/expanded with TGF-β1 and RAPA along with anti-CD3/28 beads and IL-2 in vitro and harvested as hCD8+Tregs. The phenotypes, suppressive characteristics, and stability of the hCD8+Tregs in an inflammatory microenvironment were examined in vitro. Human CD8+Tregs were transfused into an acollagen-induced arthritis (CIA) mouse model, and their therapeutic effects and related mechanisms were investigated. Results. Human CD8+Tregs induced by TGF-β1/RAPA showed high expression of Foxp3 and CD103, exhibited vigorous suppression ability, and were stable in inflammatory microenvironments. In CIA mice, the clinical scores, levels of anti-collagen IgG antibody, and cartilage destruction were significantly reduced after adoptive transfusion with hCD8+Tregs. Moreover, hCD8+Treg treatment significantly reduced the number of Th17 cells, increased the number of CD4+IFN-γ+T cells, and produced self CD4+Foxp3+Tregs in vivo. In an in vitro cell coculture assay, hCD8+Tregs significantly inhibited mouse CD4+ effector T cell proliferation, induced mouse CD4+Foxp3+Treg and CD4+IFN-γ+Th1 cell production, reduced Th17 cell development, and downregulated CD80/86 expression on mature DCs (mDCs). Conclusion. TGF-β1/RAPA can induce hCD8+Tregs with stable suppressive characteristics, which could significantly alleviate the severity of CIA based on their stable suppressive ability in an inflammatory microenvironment and further influence the function of other downstream cell subtypes. Human CD8+Tregs might be a therapeutic strategy for rheumatoid arthritis