Influence of Elevation on Slake Durability Index of Quartz Mica Schist: A Case Study of East Slope of Manjiazhai Open Pit

In order to study the influence of elevation on the slake durability index of the quartz mica schist, the quartz mica schist with different elevations on the east slope of the Manjiazhai open-pit mine is taken as the research object, and based on laboratory tests and statistical analysis, the variation of negative correlation between the slake durability index and elevation is obtained. The disintegration mechanism of quartz mica schist at different elevations is also discussed. The test results show that the disintegration characteristics of quartz mica schist at different elevations are related to its mineral composition, fissure channel size, and rock damage effect. As the slope height increases, the ratio of mica to quartz in the rock increases, and the greater the porosity of the rock, the more fissures in the rock, the greater the permeability coefficient, and the more obvious the change of effective stress of rock under osmotic pressure. At the same time, the higher the slope elevation of open-pit mine, the longer the weathering time of rock, the higher the cumulative damage of rock, and the lower the rock slake durability index. This study provides a new idea for guiding the research on the disintegration characteristics of similar soft rock slopes in the elevation direction

Characterization of an Artificial Liver Support System-Related Vasovagal Reaction

Objective. An artificial liver support system (ALSS) is an effective therapy for patients with severe liver injury. A vasovagal reaction (VVR) is a common complication in various treatment settings but has not been reported previously in ALSS. Methods. This study retrospectively evaluated patients who suffered an ALSS-related VRR between January 2018 and June 2019. We collected data from VVR episodes including onset time, duration, changes in heart rate (HR) and blood pressure (BP), and drug treatment. Results. Among 637 patients who underwent ALSS treatment, 18 were included in the study. The incidence of VVR was approximately 2.82%. These patients were characterized by a rapid decrease in BP or HR with associated symptoms such as chest distress, nausea, and vomiting. The majority of patients (78%) suffered a VVR during their first ALSS treatment. Sixteen patients (89%) had associated symptoms after treatment began. Sixteen patients (89%) received human albumin or Ringer’s solution. Atropine was used in 11 patients (61%). The symptoms were relieved within 20 min in 15 patients and over 20 min in 3 patients. Conclusions. A VVR is a rare complication in patients with severe liver injury undergoing ALSS treatment. Low BP and HR are the main characteristics of a VVR

Novel Monomethoxy Poly(Ethylene Glycol) Modified Hydroxylated Tung Oil for Drug Delivery

Novel monomethoxy poly(ethylene glycol) (mPEG) modified hydroxylated tung oil (HTO), denoted as mPEG-HTO-mPEG, was designed and synthesized for drug delivery. mPEG-HTO-mPEG consists of a hydroxylated tung oil center joined by two mPEG blocks via a urethane linkage. The properties of mPEG-HTO-mPEG were affected by the length of the mPEG chain. Three mPEG with different molecular weights were used to prepare mPEG-HTO-mPEG. The obtained three mPEG-HTO-mPEG polymers were characterized by nuclear magnetic resonance (NMR), Fourier transformation infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and gel permeation chromatography (GPC), respectively. Furthermore, the particle sizes of mPEG-HTO-mPEG micelles were evaluated by dynamic light scattering (DLS) and transmission electron microscope (TEM). A critical aggregation concentration (CAC) ranged from 7.28 to 11.73 mg/L depending on the chain length of mPEG. The drug loading and release behaviors of mPEG-HTO-mPEG were investigated using prednisone acetate as a model drug, and results indicated that hydrophobic prednisone acetate could be effectively loaded into mPEG-HTO-mPEG micelles and exhibited a long-term sustained release. Moreover, compared with HTO, mPEG-HTO-mPEG had no obvious cytotoxicity to HeLa and L929 cells. Therefore, monomethoxy poly(ethylene glycol) modified hydroxylated tung oil mPEG-HTO-mPEG may be a promising drug carrier

Quantifying the distribution of ceria nanoparticles in cucumber roots:the influence of labeling

Study on the fluorescent-labeling-caused changes in the distribution and bio-effects of ceria nanoparticles.</p

Additional file 1 of Characteristics of circulating immune cells in HBV-related acute-on-chronic liver failure following artificial liver treatment

