General genome features of a pheasant-origin avian orthoreovirus (ARV), the novel field variant of pheasant ARV (Reo/SDWF/Pheasant/17608/20).

General genome features of a pheasant-origin avian orthoreovirus (ARV), the novel field variant of pheasant ARV (Reo/SDWF/Pheasant/17608/20).</p

The illustrations of the homology search results for NGS reads and the sequencing coverage analysis.

A: Total NGS reads homology search result; B: The remapping of NGS raw reads to ARV 10 segments; C: The result of sequencing coverage analysis.</p

Sequence identities between the pheasant ARV (Reo/SDWF/Pheasant/17608/20) and reference strains of <i>Orthoreovirus</i> genus members.

Sequence identities between the pheasant ARV (Reo/SDWF/Pheasant/17608/20) and reference strains of Orthoreovirus genus members.</p

Genbank accession numbers of full genome segments of avian orthoreovirus (ARV) reference strains: PA05682, S1133, 138, 1733, 176, J18, BRoV, BRV, NBV, PuV, MRV1, MRV2 and MRV3.

(DOCX)</p

Comparison of segment 5’ and 3’ non-coding regions of the pheasant ARV (Reo/SDWF/Pheasant/17608/20) with reference strains of <i>Orthoreovirus</i> genus members.

Comparison of segment 5’ and 3’ non-coding regions of the pheasant ARV (Reo/SDWF/Pheasant/17608/20) with reference strains of Orthoreovirus genus members.</p

Phylogenetic trees constructed by avian orthoreovirus (ARV) based on nucleotide sequences of the L-class, M-class and σ-class homologous genome segments or genes.

Note: The Reo/SDWF /Pheasant/17608/20 strain was marked with a red color dot.</p

Amino acid alignment of p10 and p17 protein.

A: The p10 protein of Reo/SDWF /Pheasant/17608/20 align with the homologous protein of Reo/PA/Broiler/05682/12 (or PA05682), S1133, 1733, 138 and Reo/PA/Turkey/22342/13 (or PA22342) strains; B: The p17 protein of Reo/SDWF /Pheasant/17608/20 align with the homologous protein of Reo/PA/Broiler/05682/12 (or PA05682), S1133, 1733, 138 and Reo/PA/Turkey/22342/13 (or PA22342) strains.</p

The mVISTA method for whole genome nucleotide alignment.

Alignment result of the Reo/SDWF /Pheasant/17608/20 in comparisons with the Reo/PA/Broiler/05682/12 (or PA05682), S1133, 1733, 138, Reo/PA/Turkey/22342/13 (or PA22342) and J18 strains was illustrated; Areas in pink color represent ≥ 90% similarities; and areas in white represent < 90% similarities. The scale bar measures approximate length of the concatenated genome.</p