Additional file 1: Table S1. Cryopreservation and resuscitation of PBMCs. Table S2. CyTOF panel. Table S3. The classification of all CD45+ immune cells and the key marker expression. Fig. S1. Flow chart of inclusion of HBV-ACLF patients treated with ALSS. HBV-ACLF: hepatitis B virus-related acute-on-chronic liver failure; ALSS: artificial liver support system. Fig. S2. A. The proportion of naïve CD8+ T between improved and non-improved patients before and after ALSS therapy. B. The expression of PD1, CTLA4, CD69, CD27, CD127, and Granzyme B on effector CD8+ T cells in the two groups before and after treatment. C. The expression of PD1, CTLA4, CD69, CD27, CD127, and Granzyme B on naïve CD8+ T cells in the two groups before and after treatment. D. The frequency of C14, C15, and C16 (NK cells) clusters between improved and non-improved patients before and after ALSS therapy. NK: natural kill; ALSS: artificial liver support system. Fig. S3. A. Boxplots displaying the difference between immune cell subsets of two groups in total immune cell level before ALSS therapy. B. The proportion of classical, intermediate, and non-classical monocytes between improved and non-improved patients before and after ALSS therapy. C. The changes of granulocytes in peripheral blood of two groups before and after treatment. ALSS: artificial liver support system

Fate and Phytotoxicity of CeO₂ Nanoparticles on Lettuce Cultured in the Potting Soil Environment

<div><p>Cerium oxide nanoparticles (CeO₂ NPs) have been shown to have significant interactions in plants. Previous study reported the specific-species phytotoxicity of CeO₂ NPs by lettuce (<i>Lactuca sativa</i>), but their physiological impacts and vivo biotransformation are not yet well understood, especially in relative realistic environment. Butterhead lettuce were germinated and grown in potting soil for 30 days cultivation with treatments of 0, 50, 100, 1000 mg CeO₂ NPs per kg soil. Results showed that lettuce in 100 mg·kg^-1 treated groups grew significantly faster than others, but significantly increased nitrate content. The lower concentrations treatment had no impact on plant growth, compared with the control. However, the higher concentration treatment significantly deterred plant growth and biomass production. The stress response of lettuce plants, such as Superoxide dismutase (SOD), Peroxidase (POD), Malondialdehyde(MDA) activity was disrupted by 1000 mg·kg^-1 CeO₂ NPs treatment. In addition, the presence of Ce (III) in the roots of butterhead lettuce explained the reason of CeO₂ NPs phytotoxicity. These findings demonstrate CeO₂ NPs modification of nutritional quality, antioxidant defense system, the possible transfer into the food chain and biotransformation in vivo.</p></div

(A) Ce contents in roots (A) and leaves (B) of lettuce plants.

<p>Error bars stand for standard errors. Bar with the same letters show no statistically significant differences at p≤0.05. n = 8.</p

Longitudinal dynamic single-cell mass cytometry analysis of peripheral blood mononuclear cells in COVID-19 patients within 6 months after viral RNA clearance

Abstract This study investigates the longitudinal dynamic changes in immune cells in COVID-19 patients over an extended period after recovery, as well as the interplay between immune cells and antibodies. Leveraging single-cell mass spectrometry, we selected six COVID-19 patients and four healthy controls, dissecting the evolving landscape within six months post-viral RNA clearance, alongside the levels of anti-spike protein antibodies. The T cell immunophenotype ascertained via single-cell mass spectrometry underwent validation through flow cytometry in 37 samples. Our findings illuminate that CD8 + T cells, gamma-delta (gd) T cells, and NK cells witnessed an increase, in contrast to the reduction observed in monocytes, B cells, and double-negative T (DNT) cells over time. The proportion of monocytes remained significantly elevated in COVID-19 patients compared to controls even after six-month. Subpopulation-wise, an upsurge manifested within various T effector memory subsets, CD45RA + T effector memory, gdT, and NK cells, whereas declines marked the populations of DNT, naive and memory B cells, and classical as well as non-classical monocytes. Noteworthy associations surfaced between DNT, gdT, CD4 + T, NK cells, and the anti-S antibody titer. This study reveals the changes in peripheral blood mononuclear cells of COVID-19 patients within 6 months after viral RNA clearance and sheds light on the interactions between immune cells and antibodies. The findings from this research contribute to a better understanding of immune transformations during the recovery from COVID-19 and offer guidance for protective measures against reinfection in the context of viral variants

XANES Ce LIII-edge spectra (5723 eV) in roots of butterhead lettuce treated with CeO₂ NPs.

<p>The dotted line indicates the feature a, b and c.</p